The electron transfer research of biogenic nano-palladium by Klebsiella strain
JU Cheng-jia1, WANG Zi-yang2, CAO Wen-ying1, YU Lei1,2, QIU Xu-hai3, ZHOU Shao-hua4, WANG Quan1, CHEN Rong-ping1,4
1. Department of Environmental Engineering, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China; 2. Soil Environment Research Institute, Jiangsu Provincial Academy of Environmental Science, Nanjing 210003, China; 3. Klebs Environmental Technology(Suzhou) Co., Ltd., Kunshan 215333, China; 4. Anhui Kejie Environmental Protection Technology Co., Ltd., Hefei 230088, China
Abstract：Chem-Pd (chemically synthesized palladium nanoparticle) and Bio-Pd (biosynthetic palladium nanoparticle) were prepared using chemical and biological methods, respectively, with sodium formate serving as the electron donor. The TEM and XRD results revealed that the Chem-Pd was agglomerated with an average particle size of 12nm, while Bio-Pd was evenly distributed inside the cell and in the extracellular polymeric substance (EPS), with an average size of 5nm. The reduction curve of bivalent palladium showed that the rate of bivalent palladium reduction by the biological method was 70% higher than that by the chemical method, indicating that the intervention of microorganisms can effectively promote the formation of nano palladium. By exploring the effect of intracellular electron transfer and extracellular polymer on the synthesis of Bio-Pd, the synthesis mechanism of Bio-Pd was clarified: the Klebsiella oxytoca straintransferred electrons to extracellular palladium divalent via its respiratory chain. Meanwhile, the presence of redox active groups in EPS may enhance Bio-Pd production outside the cell. Additionally, the test of live/dead bacteria showed that Bio-Pd remained harmful to cells.
居成佳, 汪子阳, 曹雯莹, 虞磊, 邱旭海, 周少华, 王权, 陈荣平. Klebisella还原合成纳米钯的电子传递途径解析[J]. 中国环境科学, 2023, 43(7): 3554-3561.
JU Cheng-jia, WANG Zi-yang, CAO Wen-ying, YU Lei, QIU Xu-hai, ZHOU Shao-hua, WANG Quan, CHEN Rong-ping. The electron transfer research of biogenic nano-palladium by Klebsiella strain. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(7): 3554-3561.
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