Effects of pH on the production of volatile fatty acids from citrus waste and waste activated sludge by anaerobic co-fermentation
SUN Hong1, XIAO Xiang-zhe1, LUO Jin-cai1, ZHANG Liang1, LIN Peng1, DONG Shan-yan1,2, ZHU Yi-chun1,2
1. School of Civil and Surveying &Mapping Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, China; 2. Research Center for Water Quality Security Technology at Ganjiang River Basin, Ganzhou 341000, China
Abstract：In order to improve the production of volatile fatty acids (VFA), the effects of different ratios of volatile solids(VS) from citrus waste and waste activated sludge and regulating pH in fermentation processes were studied. The results showed that adjusting the pH of the co-fermentation system to 6could promote the accumulation of VFA. When the ratio of VS from citrus waste and waste activated sludge reached 2, the maximum VFA yield was 11183.12mg/L, which was 1.75times higher than that without pH control. Furthermore, the essential oil from citrus waste could inhibit the methanogenic process, which was strengthened by controlling pH in the fermentation process. After adding citrus waste, the microbial community structure of the co-fermentation system showed significant advantages. VFA related microbial population increased significantly while methanogenic bacteria consuming acetic acid decreased, which was conducive to the accumulation of VFA. The results of this experiment can provide a theoretical basis for optimizing the performance of VFA production by anaerobic co-fermentation of citrus waste and waste activated sludge.
孙鸿, 肖向哲, 罗进财, 章亮, 林朋, 董姗燕, 朱易春. pH值调控对柑橘废渣与污泥厌氧共发酵产酸影响[J]. 中国环境科学, 2022, 42(4): 1762-1769.
SUN Hong, XIAO Xiang-zhe, LUO Jin-cai, ZHANG Liang, LIN Peng, DONG Shan-yan, ZHU Yi-chun. Effects of pH on the production of volatile fatty acids from citrus waste and waste activated sludge by anaerobic co-fermentation. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(4): 1762-1769.
Wei L, Zhu F, Li Q, et al. Development, current state and future trends of sludge management in China:Based on exploratory data and CO2-equivaient emissions analysis[J]. Environment international, 2020,144:106093.
[2]
王晓利,曾正中,王厚成,等.污泥处理处置及资源化方法探讨[J]. 环境工程, 2014,32(3):150-154. Wang X L, Zeng Z Z, Wang H C, et al. The disposal technique and comprehensive utilization of sludge[J]. Environmental Engineering, 2014,32(3):150-154.
[3]
Rao Y, Wan J, Liu Y, et al. A novel process for volatile fatty acids production from syngas by integrating with mesophilic alkaline fermentation of waste activated sludge[J]. Water Research, 2018,139:372-380.
[4]
李淑兰,刘萍,宋立.柑橘皮的基本特性及沼气化处理可行性研究[J]. 中国沼气, 2018,36(3):64-66. Li S L, Liu P, Song L. The basic characteristics of orange peel and its deasibility for biogas production treatment[J]. China Biogas, 2018, 36(3):64-66.
[5]
赵婷,姜鑫,陈永栋,等.剩余污泥与柑橘渣协同厌氧消化特性研究[J]. 环境科学与技术, 2019,42(5):94-100. Zhao T, Jiang X, Chen Y D, et al. Study on anaerobic co-digestion of excess sludge and citrus pulp[J]. Environmental Science & Technology, 2019,42(5):94-100.
[6]
Cheng H, Li Y, Guo G, et al.Advanced methanogenic performance and fouling mechanism investigation of a high-solid anaerobic membrane bioreactor (AnMBR) for the co-digestion of food waste and sewage sludge[J]. Water Research, 2020,187:116436.
[7]
Wu Y, Song K. Anaerobic co-digestion of waste activated sludge and fish waste:Methane production performance and mechanism analysis[J]. Journal of Cleaner Production, 2021,279:123678.
[8]
Begoña Ruiz, Xavier Flotats. Effect of limonene on batch anaerobic digestion of citrus peel waste[J]. Biochemical Engineering Journal, 2016,109(1):9-18.
[9]
Demetrio A Zema, Adele Fòlino, Giovanni Zappia, et al. Anaerobic digestion of orange peel in a semi-continuous pilot plant:An environmentally sound way of citrus waste management in agro- ecosystems[J]. Science of the Total Environment, 2018,630:401-408.
[10]
陈刚,陈思远,肖向哲,等.橙皮精油对橙渣与剩余污泥厌氧共消化的毒性分析[J]. 中国给水排水, 2021,37(13):77-82. Chen G, Chen S Y, Xiao X Z, et al. Toxicity of orange peel essential oil to anaerobic co-digestion of orange residue and excess sludge[J]. China Water & Wastewater, 2021,37(13):77-82.
[11]
王佳宇,胡文忠,管玉格,等.柠檬烯抑菌机理及其在果蔬保鲜中应用的研究进展[J]. 食品工业科技, 2021,42(14):414-419. Wang J Y, Hu W Z, Guan Y G, et al. Research progress on the bacteriostatic mechanism of limonene and its application in fruit and vegetable preservation[J]. Science and Technology of Food Industry, 2021,42(14):414−419.
[12]
陈思远,陈刚,高彩琪,等.BES对污泥厌氧发酵产酸性能的影响[J]. 江西理工大学学报, 2020,41(1):50-56. Chen S Y, Chen G, Gao C Q, et al. Effects of BES on acid production by anaerobic fermentation of sludge[J]. Journal of Jiangxi University of Science and Technology, 2020,41(1):50-56.
[13]
国家环境保护总局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2004. State Environmental Protection Administration. Water and wastewater monitoring and analysis methods[M]. Beijing:China Environmental Science Press, 2004.
[14]
赵其阳,焦必宁,付陈梅,等.柑桔皮及其果汁中香精油含量的蒸馏滴定方法研究[J]. 中国南方果树, 2006,35(5):9-11. Zhao Q Y, Jiao B N, Fu C M, et al. Rapid determination of the essential oil content in peel and juices of citrus[J]. South China Fruits, 2006,35(5):9-11.
[15]
任晓鸣,陆烽,王蔚.利用气相色谱测定剩余污泥厌氧消化产生的混合VFA[J]. 环境科技, 2009,22(S1):39-41. Ren X M, Lu F, Wang W. Determination of VFAs in anaerobic digestion of excess active sludge by gas chromatography[J]. Environmental Science and Technology, 2009,22(S1):39-41.
[16]
Mahdi Amer Ali, Al-Maqtari Qais Ali, Mohammed Jalaleldeen Khaleel, et al. Enhancement of antioxidant activity, antifungal activity, and oxidation stability of Citrus reticulata essential oil nanocapsules by clove and cinnamon essential oils[J]. Food Bioscience, 2021,43:101226.
[17]
Hema Rughoonundun, Romeela Mohee, Mark T. Influence of carbon- to-nitrogen ratio on the mixed-acid fermentation of wastewater sludge and pretreated bagasse[J]. Bioresource Technology, 2012,112:91-97.
[18]
Xue S R, Wang Y B, Lv X, et al. Interactive effects of carbohydrate, lipid, protein composition and carbon/nitrogen ratio on biogas production of different food wastes[J]. Bioresource Technology, 2020, 312:123566.
[19]
Eryildiz Bahriye, Lukitawesa Lukitawesa, Taherzadeh J Mohammad. Effect of pH, substrate loading, oxygen, and methanogens inhibitors on volatile fatty acid (VFA) production from citrus waste by anaerobic digestion[J]. Bioresource Technology, 2020,302:10.1016/j.biortech. 2020.122800.
[20]
A Carvalho, R Fragoso, J Gominho, et al. Effect of minimizing d-limonene compound on anaerobic co-digestion feeding mixtures to improve methane yield[J]. Waste and Biomass Valorization, 2019, 10(1):75-83.
[21]
Sara Burt. Essential oils:their antibacterial properties and potential applications in foods-a review[J]. International journal of food microbiology, 2004,94(3):223-253.
[22]
Zhao J, Gui L, Wang Q, et al. Aged refuse enhances anaerobic digestion of waste activated sludge[J]. Water Research, 2017,123:724-733.
[23]
Rosangela Di Pasqua, Nikki Hoskins, Gail Betts, et al. Changes in membrane fatty acids composition of microbial cells by addition of thymol, carvacrol, limonen, cinnamaldhehyde, and eugenol in growing media[J]. Journal of Agricultural & Food Chemistry, 2006,54(7):2745-2749.
[24]
Di Pasqua Rosangela, Betts Gail, et al. Membrane toxicity of antimicrobial compounds from essential oils.[J]. Journal of Agricultural and Food Chemistry, 2007,55(12):4863-4870.
[25]
Wikandari Rachma, Nguyen Huong, Millati Ria, et al. Improvement of biogas production from orange peel waste by leaching of limonene[J]. BioMed Research International, 2015,2015:10.1155/2015/494182.
[26]
徐思.杭州西湖沉积物中产甲烷菌群落结构和多样性研究[D]. 武汉:武汉理工大学, 2015. Xu S. Study of methanogenic community structure and diversity in the sediments of West Lake, Hangzhou[D]. Wuhan:Wuhan University of Technology, 2015.
[27]
李小兰,周芸,陈志燕,等.针铁矿强化剩余污泥与烟草废弃物厌氧发酵的研究[J]. 中国环境科学, 2019,39(6):2475-2482. Li X L, Zhou Y, Chen Z Y, et al. Enhanced anaerobic fermentation of waste activated sludge and tobacco waste using Goethite[J]. China Environmental Science, 2019,39(6):2475-2482.
[28]
Wang Y, Wang D, Liu Y, et al. Triclocarban enhances short-chain fatty acids production from anaerobic fermentation of waste activated sludge[J]. Water Research, 2017,127:150-161.
[29]
Li J, Chen T, Yin J, et al. Effect of nano-magnetite on the propionic acid degradation in anaerobic digestion system with acclimated sludge[J]. Bioresource Technology, 2021,334:125143.
[30]
Qing L W, Wan Q G, Zheng H S, et al. Enhancement of volatile fatty acid production by co-fermentation of food waste and excess sludge without pH control:The mechanism and microbial community analyses[J]. Bioresource Technology, 2016,216:653-660.
