Study on the effect of biochar addition on ammonia emission from constructed wetlands —— Based on the analysis of microbial community assembly mechanism
1. College of Geography and Ocean Sciences, Yanbian University, Yanji 133000, China; 2. State Key Laboratory of Black Soils Conservation and Utilization, Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; 3. University of Chinese Academy of Sciences, Beijing 100049, China
Abstract：This study investigated the impact and mechanisms of different biochar ratios on the denitrification performance and ammonia emission reduction in surface flow constructed wetlands based on mesocosm simulations. Employing machine learning and “infer Community Assembly Mechanisms by Phylogenetic bin-based null model ” (iCAMP), we further explored the microbiological mechanisms involved. The results indicated that, compared to the control group without biochar, a 50% biochar addition significantly promoted the growth of ammonia-oxidizing bacteria (e.g., Chloroflexi, Rhodocyclaceae), enhanced NH4+-N transformation in the system, and effectively reduced ammonia emissions by 32%. Sensitive microbial species identified by the random forest model, such as Planctomycetes and Parcubacteria, could serve as preliminary indicators for ammonia volatilization flux. The proportion of biochar in constructed wetlands was closely linked to changes in community structure. An increase in biochar ratio led to a clustering of phylogenetic structures and promoted the growth and establishment of specific bacterial communities (e.g., Gammaproteobacteria, Deltaproteobacteria) related to carbon and nitrogen cycling. This study offers a new perspective on understanding the factors driving microbial communities in constructed wetlands and provides guidance for the practical application of biochar in these ecosystems.
苗雅琴, 朱卫红, 沈昱廷, 祝惠. 生物炭添加对人工湿地氨气排放的影响研究——基于微生物群落组装机制分析[J]. 中国环境科学, 2024, 44(5): 2514-2525.
MIAO Ya-qin, ZHU Wei-hong, SHEN Yu-ting, ZHU Hui. Study on the effect of biochar addition on ammonia emission from constructed wetlands —— Based on the analysis of microbial community assembly mechanism. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(5): 2514-2525.
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