The regulated characteristics and mechanism of tellurite bioreduction by reduced glutathione (GSH) with Shewanella oneidensis MR-1.
ZHANG Wei-hong1, SONG Yuan-yuan1, ZHANG Yan2, ZHAO Rui1, HE Yue1, GUO Jian-bo1
1. Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China;
2. School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China
The regulated characteristics and mechanism of tellurite bioreduction by bioactive small molecule glutathione (GSH) with Shewanella oneidensis MR-1 were investigated in this study. When 0.1, 0.4 and 1.0mmol/L GSH were added to the bioreduction system, the bioreduction efficiency of the tellurite was increased by 55%, 71% and 78%, respectively, compared with the control. Moreover, the concentration of GSH (0.1~1.0mmol/L) was positively correlated with the bioreduction efficiency of tellurite. The culture conditions were optimized by single factor experiment. Under the conditions of 35℃, pH 8.0, 0.4mmol/L GSH, the bioreduction efficiency of tellurite reached 97% within 24h. In addition, six different respiratory inhibitors were used to investigate the regulated electron transfer pathway of tellurite bioreduction by GSH. It was preliminarily determined that the accelerating sites of GSH in the electron transfer chain were NADH reductase, methylnaphthoquinone and FAD dehydrogenase.
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