掺氮碳量子点对光合细菌生长过程的影响

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摘要以柠檬酸为碳源、乙二胺为修饰剂，一步水热合成了掺氮碳量子点（N-CQDs）.通过分析光合色素、蛋白质和丙二醛等生理指标，研究了N-CQDs对光合细菌Rhodopseudomonas acidophila生长的影响.结果表明N-CQDs抑制了R.acidophila的生长并呈现浓度-效应关系；光合色素分析表明N-CQDs促使R.acidophila菌体的类胡萝卜素含量提高，菌绿素含量降低；光谱分析表明N-CQDs导致了胞内物质（如光合色素、蛋白质等）的泄漏.N-CQDs较强的光诱导电子转移能力导致R.acidophila培养体系中产生过量自由基，自由基引发一系列脂质过氧化反应，进而导致生物膜的破裂、物质外泄和细菌衰亡.光照下N-CQDs对R.acidophila表现出较高的毒性.研究结果对于认识N-CQDs的光致毒性及其生态环境效应具有一定的参考价值.

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	周岳陵
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	王进

关键词 ：掺氮碳量子点, 光合细菌, 光合色素, 细胞毒性

Abstract：Nitrogen-doped carbon quantum dots (N-CQDs) were formed by one-step hydrothermal synthesis, using citric acid as carbon source and ethylene diamine as modifier in this study. The effects of N-CQDs on the growth of photosynthetic bacteria Rhodopseudomonas acidophila were investigated by analyzing the physiological indexes of photosynthetic pigment, protein and malondialdehyde. The results showed that N-CQDs inhibited the growth of R. acidophila and showed a concentration-effect relationship. Additionally, N-CQDs increased the content of carotenoid in R. acidophila while it decreased the content of bacteriochlorophyll. Furthermore, spectra analysis results showed that N-CQDs led to the leakage of intracellular sbustances such as photosynthetic pigment and protein. The strong light-induced electron transfer ability of N-CQDs resulted in excessive free radicals in the culture system of R. acidophila, which led to a series of lipid peroxidation reactions, which in turn led to the rupture of biofilms, material leakage and bacterial death. N-CQDs had a high toxicity on R. acidophila under light. The results of this study are valuable for understanding the phototoxicity and ecological effects of N-CQDs.

Key words： N-CQDs photosynthetic bacteria photosynthetic pigment cytotoxicity

收稿日期: 2019-02-20

PACS:

X171.5

基金资助:国家自然科学基金资助项目（41772361）；中央高校基本科研项目（JZ2017YYPY0246）

通讯作者: 王进,教授,sophiawj@hfut.edu.cn E-mail: sophiawj@hfut.edu.cn

作者简介: 周岳陵(1994-),男,湖南衡阳人,硕士研究生,主要研究方向为环境生态修复.发表论文1篇.

引用本文:

周岳陵, 岳正波, 胡馥鹏, 王进. 掺氮碳量子点对光合细菌生长过程的影响[J]. 中国环境科学, 2019, 39(8): 3396-3403. ZHOU Yue-ling, YUE Zheng-bo, HU Fu-peng, WANG Jin. Effect of nitrogen-doped carbon quantum dots on the growth of photosynthetic bacteria. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(8): 3396-3403.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2019/V39/I8/3396

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