1. Department of Material Science and Engineering, Henan Institute of Technology, Xinxiang 453003, China; 2. Key Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Wuhan 430072, China
Abstract:In order to efficiently use wasted cyanobacteria and simultaneously yield vigorous phototrophic bacteria, the growth potential and transformation of carbon, nitrogen and phosphorus of Rhodopseudomonas palustris in cyanobacterial substrates were determined. A cyanobacterial concentration greater than 0.87g/L can stimulate the proliferation of R. palustris PUF1under the condition of 30℃, 2000lx. Though less of OD650 in cyanobacterial substrates (3g/L) than that of the ATYP medium at 108h, the concentration of bacteriochlorophyll a (Bchl a) was in similarity showing the more active synthesis of photopigments in cyanobacterial substrates. The addition of smaller molecular organic acids significantly regrew up the PUF1cells in late log phase. Specifically, at 252h and 444h, OD650 in cyanobacterial substrates was enhanced by 191.75% and 269.24%, while Bchl a was 206.68% and 276.17%, respectively. At 444h, OD650 and Bchl a in cyanobacterial substrates were separately 130.88% and 160.62% of the ATYP medium, indicating the superiority of natural cyanobacterial substrate to the ATYP medium for grown PUF1s, especially under a long incubation period. Evaluating the dried biomaterials and dissolved nutrients in cyanobacterial substrates, it indicated the unbalanced distribution of bioavailable carbon, nitrogen and phosphorus, namely surplus nitrogen, lacked carbon and limited phosphorus. It matters of improved bioavailable carbon in cyanobacterial biomasses for the enhanced growth of PUF1and nutrient sequestration.
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