A<sup>2</sup>O缺氧池添加天然碳源玉米芯的脱氮特征

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (942 KB) HTML (0 KB)
输出: BibTeX | EndNote (RIS)

摘要基于低碳源污水易硝化难反硝化的问题,构建了在A²O缺氧池添加天然碳源玉米芯的中试系统,采用物料衡算、反硝化速率测定和微生物群落分析等方法,研究了该系统的脱氮效能和反硝化体系特征.结果表明,TN去除率提升13%,出水从16.2降至10.0mg/L;同时不会造成出水氨氮和色度超标的风险.物料衡算表明,COD碳源的氧化消耗量和出水排放量降低,更多的碳源用于反硝化和污泥增殖,从而提升了氮素的去除量,其中反硝化的提升贡献更大.缺氧池形成了悬浮污泥加生物膜的复合型脱氮体系:在污水自身碳源存在时,生物膜和悬浮污泥的反硝化速率分别为24.89和32.42mg/(L∙h),可实现快速脱氮;当自身碳源消耗殆尽,二者的反硝化速率分别是4.71和1.73mg/(L×h),单位生物量反硝化速率分别是1.58和59.1mg NO₃^--N/(g VSS×h),表明玉米芯主要被生物膜利用以维持反硝化进行.该体系的主要反硝化菌属为Azospira,此外在生物膜表面还富集了能够附着生长的Iamia和Haliangium,以及能够降解玉米芯木质素的Sulfuritalea等反硝化菌属.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	沈秋实
	吉芳英
	魏嘉志
	姜蕾
	张倩
	毛圆翔
	刘草葱

关键词 ：缺氧池, 玉米芯, 反硝化, 物料衡算, 生物群落

Abstract：Given that low carbon source sewage has advantage of nitrification rather than denitrification, natural carbon source corncob was added into anoxic zone of a pilot A²O to explore the denitrification potency and characteristics, by means of mass balance, denitrification rate and microbial community analysis. The results showed that TN removal efficiency increased by 13%, and effluent TN decreased from 16.2 to 10.0mg/L. Meanwhile, there was no exceeding risk of ammonia nitrogen and chroma in effluent. Mass balance demonstrated that the consumption amount of oxidation and effluent residual of COD both decreased, while its utilization rates for denitrification and sludge proliferation increased, which stimulated nitrogen removal amount. Therein, nitrogen removal was mainly contributed by denitrification. An integrated denitrification system was established in this reactor: the denitrification rates of newly formed biofilm and suspended sludge were 24.89 and 32.42mg/(L×h) separately in the presence of influent carbon source, which achieved this system with rapid denitrification; while influent carbon source was depleted, the denitrification rates were 4.71 and 1.73mg/(L×h) respectively, and their denitrification rates per biomass amount were 1.58 and 59.1mg NO₃^--N/(g VSS×h) accordingly, which indicated that corncob was primarily used by biofilm for maintaining denitrification process. The most dominant denitrifier in this system was Azospira. Morover, denitrifiers like Iamia and Haliangium, capable of adhering to growth, enriched on the biofilm, and Sulfuritalea, which could degrade corncob’s lignin, assembled on the biofilm as well.

Key words： anoxic zone corncob denitrification mass balance microbial community

收稿日期: 2021-09-06

PACS:

X703

基金资助:国家重点研发计划(2018YFD1100501);重庆市科委项目(cstc2017shmsA20007)

通讯作者: 吉芳英,教授,jfy@cqu.edu.cn E-mail: jfy@cqu.edu.cn

作者简介: 沈秋实(1993-),男,河南郑州人,重庆大学博士研究生,主要从事水污染控制研究.发表论文3篇.

引用本文:

沈秋实, 吉芳英, 魏嘉志, 姜蕾, 张倩, 毛圆翔, 刘草葱. A²O缺氧池添加天然碳源玉米芯的脱氮特征[J]. 中国环境科学, 2022, 42(4): 1635-1642. SHEN Qiu-shi, JI Fang-ying, WEI Jia-Zhi, JIANG Lei, ZHANG Qian, MAO Yuan-xiang, LIU Cao-cong. Nitrogen removal characteristic of A²O system with natural corncob supplemented into anoxic zone as carbon source. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(4): 1635-1642.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2022/V42/I4/1635

[1]	郑兴灿.城镇污水处理厂一级A标稳定达标技术[M]. 北京:中国建筑工业出版社, 2015. Zheng X C. The Technologies for urban wastewater treatment plants to reach first level a standard stably[M]. Beijing:China Architecture & Building Press, 2015.
[2]	Wang J, Chu L. Biological nitrate removal from water and wastewater by solid-phase denitrification process[J]. Biotechnology Advances, 2016,34(6):1103-1112.
[3]	邵留,徐祖信,金伟,等.农业废物反硝化固体碳源的优选[J]. 中国环境科学, 2011,31(5):748-754. Shao L, Xu Z X, Jin W, et al. Optimization of solid carbon source for denitrification of agriculture wastes[J]. China Environmental Science, 2011,31(5):748-754.
[4]	赵文莉,郝瑞霞,王润众,等.以碱处理玉米芯为碳源去除二级出水中硝酸盐[J]. 中国给水排水, 2016,32(7):107-111. Zhao W L, Hao R X, Wang R Z, et al. Removal of nitrate from secondary effluent by biological denitrification with corncob pretreated by alkali as carbon source[J]. China Water & Wastewater, 2016,32(7):107-111.
[5]	赵文莉,郝瑞霞,王润众,等.复合碳源填料反硝化脱氮及微生物群落特性[J]. 中国环境科学, 2015,35(10):3003-3009. Zhao W L, Hao R X, Wang R Z, et al. Denitrification of composite carbon filler and character of microbial community[J]. China Environmental Science, 2015,35(10):3003-3009.
[6]	赵文莉,郝瑞霞,李斌,等.预处理方法对玉米芯作为反硝化固体碳源的影响[J]. 环境科学, 2014,35(3):987-994. Zhao W L, Hao R X, Li B, et al. Effects of pretreatment methods on corncob as carbon source for denitrification[J]. Environmental Science, 2014,35(3):987-994.
[7]	叶姜瑜,项宏伟,王宗萍,等.固体碳源及生物强化CAST工艺处理低C/N生活污水的效果[J]. 安徽农业科学, 2017,45(19):58-61,65. Ye J Y, Xiang H W, Wang Z P, et al. Effect of solid carbon source and biofortification CAST process on low C/N domestic sewage wastewater[J]. Journal of Anhui Agricultural Science, 2017,45(19):58-61,65.
[8]	项宏伟.生物强化与固体碳源结合处理小城镇低碳源污水及其微生物群落动态变化分析[D]. 重庆:重庆大学, 2017. Xiang H W. Study on the treatment of low carbon source wastewater and its microbial community dynamics in small towns by bioaugmentation and solid carbon sources[D]. Chongqing:Chongqing University, 2017.
[9]	姜建华.固体碳源及好氧反硝化菌对提高低C/N生活污水脱氮性能的研究[D]. 重庆:重庆大学, 2016. Jiang J H. Using aerobic denitrifiers and solid carbon source to enhance the nitrogen removal performance of low C/N ratio domestic sewage treatment[D]. Chongqing:Chongqing University, 2016.
[10]	Huang L, Ye J, Xiang H, et al. Enhanced nitrogen removal from low C/N wastewater using biodegradable and inert carriers:Performance and microbial shift[J]. Bioresource Technology, 2020,300.
[11]	唐婧,刘昱迪,孙凤海,等.以玉米芯为外加碳源的SBBR脱氮特性[J]. 环境工程学报, 2016,10(6):2775-2780. Tang J, Liu Y D, Sun F H, et al. Characteristics of nitrogen removal in SBBR using corncob as carbon source[J]. Chinese Journal of Environmental Engineering, 2016,10(6):2775-2780.
[12]	张立秋,王登敏,李淑更,等.固体碳源生物膜SND处理实际低碳源城市污水[J]. 工业水处理, 2019,39(8):19-22,106. Zhang L Q, Wang D M, Li S G, et al. Treatment of actual low carbon source urban sewage with solid carbon source biofilm SND[J]. Industrial Water Treatment, 2019,39(8):19-22,106.
[13]	张立秋,王登敏,李淑更,等.水力负荷和C/N比对玉米芯固体碳源SND处理低碳城市污水的影响[J]. 广州大学学报(自然科学版), 2018,17(3):69-75. Zhang L Q, Wang D M, Li S G, et al. Effect of hydraulic load and C/N ratio on corn stalk solid carbon source SND treatment in low-city urban sewage[J]. Journal of Guangzhou University (Natural Science Edition), 2018,17(3):69-75.
[14]	王社平,王卿卿,惠灵灵,等.分段进水A/O脱氮工艺反硝化速率的测定[J]. 环境工程, 2008,(3):56-58,4. Wang S P, Wang Q Q, Hui L L, et al. The determination of denitrification rate of step-feed A/O nitrogen removal process[J]. Environmental Engineering, 2008,(3):56-58,4.
[15]	GB18918-2002城镇污水处理厂污染物排放标准[S]. GB18918-2002 Discharge standard of pollutants for municipal wastewater treatment plants[S].
[16]	吉芳英,张千,徐璇,等.2种BDPs固相反硝化的脱氮效果对比[J]. 环境科学研究, 2014,27(9):1080-1086. Ji F Y, Zhang Q, Xu X, et al. Comparison of two biodegradable polymers in SPD system for nitrogen removal[J]. Research of Environmental Sciences, 2014,27(9):1080-1086.
[17]	Boley A, Muller W R, Haider G. Biodegradable polymers as solid substrate and biofilm carrier for denitrification in recirculated aquaculture systems[J]. Aquacultural Engineering, 2000,22(1/2):75- 85.
[18]	Srinandan C S, Shah M, Patel B, et al. Assessment of denitrifying bacterial composition in activated sludge[J]. Bioresource Technology, 2011,102(20):9481-9489.
[19]	Khan S T, Horiba Y, Yamamoto M, et al. Members of the family Comamonadaceae as primary poly(3-hydroxybutyrate-co-3- hydroxyvalerate)-degrading denitrifiers in activated sludge as revealed by a polyphasic approach[J]. Applied and Environmental Microbiology, 2002,68(7):3206-3214.
[20]	Nittami T, Kasakura R, Kobayashi T, et al. Exploring the operating factors controlling Kouleothrix (type 1851), the dominant filamentous bacterial population, in a full-scale A2O plant[J]. Scientific Reports, 2020,10(1):6809.
[21]	Yang X, Peng Y Z, Ren N Q, et al. Nutrient removal performance and microbial community structure in an EBPR system under the limited filamentous bulking state[J]. Bioresource Technology, 2013,144:86-93.
[22]	Cotto I, Dai Z, Huo L, et al. Long solids retention times and attached growth phase favor prevalence of comammox bacteria in nitrogen removal systems[J]. Water Research, 2020,169.
[23]	Kurahashi M, Fukunaga Y, Sakiyama Y, et al. lamia majanohamensis gen. nov., sp nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of lamiaceae fam. nov[J]. International Journal of Systematic and Evolutionary Microbiology, 2009,59:869-873.
[24]	Jordan D C. Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria from leguminous plants[J]. International Journal of Systematic Bacteriology, 1982,32(1):136-139.
[25]	Purkhold U, Pommerening-Roser A, Juretschko S, et al. Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis:Implications for molecular diversity surveys[J]. Applied and Environmental Microbiology, 2000, 66(12):5368-5382.
[26]	Nielsen P H, de Muro M A, Nielsen J L. Studies on the in situ physiology of Thiothrix spp. present in activated sludge[J]. Environmental Microbiology, 2000,2(4):389-398.
[27]	Fudou R, Jojima Y, Iizuka T, et al. Haliangium ochraceum gen. nov., sp nov and Haliangium tepidum sp nov.:Novel moderately halophilic myxobacteria isolated from coastal saline environments[J]. Journal of General and Applied Microbiology, 2002,48(2):109-115.
[28]	Sperfeld M, Diekert G, Studenik S. Anaerobic aromatic compound degradation in sulfuritalea hydrogenivorans sk43H[J]. FEMS Microbiology Ecology, 2019,95(1):fiy199.
[29]	Lim J H, Baek S-H, Lee S-T. Ferruginibacter alkalilentus gen. nov., sp nov and Ferruginibacter lapsinanis sp nov., novel members of the family 'Chitinophagaceae' in the Phylum Bacteroidetes, isolated from freshwater sediment[J]. International Journal of Systematic and Evolutionary Microbiology, 2009,59:2394-2399.
[30]	McBride M J, Xie G, Martens E C, et al. Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis[J]. Applied and Environmental Microbiology, 2009,75(21):6864-6875.