金属纳米材料对细菌耐药的影响及其机制研究进展

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摘要抗生素耐药性是21世纪人类面临的最严峻的公共健康问题之一.金属纳米材料因其优良的生物杀灭能力和可调节特性,被视为后抗生素时代应对细菌耐药的有效武器.然而,近年来有研究表明,细菌不仅对纳米材料本身可产生抗性,同时这些纳米材料反过来也会影响细菌的生理特性,导致其对抗生素的耐药性增加.综述了金属纳米材料暴露引起的细菌对抗生素耐药性的变化,并从纳米材料与细菌细胞之间的相互作用、细菌基因突变和抗性基因的传播扩散等方面探讨其作用机制.旨在为开发新型纳米抗菌剂提供理论基础,促进纳米材料在抗菌领域的应用,以应对全球耐药性挑战.

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关键词 ：纳米材料, 抗生素耐药性, 基因突变, 活性氧, 水平基因转移

Abstract：Antibiotic resistance was recognized as one of the most critical public health challenges confronted by humanity in the 21 st century. Metal nanomaterials were regarded as potent alternatives in the post-antibiotic era, attributed to their exceptional biocidal efficacy and tunable properties. However, it was demonstrated through recent studies that not only could resistance to nanomaterials themselves be developed by bacteria, but the physiological characteristics of bacteria could also be altered, consequently leading to enhanced antibiotic resistance. The antibiotic resistance variations induced by metal nanomaterials were systematically reviewed, with underlying mechanisms being elucidated through three key aspects: the interfacial interactions between nanomaterials and bacterial membranes, the occurrence of bacterial genomic mutations, and the horizontal transfer of resistance genes. This investigation was designed to establish a theoretical framework for innovating next-generation nano-antimicrobial agents, while simultaneously promoting the application of nanomaterials in combating antimicrobial resistance on a global scale.

Key words： nanomaterials antibiotic resistance gene mutation reactive oxygen species horizontal gene transfer

收稿日期: 2024-09-26

PACS:

X172

基金资助:国家自然科学基金项目(22076167,U21A20292);浙江省大学生科技创新活动计划(1260KZN0224073G)

作者简介: 龙琳(2000-),女,湖南怀化人,浙江工商大学硕士研究生,主要从事环境微生物方向的研究工作.longlin121211@163.com.

引用本文:

龙琳, 朱琳, 汤惠茗, 汪美贞. 金属纳米材料对细菌耐药的影响及其机制研究进展[J]. 中国环境科学, 2025, 45(5): 2857-2864. LONG Lin, ZHU Lin, TANG Hui-ming, WANG Mei-zhen. Research progress on the effect of metallic nanomaterials on the resistance of bacteria and its mechanism. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(5): 2857-2864.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2025/V45/I5/2857

[1] Zhang C, Sun R, Xia T. Adaption/resistance to antimicrobial nanoparticles: Will it be a problem?[J]. Nano Today, 2020,34:100909.
[2] Makvandi P, Wang C Y, Zare E N, et al. Metal-based nanomaterials in biomedical applications: antimicrobial activity and cytotoxicity aspects[J]. Advanced Functional Materials, 2020,30(22):1910021.
[3] Franco D, Calabrese G, Guglielmino S P P, et al. Metal-based nanoparticles: antibacterial mechanisms and biomedical application[J]. Microorganisms, 2022,10(9):1778.
[4] Du J, Zhang Y, Yin Y, et al. Do environmental concentrations of zinc oxide nanoparticle pose ecotoxicological risk to aquatic fungi associated with leaf litter decomposition?[J]. Water Research, 2020, 178:115840.
[5] Yin L, Cheng Y, Espinasse B, et al. More than the ions: the effects of silver nanoparticles on Lolium multiflorum[J]. Environmental Science& Technology, 2011,45(6):2360-2367.
[6] Qiu Z, Yu Y, Chen Z, et al. Nanoalumina promotes the horizontal transfer of multiresistance genes mediated by plasmids across genera[J]. Proceedings of the National Academy of Sciences, 2012,109(13): 4944-4949.
[7] Giese B, Klaessig F, Park B, et al. Risks, Release and concentrations of engineered nanomaterial in the environment[J]. Scientific Reports, 2018,8(1):1565.
[8] Yin Y, Yu S, Liu J, et al. Thermal and photoinduced reduction of ionic Au (III) to elemental Au nanoparticles by dissolved organic matter in water: possible source of naturally occurring Au nanoparticles[J]. Environmental Science& Technology, 2014,48(5):2671-2679.
[9] Bakshi M, Kumar A. Copper-based nanoparticles in the soil-plant environment: assessing their applications, interactions, fate and toxicity[J]. Chemosphere, 2021,281:130940.
[10] Ali T, Warsi M F, Zulfiqar S, et al. Green nickel/nickel oxide nanoparticles for prospective antibacterial and environmental remediation applications[J]. Ceramics International, 2022,48(6): 8331-8340.
[11] Waseem A, Arshad J, Iqbal F, et al. Pollution Status of Pakistan: a retrospective review on heavy metal contamination of water, soil, and vegetables[J]. BioMed Research International, 2014,2014:1-29.
[12] Milla M, Yu S-M, Laromaine A. Parametrizing the exposure of superparamagnetic iron oxide nanoparticles in cell cultures at different in vitro environments[J]. Chemical Engineering Journal, 2018,340:173-180.
[13] Gottschalk F, Sun T, Nowack B. Environmental concentrations of engineered nanomaterials: review of modeling and analytical studies[J]. Environmental Pollution, 2013,181:287-300.
[14] Ding C, Pan J, Jin M, et al. Enhanced uptake of antibiotic resistance genes in the presence of nanoalumina[J]. Nanotoxicology, 2016, 10(8):1051-1060.
[15] Kaweeteerawat C, Na Ubol P, Sangmuang S, et al. Mechanisms of antibiotic resistance in bacteria mediated by silver nanoparticles[J]. Journal of Toxicology and Environmental Health, Part A, 2017, 80(23/24):1276-1289.
[16] Suardiaz R, Lythell E, Hinchliffe P, et al. Catalytic mechanism of the colistin resistance protein MCR-1[J]. Organic& Biomolecular Chemistry, 2021,19(17):3813-3819.
[17] Su Y, Wu D, Xia H, et al. Metallic nanoparticles induced antibiotic resistance genes attenuation of leachate culturable microbiota: the combined roles of growth inhibition, ion dissolution and oxidative stress[J]. Environment International, 2019,128:407-416.
[18] Zhang Y, Hudson-Smith N V, Frand S D, et al. Influence of the spatial distribution of cationic functional groups at nanoparticle surfaces on bacterial viability and membrane interactions[J]. Journal of the American Chemical Society, 2020,142(24):10814-10823.
[19] Zhou Z, Lian Y, Zhu L, et al. Platinum nanoparticles prevent the resistance of pseudomonas aeruginosa to ciprofloxacin and imipenem: mechanism insights[J]. ACS Nano, 2023,17(24):24685-24695.
[20] Li Z, Zhang Y, Huang D, et al. Through quorum sensing, Pseudomonas aeruginosa resists noble metal-based nanomaterials toxicity[J]. Environmental Pollution, 2021,269:116138.
[21] Bhattacharya P, Dey A, Neogi S. An insight into the mechanism of antibacterial activity by magnesium oxide nanoparticles[J]. Journal of Materials Chemistry B, 2021,9(26):5329-5339.
[22] Zhang Y, Gu A Z, Xie S, et al. Nano-metal oxides induce antimicrobial resistance via radical-mediated mutagenesis[J]. Environment International, 2018,121:1162-1171.
[23] Gudkov S V, Burmistrov D E, Serov D A, et al. Do iron oxide nanoparticles have significant antibacterial properties?[J]. Antibiotics, 2021,10(7):884.
[24] Yu Z, Li X, Guo J. Combat antimicrobial resistance emergence and biofilm formation through nanoscale zero-valent iron particles[J]. Chemical Engineering Journal, 2022,444:136569.
[25] Zhang Q, Xia T, Zhang C. Chronic exposure to titanium dioxide nanoparticles induces commensal-to-pathogen transition in Escherichia coli[J]. Environmental Science& Technology, 2020,54(20):13186-13196.
[26] Yang Y, Alvarez P J J. Sublethal concentrations of silver nanoparticles stimulate biofilm development[J]. Environmental Science& Technology Letters, 2015,2(8):221-226.
[27] Ouyang K, Mortimer M, Holden P A, et al. Towards a better understanding of Pseudomonas putida biofilm formation in the presence of ZnO nanoparticles (NPs): Role of NP concentration[J]. Environment International, 2020,137:105485.
[28] Matula K, Richter L, Janczuk-Richter M, et al. Phenotypic plasticity of Escherichia coli upon exposure to physical stress induced by ZnO nanorods[J]. Scientific Reports, 2019,9(1):8575.
[29] Cui J, Zhang H, Mo Z, et al. Cell wall thickness and the molecular mechanism of heterogeneous vancomycin-intermediate Staphylococcus aureus[J]. Letters in Applied Microbiology, 2021,72(5):604-609.
[30] Leifert A, Pan Y, Kinkeldey A, et al. Differential hERG ion channel activity of ultrasmall gold nanoparticles[J]. Proceedings of the National Academy of Sciences, 2013,110(20):8004-8009.
[31] Du W, Sun Y, Ji R, et al. TiO₂O₂and ZnO nanoparticles negatively affect wheat growth and soil enzyme activities in agricultural soil[J]. Journal of Environmental Monitoring, 2011,13(4):822-828.
[32] Hu C, He G, Yang Y, et al. Nanomaterials regulate bacterial quorum sensing: applications, mechanisms, and optimization strategies[J]. Advanced Science, 2024,11(15):2306070.
[33] Li M, Li J, Sun J, et al. Is sulfidation a true detoxification process for silver nanoparticles?: from the perspective of chronic exposure[J]. Environmental Science: Nano, 2019,6(12):3611-3624.
[34] Wang M, Lian Y, Wang Y, et al. The role and mechanism of quorum sensing on environmental antimicrobial resistance[J]. Environmental Pollution, 2023,322:121238.
[35] Alcalde-Rico M, Olivares-Pacheco J, Alvarez-Ortega C, et al. Role of the multidrug resistance efflux pump MexCD-OprJ in the Pseudomonas aeruginosa quorum sensing response[J]. Frontiers in Microbiology, 2018,9:2752.
[36] Lu J, Zhang S, Gao S, et al. New insights of the bacterial response to exposure of differently sized silver nanomaterials[J]. Water Research, 2020,169:115205.
[37] Huang H, Chen Y, Yang S, et al. CuO and ZnO nanoparticles drive the propagation of antibiotic resistance genes during sludge anaerobic digestion: possible role of stimulated signal transduction[J]. Environmental Science: Nano, 2019,6(2):528-539.
[38] Hasani A, Madhi M, Gholizadeh P, et al. Metal nanoparticles and consequences on multi-drug resistant bacteria: reviving their role[J]. SN Applied Sciences, 2019,1(4):1-13.
[39] Ding C, Jin M, Ma J, et al. Nano-Al₂O₃ can mediate transduction-like transformation of antibiotic resistance genes in water[J]. Journal of Hazardous Materials, 2021,405:124224.
[40] Chowdhury N N, Cox A R, Wiesner M R. Nanoparticles as vectors for antibiotic resistance: the association of silica nanoparticles with environmentally relevant extracellular antibiotic resistance genes[J]. Science of the Total Environment, 2021,761:143261.
[41] Wang X, Chen Q, Pang R, et al. Exposure modes determined the effects of nanomaterials on antibiotic resistance genes: The different roles of oxidative stress and quorum sensing[J]. Environmental Pollution, 2024,360:124772.
[42] Xiao X, Ma X L, Han X, et al. TiO₂-photoexcitation promoted horizontal transfer of resistance genes mediated by phage transduction[J]. Science of the Total Environment, 2021,760:144040.
[43] Xie M, Gao M, Yun Y, et al. Antibacterial nanomaterials: mechanisms, impacts on antimicrobial resistance and design principles[J]. Angewandte Chemie International Edition, 2023,62(17):e202217345.
[44] Qiu Z, Shen Z, Qian D, et al. Effects of nano-TiO₂ on antibiotic resistance transfer mediated by RP4plasmid[J]. Nanotoxicology, 2015,9(7):895-904.
[45] Zhang P, Qiu Y, Wang Y, et al. Nanoparticles promote bacterial antibiotic tolerance via inducing hyperosmotic stress response[J]. Small, 2022,18(19):e2105525.
[46] Pu Q, Fan X T, Sun A Q, et al. Co-effect of cadmium and iron oxide nanoparticles on plasmid-mediated conjugative transfer of antibiotic resistance genes[J]. Environment International, 2021,152:106453.
[47] Liu X, Tang J, Song B, et al. Exposure to Al₂O₃ nanoparticles facilitates conjugative transfer of antibiotic resistance genes from Escherichia coli to Streptomyces[J]. Nanotoxicology, 2019,13(10): 1422-1436.
[48] Zhang S, Lu J, Wang Y, et al. Insights of metallic nanoparticles and ions in accelerating the bacterial uptake of antibiotic resistance genes[J]. Journal of Hazardous Materials, 2022,421:126728.
[49] Jin C, Cao J, Zhang K, et al. Promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soil[J]. Ecotoxicology and Environmental Safety, 2023,256:114913.
[50] Yu K, Chen F, Yue L, et al. CeO₂ nanoparticles regulate the propagation of antibiotic resistance genes by altering cellular contact and plasmid transfer[J]. Environmental Science& Technology, 2020, 54(16): 10012-10021.
[51] Jiang Q, Feng M, Ye C, et al. Effects and relevant mechanisms of non-antibiotic factors on the horizontal transfer of antibiotic resistance genes in water environments: a review[J]. Science of the Total Environment, 2022,806:150568.
[52] Marinacci B, Krzyzek P, Pellegrini B, et al. Latest update on outer membrane vesicles and their role in horizontal gene transfer: a mini-review[J]. Membranes, 2023,13(11):860.