Effects of anode position on the performance and microbial community of the novel ABR-BEF system
SU Cheng-yuan1, DENG Qiu-jin1, LU Yu-xiang1, QIN Rong-hua1, RUAN Zi-xin1, WEI Jing-wei1, ZHENG Cheng-zhi2
1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, School of Environment and Resources, Guangxi Normal University, Guilin 541004, China;
2. Guangdong Yuegang Water Supply Co., Ltd., Shenzhen 518021, China
In this study, the effect of the position of anode on the efficiency and electricity generation of the novel anaerobic baffle reactor-bioelectricity Fenton (ABR-BEF) system were investigated for treatment traditional Chinese medicine (TCM) wastewater. The components of loosely-bound extracellular polymeric substances (LB-EPS) and tightly-bound extracellular polymeric substances (TB-EPS) in the sludge were also measured. Finally, the high-throughput sequencing technology was used to analyze the variation of microbial community in the system at different anode positions. The results showed that when the position of anode electrode was changed from the 4th compartment to the 3rd and 2nd compartment, the COD removal rate was reduced from 90% to 70% and 65%, respectively, due to the distance between anode and cathode gradually increasing. Meanwhile, the voltage output was declined from 149.8mV to 95.3mV and 50.0mV, and the maximum power density was decreased to 76.78mW/m3 to 55.57mW/m3 and 52.87mW/m3, respectively. However, the removal rate of catechol in the cathode chamber was remained around 95%. After changing the position of anode, the protein content in the LB-EPS and TB-EPS was decreased, especially TB-EPS. In addition, the polysaccharides content of LB-EPS and TB-EPS was the highest when the position of anode was changed into the 3rd compartment. When the position of anode was changed from the 4th compartment to the 3rd and 2nd compartment, the abundance of Methanothrix was increased to 71.09%, 72.47%, 58.03% and 76.79% in each compartment at the genus level of archaea. While the abundance of Deltaproteobacteria, which was a class contained electrogenic bacteria, was reduced to 2.54%, 6.06%, 4.40% and 4.87%, respectively. The increasing of the distance between anode and cathode was leaded to reduce the abundance of electrogenic bacteria, which was an important reason for the decrease of the electricity generation capacity of the ABR-BEF system.
宿程远, 邓秋金, 卢宇翔, 覃容华, 阮子欣, 韦敬威, 郑成志. 阳极位置对新型ABR-BEF系统处理性能及微生物群落的影响[J]. 中国环境科学, 2019, 39(9): 3770-3779.
SU Cheng-yuan, DENG Qiu-jin, LU Yu-xiang, QIN Rong-hua, RUAN Zi-xin, WEI Jing-wei, ZHENG Cheng-zhi. Effects of anode position on the performance and microbial community of the novel ABR-BEF system. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(9): 3770-3779.
李玥,胡奇,高大文.温度对一体式厌氧流化床膜生物反应器运行效能及微生物群落结构的影响[J]. 环境科学, 2018,39(4):1731-1738. Li Y, Hu Q, Gao D W, Effect of temperature on operation efficiency and microbial community structure of integrated anaerobic fluidized bed membrane bioreactor[J]. Environmental Science, 2018,39(4):1731-1738.
[2]
Gulhane M, Pandit P, Khardenavis A, et al. Study of microbial community plasticity for anaerobic digestion of vegetable waste in Anaerobic Baffled Reactor[J]. Renewable Energy, 2017,101:59-66.
[3]
Feng C H, Li F B, Hong J M, et al. Bio-Electro-Fenton process driven by microbial fuel cell for wastewater treatment[J]. Environmental Science and Technology, 2010,44:1875-1880.
[4]
王跃强,胡勇杰,李进,等.新型生物电Fenton法降解偶氮染料:铁复合碳毡阴极研究[J]. 生态环境学报, 2013,22(5):826-831. Wang Y Q, Hu Y J, Li J, et al. New bioelectric Fenton method for degradation of azo dyes:effect of the cathode of iron composite carbon blanket[J]. Journal of Ecological Environment, 2013,22(5):826-831.
[5]
Wang, D L, Hou H J, Hu J P, et al. A bio-electro-Fenton system with a facile anti-biofouling air cathode for efficient degradation of landfill leachate[J]. Chemosphere, 2019,215:173-181.
[6]
曾亚琼,王斌伟,李杰,等.生物电Fenton氧化法去除水体中雌二醇的研究[J]. 北京大学学报, 2016,52(5):939-946. Zeng Y Q, Wang B W, Li Y, et al. Removal of 17β-estradiol in a Bio-electro-Fenton System[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2016,52(5):939-946.
[7]
Sangeetha T, Guo Z C, Liu W Z, et al. Energy recovery evaluation in an up flow microbial electrolysis coupled anaerobic digestion (ME-AD) reactor:Role of electrode positions and hydraulic retention times[J]. Applied Energy, 2017,206:1214-1224.
[8]
余登喜,丁杰,刘先树,等.强化混凝预处理削减中药废水的毒性[J]. 环境工程学报, 2016,10(11):6133-6138. Yu D X, Ding J, Liu X S, et al. Toxicity reduction on pretreatment of traditional Chinese medicine wastewater by enhanced coagulation[J]. Chinese Journal of Environmental Engineering, 2016,10(11):6133-6138.
[9]
Gogoi A, Navgire M, Sarma K C, et al. Fe3O4-CeO2 metal oxide nanocomposite as a Fenton-like heterogeneous catalyst for degradation of catechol[J]. Chemical Engineering Journal, 2017,311:153-162.
[10]
罗勇,张仁铎,李婕,等.以吲哚为燃料的微生物燃料电池降解和产电特性[J]. 中国环境科学, 2010,30(6):770-774. Luo Y, Zhang R D, Li J, et al. Degradation and electrical generation characteristics of microbial fuel cells fueled by indoles[J]. China Environmental Science, 2010,30(6):770-774.
[11]
Jian S, Liang G, Li Q, et al. Structural and functional properties of organic matters in extracellular polymeric substances (EPS) and dissolved organic matters (DOM) after heat pretreatment with waste sludge[J]. Bioresource Technology, 2016,219:614-623.
[12]
Li K, Wei D, Yan T, et al. Responses of soluble microbial products and extracellular polymeric substances to the presence of toxic 2,6-dichlorophenol in aerobic granular sludge system[J]. Journal of Environmental Management, 2016,183:594-600.
[13]
Zhang D, Trzcinski A P, Kunacheva C, et al. Characterization of soluble microbial products (SMPs) in a membrane bioreactor (MBR) treating synthetic wastewater containing pharmaceutical compounds[J]. Water Research, 2016,102:594-606.
[14]
Zhang W, Cao B, Wang D, et al. Influence of wastewater sludge treatment using combined peroxyacetic acid oxidation and inorganic coagulants re-flocculation on characteristics of extracellular polymeric substances (EPS)[J]. Water Research, 2016,88:728-739.
[15]
Miao L, Wang C, Hou J, et al. Response of wastewater biofilm to CuO nanoparticle exposure in terms of extracellular polymeric substances and microbial community structure[J]. Science of the Total Environment, 2017,579:588-597.
[16]
黄薇,刘兰英,吴妙鸿,等.养殖废水处理系统中微生物菌群结构及动态变化[J]. 中国环境科学, 2019,39(2):839-848. Huang W, Liu L Y, Wu M H, et al. Microbial community structure and dynamics in swine wastewater treatment system[J]. China Environmental Science, 2019,39(2):839-848.
[17]
Su C Y, Li W G, Chen M L, et al. Effect of iron-manganese-sepiolite as heterogeneous Fenton-like catalyst on the performance and microbial community of anaerobic granular sludge treatment system[J]. Bioresource Technology, 2016,200:1065-1072.
[18]
Bingrui M, Shanshan L, Sen W, et al. Effect of Fe3O4nanoparticles on composition and spectroscopic characteristics of extracellular polymeric substances from activated sludge[J]. Process Biochemistry, 2018,75:212-220.
[19]
Zhang L W, Dong D M, Hua X Y, et al. Inhibitory effects of extracellular polymeric substances on ofloxacin sorption by natural biofilms[J]. Science of The Total Environment, 2018,625:178-184.
[20]
宋成康,王亚宜,韩海成,等.温度降低对厌氧氨氧化脱氮效能及污泥胞外聚合物的影响[J]. 中国环境科学, 2016,36(7):2006-2013. Song C K, Wang Y Y, Han H C, et al. Effect of temperature reduction on anaerobic ammox denitrification efficiency and extracellular polymer of sludge[J]. China Environmental Science, 2016,36(7):2006-2013.
[21]
Basuvaraj M, Fein J, Liss S N. Protein and polysaccharide content of tightly and loosely bound extracellular polymeric substances and the development of a granular activated sludge floc[J]. Water Research, 2015,82:104-117.
[22]
杨波,徐辉,冯修平,等.SCAR处理城市生活污水的效能及其微生物群落动态分析[J]. 环境科学, 2017,38(5):2021-2029. Yang B, Xu H, Feng X P, et al. Efficacy and dynamic analysis of microbial community in SCAR treatment of municipal sewage[J]. Environmental Science, 2017,38(5):2021-2029.
[23]
Ma J, Quan X, Si X, et al. Responses of anaerobic granule and flocculent sludge to ceria nanoparticles and toxic mechanisms[J]. Bioresource Technology, 2013,149:346-352.
[24]
Lu X Q, Zhen G Y, Estrada A L, et al. Operation performance and granule characterization of upflow anaerobic sludge blanket (UASB) reactor treating wastewater with starch as the sole carbon source[J]. Bioresource Technology, 2015,180:264-273.
[25]
宿程远,郑鹏,卢宇翔,等.磁性纳米铁对厌氧颗粒污泥特性及其微生物群落的影响[J]. 环境科学, 2018,39(3):1316-1324. Su C Y, Zheng P, Lu Y X, et al. Effect of magnetic nanometer iron on the characteristics of anaerobic granular sludge and its microbial community[J]. Environmental Science, 2008,39(3):1316-1324.
[26]
Ronald H O, Alex D G, Ursulo Y P, et al. Bioelectrogenesis with microbial fuel cells (MFCs) using the microalga Chlorella vulgaris and bacterial communities[J]. Electronic Journal of Biotechnology, 2018, 31:34-43.
[27]
Renata T M, Karolina S, Monika K. Bioelectricity production from wood hydrothermal-treatment wastewater:Enhanced power generation in MFC-fed mixed wastewaters[J]. Science of The Total Environment, 2018,634:586-594.
[28]
Lee B, Park J G, Shin W B, et al. Microbial communities change in an anaerobic digestion after application of microbial electrolysis cells[J]. Bioresource Technology, 2017,234:273-280.
