土壤中抗生素抗性基因的分布及迁移转化

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摘要

为明晰土壤中抗生素抗性基因的存在及传播状况，并为开展抗生素抗性基因土壤污染的风险评价工作提供科学的理论依据.本文根据抗生素抗性基因存在和转移的理论基础，结合国内外研究进展，详细阐述了土壤中抗生素抗性基因的来源、分布传播及其影响因素，发现已有研究对外源抗生素抗性基因在土壤中的丰度和种类变化的研究较为深入，而对其在土壤中的空间分布特征、传播扩散及行为机理等研究工作尚处于起步阶段.因此提出未来土壤抗生素抗性基因的研究重点应为掌握抗生素抗性基因的空间分布、溯源、迁移转化规律及其关键制约因子，并建立相应的数量关系.

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关键词 ：土壤, 抗生素抗性基因, 分布和传播, 水平基因转移

Abstract：

Soil is considered as one of the primary receivers of antibiotic resistance genes (ARGs). Hence, clarifying the distribution, transfer of ARGs in soil, is very important to evaluate the potential ecological risks of ARGs. In this paper, the origin, distribution and transfer of ARGs in soil were examined systematically, the major environmental factors that influenced the transmission of ARGs were summarized, and the necessity of the ARGs research in soil was also emphasized. Results showed that the previous studies mostly focus on the abundance and variety of exogenous ARGs in soil, while spatial distribution, propagation and behavior mechanism of ARGs are still found to be insufficient. Based on current research, prospective ARGs research in soil should master the distribution, source, rule of transformation and the key restriction factors. Furthermore a corresponding quantitative relationship should be established to provide a scientific basis data for the assessment of ARGs pollution and ecological risk.

Key words： soil antibiotic resistance genes distribution and propagate horizontal gene transfer

收稿日期: 2017-12-22

X53

基金资助:

国家自然科学基金资助项目（51378287）

通讯作者: 刘翔,教授,x.liu@tsinghua.edu.cn E-mail: x.liu@tsinghua.edu.cn

作者简介: 张宁(1983-),女,山东德州人,清华大学博士研究生,主要从事土壤及地下水中抗生素抗性基因分布与传播研究.发表论文8篇.

引用本文:

张宁, 李淼, 刘翔. 土壤中抗生素抗性基因的分布及迁移转化[J]. 中国环境科学, 2018, 38(7): 2609-2617. ZHANG Ning, LI Miao, LIU Xiang. Distribution and transformation of antibiotic resistance genes in Soil. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(7): 2609-2617.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2018/V38/I7/2609

[1]	Cho I, Blaser M J. The human microbiome:at the interface of health and disease[J]. Nature Reviews Genetics, 2012,13(4):260-270.
[2]	Rayner C, Munckhof W J. Antibiotics currently used in the treatment of infections caused by Staphylococcus aureus[J]. Internal Medicine Journal, 2005,35(s2):S3S16.
[3]	Zhan X, Xiao L. Livestock Waste 2016-International conference on recent advances in pollution control and resource recovery for the livestock sector[J]. Frontiers of Environmental Science & Engineering, 2017,11:16.
[4]	Gao P, Mao D, Luo Y, et al. Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment[J]. Water Research, 2012,46(7):2355-2364.
[5]	Levy S B, Marshall B. Antibacterial resistance worldwide:causes, challenges and responses[J]. Nature Medicine, 2004,10(12s):S122-S129.
[6]	Wright G D. Antibiotic resistance in the environment:a link to the clinic?[J]. Current Opinion in Microbiology, 2010,13(5):589-594.
[7]	2013年八国集团首脑会议[EB/OL]. https://www.gov.uk/government/news/g8-science-ministers-statement. 2013-06-12.
[8]	Aminov R I. Horizontal gene exchange in environmental microbiota[J]. Frontiers in Microbiology, 2011,2:158.
[9]	Brown-Jaque M, Calero-Cáceres W, Muniesa M. Transfer of antibiotic-resistance genes via phage-related mobile elements[J]. Plasmid, 2015, 79:1-7.
[10]	Forsberg K J, Reyes A, Wang B, et al. The shared antibiotic resistome of soil bacteria and human pathogens[J]. Science, 2012,337(6098):1107-1111.
[11]	Salyers A A, Amabile-Cuevas C F. Why are antibiotic resistance genes so resistant to elimination?[J]. Antimicrobial Agents and Chemotherapy, 1997,41(11):2321-2325.
[12]	Chee-Sanford J C, Mackie R I, Koike S, et al. Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste[J]. Journal of Environmental Quality, 2009, 38(3):1086-1108.
[13]	Chen C, Li J, Chen P, et al. Occurrence of antibiotics and antibiotic resistances in soils from wastewater irrigation areas in Beijing and Tianjin, China[J]. Environmental Pollution, 2014,193:94-101.
[14]	D'Costa V M, King C E, Kalan L, et al. Antibiotic resistance is ancient[J]. Nature, 2011,477(7365):457-461.
[15]	Allen H K, Moe L A, Rodbumrer J, et al. Functional metagenomics reveals diverse β-lactamases in a remote Alaskan soil[J]. The ISME Journal, 2009,3(2):243-251.
[16]	Chen B, Yuan K, Chen X, et al. Metagenomic analysis revealing antibiotic resistance genes (ARGs) and their genetic compartments in the Tibetan environment[J]. Environmental Science & Technology, 2016,50(13):6670-6679.
[17]	Bhullar K, Waglechner N, Pawlowski A, et al. Antibiotic resistance is prevalent in an isolated cave microbiome[J]. PloS one, 2012,7(4):e34953.
[18]	Stalder T, Barraud O, Jové T, et al. Quantitative and qualitative impact of hospital effluent on dissemination of the integron pool[J]. The ISME Journal, 2014,8(4):768-777.
[19]	Jakobsen L, Sandvang D, Hansen L H, et al. Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment[J]. Environment international, 2008,34(1):108-115.
[20]	李侃竹,高品,王凯,等.污水中抗生素与重金属对红霉素抗药性基因的选择性效应[J]. 中国环境科学, 2015,35(3):889-896.
[21]	Pei R, Kim S C, Carlson K H, et al. Effect of river landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG)[J]. Water Research, 2006,40(12):2427-2435.
[22]	Zhu Y G, Johnson T A, Su J Q, et al. Diverse and abundant antibiotic resistance genes in Chinese swine farms[J]. Proceedings of the National Academy of Sciences, 2013,110(9):3435-3440.
[23]	Sengeløv G, Agersø Y, Halling-Sørensen B, et al. Bacterial antibiotic resistance levels in Danish farmland as a result of treatment with pig manure slurry[J]. Environment International, 2003,28(7):587-595.
[24]	Byrne-Bailey K G, Gaze W H, Kay P, et al. Prevalence of sulfonamide resistance genes in bacterial isolates from manured agricultural soils and pig slurry in the United Kingdom[J]. Antimicrobial Agents and Chemotherapy, 2009,53(2):696-702.
[25]	彭双,王一明,林先贵.连续施用发酵猪粪对土壤中四环素抗性基因数量的影响[J]. 中国环境科学, 2015,35(4):1173-1180.
[26]	张兰河,王佳佳,高敏,等.施用畜禽粪便有机肥土壤抗生素抗性基因污染状况[J]. 生态与农村环境学报, 2016,32(4):664-669.
[27]	王瑞,魏源送.畜禽粪便中残留四环素类抗生素和重金属的污染特征及其控制[J]. 农业环境科学学, 2013,1(9):1705-1719.
[28]	Ding G C, Radl V, Schloter-Hai B, et al. Dynamics of soil bacterial communities in response to repeated application of manure containing sulfadiazine[J]. PLoS One, 2014,9(3):e92958.
[29]	Kopmann C, Jechalke S, Rosendahl I, et al. Abundance and transferability of antibiotic resistance as related to the fate of sulfadiazine in maize rhizosphere and bulk soil[J]. FEMS Microbiology Ecology, 2012,83(1):125-134.
[30]	Jechalke S, Heuer H, Siemens J, et al. Fate and effects of veterinary antibiotics in soil[J]. Trends in Microbiology, 2014,22(9):536-545.
[31]	Heuer H, Schmitt H, Smalla K. Antibiotic resistance gene spread due to manure application on agricultural fields[J]. Current Opinion in Microbiology, 2011,14(3):236-243.
[32]	Negreanu Y, Pasternak Z, Jurkevitch E, et al. Impact of treated wastewater irrigation on antibiotic resistance in agricultural soils[J]. Environmental Science & Technology, 2012,46(9):4800-4808.
[33]	隋倩雯,张俊亚,魏源送,等.畜禽养殖废水生物处理与农田利用过程抗生素抗性基因的转归特征研究进展[J]. 环境科学学报, 2016,36(1):16-26.
[34]	Rahube T O, Yost C K. Antibiotic resistance plasmids in wastewater treatment plants and their possible dissemination into the environment[J]. African Journal of Biotechnology, 2010,9(54):9183-9190.
[35]	van Elsas J D, Bailey M J. The ecology of transfer of mobile genetic elements[J]. FEMS Microbiology Ecology, 2002,42(2):187-197.
[36]	Onan L J, LaPara T M. Tylosin-resistant bacteria cultivated from agricultural soil[J]. FEMS Microbiology Letters, 2003,220(1):15-20.
[37]	Pruden A, Pei R, Storteboom H, et al. Antibiotic resistance genes as emerging contaminants:studies in northern Colorado[J]. Environmental Science & Technology, 2006,40(23):7445-7450.
[38]	Marti R, Scott A, Tien Y C, et al. Impact of manure fertilization on the abundance of antibiotic-resistant bacteria and frequency of detection of antibiotic resistance genes in soil and on vegetables at harvest[J]. Applied and Environmental Microbiology, 2013,79(18):5701-5709.
[39]	Popowska M, Rzeczycka M, Miernik A, et al. Influence of soil use on prevalence of tetracycline, streptomycin, and erythromycin resistance and associated resistance genes[J]. Antimicrobial Agents and Chemotherapy, 2012,56(3):1434-1443.
[40]	Zielezny Y, Groeneweg J, Vereecken H, et al. Impact of sulfadiazine and chlorotetracycline on soil bacterial community structure and respiratory activity[J]. Soil Biology and Biochemistry, 2006,38(8):2372-2380.
[41]	Schmitt H, Stoob K, Hamscher G, et al. Tetracyclines and tetracycline resistance in agricultural soils:microcosm and field studies[J]. Microbial Ecology, 2006,51(3):267-276.
[42]	Knapp C W, Dolfing J, Ehlert P A I, et al. Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940[J]. Environmental Science & Technology, 2009,44(2):580-587.
[43]	张俊,杨晓洪,葛峰,等.长期施用四环素残留猪粪对土壤中耐药菌及抗性基因形成的影响[J]. 环境科学, 2014,35(6):2374-2380.
[44]	Yin Q, Yue D, Peng Y, et al. Occurrence and distribution of antibiotic-resistant bacteria and transfer of resistance genes in Lake Taihu[J]. Microbes and Environments, 2013,28(4):479-486.
[45]	徐晨光.茶园土壤抗生素抗性细菌及抗性基因研究[D]. 杭州:浙江大学, 2014.
[46]	Ochman H, Lawrence J G, Groisman E A. Lateral gene transfer and the nature of bacterial innovation[J]. Nature, 2000,405(6784):299-304.
[47]	徐冰洁,罗义,周启星,等.抗生素抗性基因在环境中的来源,传播扩散及生态风险[J]. 环境化学, 2010,29(2):169-178.
[48]	Dodd M C. Potential impacts of disinfection processes on elimination and deactivation of antibiotic resistance genes during water and wastewater treatment[J]. Journal of Environmental Monitoring, 2012, 14(7):1754-1771.
[49]	刘苗苗,杨敏,张昱,等.环境中抗药基因水平转移研究进展[J]. 生态毒理学报, 2015,(5):11-19.
[50]	Taylor D E, Gibreel A, Tracz D M, et al. Plasmid Biology:Antibiotic resistance plasmids[M]. American Society of Microbiology, 2004:473-492.
[51]	Binh C T T, Heuer H, Kaupenjohann M, et al. Piggery manure used for soil fertilization is a reservoir for transferable antibiotic resistance plasmids[J]. FEMS Microbiology Ecology, 2008,66(1):25-37.
[52]	Musovic S, Dechesne A, Sørensen J, et al. Novel assay to assess permissiveness of a soil microbial community toward receipt of mobile genetic elements[J]. Applied and Environmental Microbiology, 2010, 76(14):4813-4818.
[53]	de Vries J, Wackernagel W. Microbial horizontal gene transfer and the DNA release from transgenic crop plants[J]. Plant and Soil, 2005, 266(1/2):91-104.
[54]	Zhu B. Degradation of plasmid and plant DNA in water microcosms monitored by natural transformation and real-time polymerase chain reaction (PCR)[J]. Water Research, 2006,40(17):3231-3238.
[55]	He L Y, Liu Y S, Su H C, et al. Dissemination of antibiotic resistance genes in representative broiler feedlots environments:identification of indicator ARGs and correlations with environmental variables[J]. Environmental Science & Technology, 2014,48(22):13120-13129.
[56]	Poté J, Ceccherini M T, Rosselli W, et al. Fate and transport of antibiotic resistance genes in saturated soil columns[J]. European Journal of Soil Biology, 2003,39(2):65-71.
[57]	Chen P, Chen C, Li X. Transport of antibiotic resistance plasmids in porous media and the influence of surfactants[J]. Frontiers of Environmental Science & Engineering, 2017,12(2):5.
[58]	Koike S, Krapac I G, Oliver H D, et al. Monitoring and source tracking of tetracycline resistance genes in lagoons and groundwater adjacent to swine production facilities over a 3-year period[J]. Applied and Environmental Microbiology, 2007,73(15):4813-4823.
[59]	Global water and food safety[EB/OL]. http://publish.illinois.edu/helen-nguyen/past-project-antibiotic-resistance. 2012.
[60]	Munir M, Xagoraraki I. Levels of antibiotic resistance genes in manure, biosolids, and fertilized soil[J]. Journal of Environmental Quality, 2011, 40(1):248-255.
[61]	Munir M, Wong K, Xagoraraki I. Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan[J]. Water Research, 2011,45(2):681-693.
[62]	Cai P, Huang Q Y, Zhang X W. Interactions of DNA with clay minerals and soil colloidal particles and protection against degradation by DNase[J]. Environmental Science & Technology, 2006,40(9):2971-2976.
[63]	Saeki K, Kunito T, Sakai M. Effects of pH, ionic strength, and solutes on DNA adsorption by andosols[J]. Biology and fertility of soils, 2010, 46(5):531-535.
[64]	Sørensen S J, Bailey M, Hansen L H, et al. Studying plasmid horizontal transfer in situ:a critical review[J]. Nature Reviews Microbiology, 2005,3(9):700.
[65]	Auerbach E A, Seyfried E E, McMahon K D. Tetracycline resistance genes in activated sludge wastewater treatment plants[J]. Water Research, 2007,41(5):1143-1151.
[66]	Ji X, Shen Q, Liu F, et al. Antibiotic resistance gene abundances associated with antibiotics and heavy metals in animal manures and agricultural soils adjacent to feedlots in Shanghai; China[J]. Journal of Hazardous Materials, 2012,235:178-185.
[67]	Berg J, Thorsen M K, Holm P E, et al. Cu exposure under field conditions coselects for antibiotic resistance as determined by a novel cultivation-independent bacterial community tolerance assay[J]. Environmental Science & Technology, 2010,44(22):8724-8728.
[68]	杨芬.锌对土壤菌群及抗生素抗性水平转移有关基因的影响[D]. 镇江:江苏科技大学, 2016.
[69]	Tumah H N. Bacterial biocide resistance[J]. Journal of Chemotherapy, 2009,21(1):5-15.
[70]	汪庆,罗义,崔玉晓,等.离子液体[BMIm] [PF6] 增强抗性质粒RP4介导的接合转移基因mRNA的表达水平[J]. 生态毒理学报, 2017, 12(3):273-281.
[71]	Trevors J T, Oddie K M. R-plasmid transfer in soil and water[J]. Canadian Journal of Microbiology, 1986,32(7):610-613.
[72]	Guo M T, Yuan Q B, Yang J. Distinguishing effects of ultraviolet exposure and chlorination on the horizontal transfer of antibiotic resistance genes in municipal wastewater[J]. Environmental Science & Technology, 2015,49(9):5771-5778.