Toxic effects of nonylphenol on Selenastrum capricornutum
LIU Wei-jie1, WU Xiao-qing2, YAN Jia-ying1, HE Ning3, CHEN Qi1, DUAN Shun-shan1
1. Research Center of Hydrobiology, Jinan University, Guangzhou 510632, China; 2. South China Institute of Environmental Sciences, MEP, Guangzhou 510655, China; 3. Collage of Life Science and Resources and Environment, Yichun University, Yichun 336000, China
Abstract：In order to investigate the toxic effects of nonyphenol (NP) on fresh microalgae, Selenastrum capricornutum was exposed to NP with concentrations of 0.1, 0.3, 0.6, 0.9, and 1.2mg/L. The growth, chlorophyll content, superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were measured. Besides, chlorophyll fluorescence was obtained through a handy PEA, investigating the influence of NP on the PSⅡ reaction center. The results showed that the 96h EC50 of NP on S. capricornutum was 0.979mg/L. It was found that 0.3mg/L of NP could significantly inhibit the growth of S. capricornutum with decrease in contents of chlorophyll and maximal photochemistry (Fv/Fm), while increase in contents of MDA, activities of SOD and CAT, light absorbed by PSⅡ(ABS/RC), TR0/RC and the dissipation of energy from reaction centers (DI0/RC). More importantly, the cellular membrane system and PSⅡ reaction center of S.capricornutum were damaged obviously when the NP concentrations were higher than 0.9mg/L:the activities of SOD and CAT, and TR0/RC were reduced, while DI0/RC was increased significantly. As a consequent, NP is toxic to S. capricornutum and it may be a potential risk to the aquatic ecosystem.
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