1. School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China; 2. Institute of Environmental Health and Ecological Security, Jiangsu University, Zhenjiang 212013, China; 3. Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou 215009, China
Abstract：To enhance the anti-biofouling performance of polyacrylonitrile ultrafiltration membrane in wastewater, quorum sensing inhibitor, vanillin, was used to modify the membrane by surface coating. The surface morphology, chemistry, hydrophilicity, surface charge, and water permeability of the modified membrane were systematically characterized. Escherichia coli (E. coli) was used to assess the impact of vanillin on bacterial colony growth and biofilm formation on vanillin modified membrane. Effluent from the bioreactor was used as feed to test the anti-fouling performance of vanillin modified membrane. The results showed that vanillin could effectively inhibit the formation of bacterial colonies on agar plates. The initial adhesion and growth of E. coli on the modified membrane were decelerated, and there was no mature biofilm on the membrane surface after being cultured for 48h. Membrane fouling experiments showed that the flux reduction of the modified membrane was 15.6% less than that of the unmodified membrane. The extracellular polymeric substances content in the biofilm of the modified membrane was 15.9% less than that of the unmodified membrane, further demonstrating the enhanced anti-biofouling property after modification. The results indicated that the modified membrane reduced the accumulation of N-acyl homoserine lactones (AHLs) signal molecules in the filtration system, and inhibited the participation of dominant Enterobacteriaceae bacteria from feed in biofilm formation, which affected the structure of bacterial community in biofilm.
Zhao X, Zhang R, Liu Y, et al. Antifouling membrane surface construction: chemistry plays a critical role [J]. Journal of Membrane Science, 2018,551:145-171.
[2]
熊馨雅,曾 嘉,董梦婵,等.多巴胺非共价改性碳纳米管超滤膜的制备及抗污染性能 [J]. 中国环境科学, 2022,42(9):4207-4216. Xiong X Y, Zeng J, Dong M C, et al. Preparation and antifouling performance of dopamine non-covalently functionalized carbon nanotube ultrafiltration membranes [J]. Chinese Environmental Science, 2022,42(9):4207-4216.
[3]
Flemming H C, Wingender J. The biofilm matrix [J]. Nature Reviews Microbiology, 2010,8(9):623-633.
[4]
Maddela N R, Abiodun A S, Zhang S, et al. Biofouling in membrane bioreactors—mitigation and current status: a review [J]. Applied Biochemistry and Biotechnology, 2022,194:1-26.
[5]
Dickschat J S. Quorum sensing and bacterial biofilms [J]. Natural Product Reports, 2010,27(3):343-369.
[6]
周荣煊,马潇然,李 军,等.低温下AHLs对多级A/O工艺中好氧生物膜特性的影响 [J]. 中国环境科学, 2021,41(5):2133-2140. Zhou R X, Ma X R, Li J, et al. Effects of AHLs at low temperature on the characteristics of mature biofilms in aerobic zone of multi-stage A/O technology [J]. Chinese Environmental Science, 2021,41(5): 2133-2140.
[7]
Bassler B L. Small talk: cell-to-cell communication in bacteria [J]. Cell, 2002,109(4):421-424.
[8]
Lade H, Paul D, Kweon J H. N-acyl homoserine lactone-mediated quorum sensing with special reference to use of quorum quenching bacteria in membrane biofouling control [J]. BioMed Research International, 2014,2014:162584.
[9]
Kim S R, Oh H S, Jo S J, et al. Biofouling control with bead-entrapped quorum quenching bacteria in membrane bioreactors: physical and biological effects [J]. Environmental Science and Technology, 2013,47(2):836-842.
[10]
Yeon K M, Cheong W S, Oh H S, et al. Quorum sensing: a new biofouling control paradigm in a membrane bioreactor for advanced wastewater treatment [J]. Environmental Science and Technology, 2009,43(2):380-385.
[11]
Lee B, Yeon K M, Shim J, et al. Effective antifouling using quorum-quenching acylase stabilized in magnetically-separable mesoporous silica [J]. Biomacromolecules, 2014,15(4):1153-1159.
[12]
Kim T H, Lee I, Yeon K M, et al. Biocatalytic membrane with acylase stabilized on intact carbon nanotubes for effective antifouling via quorum quenching [J]. Journal of Membrane Science, 2018,554:357-365.
[13]
Nam A N, Kweon J H, Ryu J H, et al. Reduction of biofouling using vanillin as a quorum sensing inhibitory agent in membrane bioreactors for wastewater treatment [J]. Membrane Water Treatment, 2015,6(3): 189-203.
[14]
Kim J, Shin M, Song W, et al. Application of quorum sensing inhibitors for improving anti-biofouling of polyamide reverse osmosis membranes: Direct injection versus surface modification [J]. Separation and Purification Technology, 2021,255:117736.
[15]
程阳眷,黄 丹,王玉璠,等.环境介质对微生物群体感应信号分子的影响 [J]. 中国环境科学, 2023,43(4):2007-2016. Cheng Y J, Huang D, Wang Y F, et al. Influences of environmental substrates on microbial quorum-sensing signals [J]. China Environmental Science, 2023,43(4):2007-2016.
[16]
Ponnusamy K, Paul D, Kweon J H. Inhibition of quorum sensing mechanism and Aeromonas hydrophila biofilm formation by vanillin [J]. Environmental Engineering Science, 2009,26(8):1359-1363.
[17]
Kappachery S, Paul D, Yoon J, et al. Vanillin, a potential agent to prevent biofouling of reverse osmosis membrane [J]. Biofouling, 2010,26(6):667-672.
[18]
Ham S Y, Kim H S, Jang Y, et al. Control of membrane biofouling by 6-gingerol analogs: Quorum sensing inhibition [J]. Fuel, 2019,250: 79-87.
[19]
Katebian L, Hoffmann M R, Jiang S C. Incorporation of quorum sensing inhibitors onto reverse osmosis membranes for biofouling prevention in seawater desalination [J]. Environmental Engineering Science, 2018,35(4):261-269.
[20]
Wu Y C, Quan X C, Si X R. Incorporation of brominated furanone into Nafion polymer enhanced anti-biofilm efficacy [J]. International Biodeterioration & Biodegradation, 2015,99:39-44.
[21]
Hu Y, Lu K, Fang Y, et al. Enhancing the performance of aromatic polyamide reverse osmosis membrane by surface modification via covalent attachment of polyvinyl alcohol (PVA) [J]. Journal of Membrane Science, 2016,501:209-219.
[22]
Yan F, Chen H, Lü Y, et al. Improving the water permeability and antifouling property of thin-film composite polyamide nanofiltration membrane by modifying the active layer with triethanolamine [J]. Journal of Membrane Science, 2016,513:108-116.
[23]
王 蝶,王小洋,陈 曦,等.碳纳米管界面改性超滤膜的抗污机理解析 [J]. 中国环境科学, 2022,42(4):1618-1624. Wang D, Wang X Y, Chen X, et al. Anti-fouling mechanism for ultrafiltration membrane of modified surface by carbon nanotubes [J]. China Environmental Science, 2022,42(4):1618-1624.
[24]
Dubois M, Gilles K A, Hamilton J K, et al. Colorimetric method for determination of sugars and related substances [J]. Analytical Chemistry, 1956,28(3):350-356.
[25]
Zhang X, Bahtiar F, Wang Y, et al. Achieving simultaneous biological nutrient removal and sludge minimization from marine ship sewage based on an innovative Landscape Integrated Ecological Treatment System (LIETS) [J]. Ecological Engineering, 2020,156:105989.
[26]
Tong C Y, Derek C J C. Membrane surface roughness promotes rapid initial cell adhesion and long term microalgal biofilm stability [J]. Environmental Research, 2022,206:112602.
[27]
Tang B, Wu P. In situ study of diffusion and interaction of water and electrolyte solution in polyacrylonitrile (PAN) membrane using FTIR and two-dimensional correlation analysis [J]. Vibrational Spectroscopy, 2009,51(1):65-71.
[28]
Sathish Kumar R, Arthanareeswaran G, Lukka Thuyavan Y, et al. Enhancement of permeability and antibiofouling properties of polyethersulfone (PES) membrane through incorporation of quorum sensing inhibition (QSI) compound [J]. Journal of the Taiwan Institute of Chemical Engineers, 2017,72:200-212.
[29]
Lu Q, Li N. Preparation of hydrophilic polyvinylidene fluoride/ polyvinyl alcohol ultrafiltration membrane via polymer/non-solvent co-induced phase separation method towards enhance anti-fouling performance [J]. Journal of Environmental Chemical Engineering, 2021,9(6):106431.
[30]
Li R, Wu Y, Shen L, et al. A novel strategy to develop antifouling and antibacterial conductive Cu/polydopamine/polyvinylidene fluoride membranes for water treatment [J]. Journal of Colloid and Interface Science, 2018,531:493-501.
[31]
Huang J, Shi Y, Zeng G, et al. Acyl-homoserine lactone-based quorum sensing and quorum quenching hold promise to determine the performance of biological wastewater treatments: An overview [J]. Chemosphere, 2016,157:137-151.
[32]
李 点,董明盛,张秋勤.肠杆菌科细菌群体感应系统的研究进展 [J]. 生物加工过程, 2020,18(2):193-199. Li D, Dong M S, Zhang Q Q, et al. Research progress in quorum sensing system in Enterobacteriaceae [J]. Chinese Journal of Bioprocess Engineering, 2020,18(2):193-199.
[33]
Romero M, Avendaño H R, Magariños B, et al. Acylhomoserine lactone production and degradation by the fish pathogen Tenacibaculum maritimum, a member of the Cytophaga-Flavobacterium-Bacteroides (CFB) group [J]. FEMS Microbiology Letters, 2010,304(2):131-139.
