胞外聚合物和信号分子对厌氧氨氧化污泥活性的影响

张亚超; 张晶; 侯爱月; 周荣煊; 梁东博; 张凯; 刘阳; 李军

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中国环境科学 ›› 2019, Vol. 39 ›› Issue (10) : 4133-4140.

水污染与控制

胞外聚合物和信号分子对厌氧氨氧化污泥活性的影响

张亚超¹, 张晶¹, 侯爱月², 周荣煊¹, 梁东博¹, 张凯¹, 刘阳¹, 李军¹

作者信息 +

Effect of extracellular polymers and signal molecules on the activity of ANAMMOX sludge

ZHANG Ya-chao¹, ZHANG Jing¹, HOU Ai-yue², ZHOU Rong-xuan¹, LIANG Dong-bo¹, ZHANG Kai¹, LIU Yang¹, LI Jun¹

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摘要

为了探究胞外聚合物（EPS）对厌氧氨氧化颗粒污泥活性的影响，分别向厌氧氨氧化颗粒污泥中投加30mg/L的总胞外聚合物（IN-EPS），松散结合型胞外聚合物（LB-EPS）和紧密结合型胞外聚合物（TB-EPS）.结果表明，3种EPS均可提高厌氧氨氧化颗粒污泥的活性.对于添加了IN-EPS，LB-EPS和TB-EPS的实验组，厌氧氨氧化颗粒污泥的氨氮去除速率分别增加了6.68%，10.5%和19.29%，厌氧氨氧化菌的生长速率分别增加了18.25%，21%，16.3%.三维荧光光谱法分析发现3种EPS的组成基本相同，以芳香族蛋白质和可溶性微生物产物为主.对EPS中的高丝氨酸内酯类（AHLs）信号分子进行检测，发现EPS中含有3种信号分子，分别是C4-HSL，C6-HSL和C10-HSL，外源投加这3种AHLs到厌氧氨氧化颗粒污泥中，结果发现C4-HSL和C6-HSL可以提高厌氧氨氧化颗粒污泥的活性和生长速率，这是EPS可以提高厌氧氨氧化菌活性的原因.

Abstract

In order to investigate the effect of extracellular polymers (EPS) on the activity of anaerobic ammonium oxidation (ANAMMOX) granular sludge, a certain concentration (30mg/L) of integral extracellular polymers (IN-EPS), loosely bound extracellular polymers (LB-EPS) and tightly bound extracellular polymers (TB-EPS) were added to ANAMMOX granular sludge, respectively. The results indicated that all of the aforementioned EPS could improve the activity of ANAMMOX granular sludge. With regard to the test group including IN-EPS, LB-EPS or TB-EPS, the ammonia nitrogen removal rate of ANAMMOX granular sludge had an increase of 6.68%, 10.5% and 19.29% respectively, while the growth rate of ANAMMOX bacteria increased by 18.25%, 21% and 16.3% respectively. The compositions of three EPSs were basically identical, which is mainly consisted of aromatic proteins and soluble microbial products through three-dimensional fluorescence spectroscopy. And three kinds of acylated homoserine lactones (AHLs) signal molecules in EPSs:C4-HSL, C6-HSL and C10-HSL were found. Additionally, we discovered that C4-HSL and C6-HSL could improve the activity and growth rate of ANAMMOX particles through adding the above EPSs to ANAMMOX granular sludge. This interpreted why EPS could improve the activity of ANAMMOX granular sludge.

导出引用

张亚超, 张晶, 侯爱月, 周荣煊, 梁东博, 张凯, 刘阳, 李军. 胞外聚合物和信号分子对厌氧氨氧化污泥活性的影响[J]. 中国环境科学. 2019, 39(10): 4133-4140

ZHANG Ya-chao, ZHANG Jing, HOU Ai-yue, ZHOU Rong-xuan, LIANG Dong-bo, ZHANG Kai, LIU Yang, LI Jun. Effect of extracellular polymers and signal molecules on the activity of ANAMMOX sludge[J]. China Environmental Science. 2019, 39(10): 4133-4140

中图分类号： X703

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