光合细菌对活性污泥微生物群落结构及功能的影响

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摘要构建光序批式反应器(PSBR)处理模拟生活污水,考察光合细菌投加量、pH值、光照强度和溶解氧(DO)对反应器污染物去除性能影响,通过16S rRNA测序技术揭示光合细菌对活性污泥微生物群落结构、功能和氮代谢通路的影响.结果表明:10%光合细菌投加通过促进微生物种间协调功能使PSBR系统COD_Cr、NH₄⁺-N和TN去除率提高,pH值、光照强度和DO分别为7、5000lux和3mg/L时,PSBR脱氮性能最好.光合细菌提高了活性污泥微生物多样性和丰富度,Proteobacteria菌门和norank_f__Saprospiraceae菌属相对丰度增多.光合细菌促进部分参与硝化过程和反硝化过程的功能基因(amo、hao和nap)和酶(AMO、HAO和NAP)丰度增加,活性污泥的整体氮代谢潜力得到提升.

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	杨文焕
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	王志超
	李卫平

关键词 ：光合细菌, 光序批式反应器, 群落功能, 氮代谢

Abstract：The phototrophic bacteria were introduced into the Photoheterotrophic Sequencing Batch Reactor (PSBR) to treat simulated domestic wastewater. The effects of the amount of phototrophic bacteria, pH, light intensity, and dissolved oxygen (DO) on the pollutant removal performance of the reactor were investigated, and the impact of phototrophic bacteria on the microbial community structure, function, and nitrogen metabolism pathway of the active sludge microorganisms was revealed through 16S rRNA sequencing technology. The results showed that the addition of 10% phototrophic bacteria improved the removal rates of COD_Cr, NH₄⁺-N, and TN in the PSBR system by promoting the coordinated functions between microorganisms. The optimum pH, light intensity, and DO for nitrogen removal performance in the PSBR were determined to be 7, 5000lux, and 3mg/L, respectively. The introduction of phototrophic bacteria enhanced the microbial diversity and abundance in the active sludge, with an increase in the relative abundance of Proteobacteria phylum and norank_f__Saprospiraceae genus. The abundance of functional genes (amo, hao, and nap) and enzymes (AMO, HAO, and NAP) involved in partial nitrification and denitrification processes was increased by phototrophic bacteria, thereby enhancing the overall nitrogen metabolism potential of the active sludge.

Key words： photosynthetic bacteria photo sequential batch reactor community function nitrogen metabolism

收稿日期: 2023-07-18

PACS:

X53

基金资助:国家自然科学基金资助项目(42167018)

作者简介: 杨文焕(1980-),女,内蒙古包头人,教授,博士,主要从事水生态修复及保护与水处理技术.发表论文20余篇.1530518003@qq.com.

引用本文:

杨文焕, 邓子威, 徐岩, 王志超, 李卫平. 光合细菌对活性污泥微生物群落结构及功能的影响[J]. 中国环境科学, 2024, 44(3): 1314-1323. YANG Wen-huan, DENG Zi-wei, XU Yan, WANG Zhi-chao, LI Wei-ping. Study on the effect of photosynthetic bacteria on the structure and function of microbial communities in activated sludge systems. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(3): 1314-1323.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I3/1314

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