Abstract：An integrated PN/A denitrification treatment system was constructed with a mixture of anammox granular sludge and floc sludge. Efficient denitrification of sludge digestion supernatant pretreated with thermal hydrolysis was achieved through the optimization of operating parameters. By inoculating AnAOB biofilm sludge and ordinary activated sludge simultaneously and keeping a high FA (>20mg/L) and low aeration (DO£0.2mg/L), the partial nitrification and anammox reaction was rapidly initiated, and the nitrous accumulation rate got over 85% while the denitrification load reached 0.60kgN/(m3·d). The diluted digestion supernatant still had a certain inhibition on AnAOB activity, resulting in the total nitrogen load of the reactor decreased to less than 0.20kgN/(m3·d). However, the abundance of AnAOB in the system exhibited an overall increasing trend, indicating that the proliferation of AnAOB was not completely restrained. The average median diameter of the sludge mixture in the system increased slowly from 53μm to 109μm. Results of quantitative PCR and high-throughput sequencing showed that the treatment system enriched the AnAOB with a higher purity, the maximum abundance ratio could reach 8.06% with the dominant genus being Kuenenia. In addition, in the 93rd run cycle, the abundance ratio of Kuenenia in granular sludge was higher than that of AOB, which was 5.26%; while in the floc sludge, the abundance of Nitrosomonas for nitrification accounted for 1.64%, higher than that of AnAOB. This demonstrated the different spatial distribution characteristics of AOB and AnAOB abundance.
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