热水解污泥酸化液碳氮磷对PHA合成的影响

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摘要基于热水解污泥酸化液定向驯化得到了稳定的PHA合成混合菌体系，分析了菌群特征与结构，研究了限制氮、磷、非挥发性脂肪酸（non-VFAs）物质和补料方式对PHA合成率以及相关组分对菌群活性的影响.结果表明，利用热水解污泥酸化液合成PHA的特有混合菌体系中优势菌属短枝单胞菌属（Brachymonas）占45%.限制酸化液中NH+ 4-N浓度PHA合成率从22wt%提高至25wt%，限制non-VFAs不仅PHA合成率可提升27%同时合成速率也提升了25%.酸化液中的NH₄⁺-N、PO₄^3--P和non-VFAs未对PHA合成菌群活性造成显著影响，但是高浓度挥发性脂肪酸（VFAs）会对菌群活性造成抑制.为降低高浓度VFAs的抑制采取分批补料策略可提高PHA合成率，分批补料（5次）PHA最大合成率较一次补料的28wt%提升至34wt%.因此，通过提高酸化液中VFAs占比及优化补料方式均可提升PHA合成率，热水解污泥酸化液生物合成PHA在未来工业化生产时具有很大发展前景.

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	贺赟
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	姜大伟
	常菁
	郝姗
	王刚
	王佳伟

关键词 ：混合菌群, 热水解污泥, 聚羟基脂肪酸酯, 挥发性脂肪酸

Abstract：Based on the domestication of the acid liquor of fermented thermal-hydrolyzed sludge, the mixed microbial cultures for producing PHA were obtained and its characteristics were explored. The influence mechanism of feeding method and composition (i.e., N, P, non-VFAs) of the acid liquor on the PHA yield and microbial activity were also investigated. Specifically, the predominant Brachymonas accounted for 45% of the unique mixed microbial cultures that yield PHA. The PHA productivity was improved from 22wt% to 25wt% by limiting the concentration of NH+ 4-N in the acid liquor. By limiting the concentration of non-VFAs, not only the PHA productivity was improved 27% but also the production efficiency was improved 25%. Microbial activity was not effected significantly by non-VFAs、NH₄⁺-N and PO₄^3--P in acid liquor while was obviously restrained by volatile fatty acids (VFAs) with high concentration. To mitigate inhibition of VFAs and improve the PHA yield, intermittent feeding (5times) strategy was used. The PHA yield was effectively improved to 34wt% compared with 28wt% in disposable feeding. Furthermore, the PHA yield was improved by increasing the VFAs proportion in the acid liquor and optimizing the feeding method. The production of PHA by microorganism from fermented thermal-hydrolyzed sludge will be great promise in future industrialization.

Key words： mixed microbial cultures thermal-hydrolyzed sludge polyhydroxyalkanoates (PHA) volatile fatty acids (VFAs)

收稿日期: 2023-02-24

PACS:

X705

通讯作者: 李魁晓,教授级高级工程师,kuixiao_li@163.com E-mail: kuixiao_li@163.com

作者简介: 贺赟(1992-),女,陕西西安人,工程师,硕士,主要从事污水治理与资源化研究.发表论文5篇.heyun1116@163.com.

引用本文:

贺赟, 李魁晓, 姜大伟, 常菁, 郝姗, 王刚, 王佳伟. 热水解污泥酸化液碳氮磷对PHA合成的影响[J]. 中国环境科学, 2023, 43(10): 5181-5187. HE Yun, LI Kui-xiao, JIANG Da-wei, CHANG Jing, HAO Shan, WANG Gang, WANG Jia-wei. Effects of carbon, nitrogen and phosphorus in fermented thermal-hydrolyzed sludge on polyhydroxyalkanoates production. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(10): 5181-5187.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I10/5181

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