Characterization for nitrogen metabolism of sediments in highland saline lake
YANG Wen-huan1, ZHEN Yu1, YAO Zhi1, YIN Qiang2, HUANG Xiao-hui2, LI Wei-ping1
1. College of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China; 2. Inner Mongolia Autonomous Region Enterprises Key Laboratory of Damaged Environment Assessment and Restoration, Hohhot 010010, China
Abstract：In order to explore the adaptation strategy of microbiota in saline-alkali lakes under high salt stress, the community structure and functional characteristics of nitrogen-metabolizing microorganisms in Daihai sediments located in Inner Mongolia were revealed by metagenomic sequencing technology. The effects of environmental factors on nitrogen metabolism and the driving mechanism of microbial community construction under salt stress were explored. The results showed that the nitrogen metabolism of sediments was mainly through nitrate dissimilatory reduction, accompanied by five pathways, including nitrogen fixation, nitrification, denitrification, nitrate assimilatory reduction, and complete ammonia oxidation. Proteobacteria, Chloroflexi, and Actinobacteria were the top three dominant phyla in terms of nitrogen metabolism, which accounted for 56.31%~59.19%, 8.26%~12.89% and 3.2%~6.4% of the total microbial community, respectively. There was no significant difference between this microbiota and other sediment of lakes, but the specificity was significant at the genus level. Thiobacillus, unclassified_c_Gammaproteobacteria, and Thiobalkalivibrio were the dominant genera involved in nitrogen metabolism, which accounted for 9.8%~15.22%, 3.49%~4.02% and 3.49%~4.02% of the total microbial community, respectively. Salinity, total nitrogen, total phosphorus, and pH were the main environmental factors affecting the abundance of the gene. The assembly of the nitrogen metabolism microbial community was driven by deterministic processes, and the microbial community composition of each of the six pathways was distinctive under the effect of environmental filtering. Halophilic methane-oxidizing bacteria and nitrate-reducing sulfur-oxidizing bacteria dominated part of the nitrogen metabolism process in salt-stressed, which coupled the carbon, nitrogen, and sulfur cycles, resulting in the particularity of the nitrogen metabolism process in sediments of Dai Lake.
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