Effect and mechanism of sulfamethoxazole/trimethoprim on nitrogen removal by solid-phase denitrification
ZHANG Yan-jie1,2,3, WANG Huan1,3, DONG Wei-yang1,3, YAN Guo-kai1,3, CHANG Yang1,3, WANG Hai-yan1,3, LING Yu1,2,3, WEI You-fang1,3, TIAN Zi-yang1,3
1. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 10012, China; 2. College of Water Sciences, Beijing Normal University, Beijing 100875, China; 3. Research Center of Environmental Pollution Control Technology, Chinese Research Academy of Environmental Sciences, Beijing 10012, China
Abstract：It is unclear whether the antibiotics (i.e. sulfamethoxazole (SMX) and trimethoprim (TMP)) in the tail water of wastewater treatment plants would affect the nitrogen removal performance of plant carbon source supported solid-phase denitrification (SPD) technology and its mechanism. Three continuous-flow corncob-SPD (CC-SPD) reactors were established, the nitrogen removal performance of the reactors was compared, and the influence mechanism was analyzed by the metagenomic method. The results showed that: (1) The average effluent NO3--N concentrations of the three reactors were (4.09±0.38) mg/L(RS) (5.01±0.44) mg/L(RT) and (3.53±0.35) mg/L(RC), respectively. 50μg/L SMX/TMP had no significant effect on the nitrogen removal performance of the CC-SPD reactor; (2) SMX/TMP could significantly change the microbial community structure, but the dominant bacteria in the reactor were Proteobacteria, Bacteroidetes, and Actinobacteria. Metabolism was the main function of the microbial community, and microorganisms improved the relative abundances of functions about environmental information processing and cellular processes to adapt to SMX/TMP. 50μg/L SMX/TMP did not significantly affect the relative abundances of enzymes related to electron donor generation and denitrification genes.
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