1. College of Forestry, Northwest A&F University, Yangling 712100, China; 2. Institute of Soil and Water Conservation, Ministry of Water Resources, Chinese Academy of Sciences, Yangling 712100, China; 3. University of Chinese Academy of Sciences, Beijing 100190, China
Abstract：Soil microbial activity and community functional diversity at the sedimentary area, erosion area and control area on the slopes with two organic carbon levels were determined by the Biolog-Eco method in the Loess Plateau region so as to explore the effects of erosion and organic carbon levels on the functional diversity of microbial communities. The results showed: 1) The microbial activity and carbon-use ability in the three areas of the slopes under the high carbon level were significantly higher than that under the low carbon level. 2) The microbial activity in the sedimentary area was higher than erosion area under the same carbon levels. However, the carbon-use capacity of microbes in the sedimentary aera was significantly higher than that in the erosion area (P<0.05)at low carbon level. No significant difference was found in the carbon-use capacity of microbes between sedimentary and erosion areas at high carbon level. 3) The microbes had the highest utilization on amino acids and esters but lowest utilization on alcohol and amines among the six types of carbon sources. The principal components 1and 2 extracted from 31 carbon sources could explain 53.8% and 11.9% of variance variables, respectively. Amino acids and ester carbon sources were the main carbon sources that differentiate the PC1, and carbohydrates and acids were the main carbon sources that differentiate the PC2. 4) The Shannon index, richness index and McIntosh index of microbial communities under high carbon levels were significantly greater than that under low carbon levels at the same area on the slopes (P<0.05), However, the diversity of microbial communities in different areas of the slopes was no obvious difference under the same carbon level. The impact of erosion on the functional diversity of microbial communities was mainly caused by the difference of microbial activity, which related to the organic carbon level. The carbon-use ability of microbes in different areas was significantly different under a low organic carbon level, but no obvious difference was found under the higher organic carbon level.
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