The inhibiting effect on inhibition of chlorella growth by liverwort aqueous extract
LU Shi-huan1, CHEN Bin1, YIN Lu1, HAO Xiao-jing1, DING Jia-feng1, WANG Chang-zhi2, WANG Xiao-min2, MEI Rong-wu2, WU Yu-huan1, ZHANG Hang-jun1
1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310018, china;
2. Environmental Science Research and Design Institute of Zhejiang Province, Hangzhou 310012, china
The suppressive effect of liverwort aqueous extract on chlorella growth was studied and a cheap, efficient and low-toxic algicide was selected. Thus, the difference in suppression effect of liverwort and extensive straw on chlorella growth was also compared and analyzed. The inhibition of chlorella growth reached 88.17% after culturing 120h in 0.5g/L liverwort aqueous extract, while, the inhibition of the straw aqueous extract only reached 66.28%. The inhibition of liverwort aqueous extract showed better efficacy than straw aqueous extract at the same conditions. The high concentration of liverwort aqueous extract (25g/L) could reduce the photosynthetic activity of chlorella, while low concentration of liverwort aqueous extract (1g/L) revealed insignificant effects on the photosynthetic activity of chlorella. Compared the suppressive effect of ethanol and deionized water extracts on chlorella, the organic allelopathic components of liverwort aqueous extract inhibited the growth of chlorella. Moreover, the algal inhibition effect of liverwort aqueous extract was similar to three single flavonoids, which were myricetin, parsley and lutein.
Paerl H W, Otten T G. Blooms Bite the Hand That Feeds Them[J]. Science, 2013,342(6157):433-434.
[2]
Bollapragada S, Sodhi M M S. Ultrasonic treatment for microbiological control of water systems[J]. Ultrasonics Sonochemistry, 2010,17(6):1041-1048.
[3]
Chen L, Mao F, Kirumba G C, et al. Changes in metabolites, antioxidant system, and gene expression in Microcystis aeruginosa under sodium chloride stress[J]. Ecotoxicology & Environmental Safety, 2015,122:126-135.
[4]
Jancula D, Blahoslav Maršálek. Critical review of actually available chemical compounds for prevention and management of cyanobacterial blooms[J]. Chemosphere, 2011,85(9):1415-1422.
[5]
陈超,范帆,史小丽,等.化学氧化剂对水华蓝藻的控制研究[J].中国环境科学, 2018,38(11):4307-4313. Chen C, Fan F, Shi X L, et al. Research on the Control of Chemical Oxidants over Water-Blooming Cyanobacteria[J]. China Environmental Science, 2018,38(11):4307-4313
[6]
RICE E L. Allelopathy[M]. The second edition. London:Academic Press, 1984:1-2.
[7]
Willis R J. The historical bases of the concept of allelopathy[J]. Journal of the History of Biology, 1985,18(1):71-102.
[8]
张庭廷,吴安平,何梅,等.酚酸类物质对水华藻类的化感作用及其机理[J]. 中国环境科学, 2007,27(4):472-476. Zhang T T, Wu A P, He M, et al. The allelopathy and its mechanism of phenolic acids on water-bloom algae[J]. China Environmental Science, 2007,27(4):472-476
[9]
谢树莲,王捷,刘琪,等.植物化感作用控藻研究进展[J]. 山西大学学报(自然科学版), 2017,40(3):652-660. Xie S L, Wang J, Liu Q, et al. Research progress on plant allelopathic effects for algal control[J]. Journal of shanxi university, 2017,40(3):652-660.
[10]
胡光济,张维昊,尚吟竹,等.菖蒲干体提取液对两种水华藻类生长的影响[J]. 应用生态学报, 2009,20(9):2277-2282. Hu G J, Zhang W H, Shang Y Z, et al. Inhibitory effects of dry acours calamus extracts on the growth of two water bloom-forming algal species[J]. Chinese Journal of Applied Ecology, 2009,20(9):2277-2282.
[11]
陈卫民,张清敏,戴树桂.苦草与铜绿微囊藻的相互化感作用[J]. 中国环境科学, 2009,29(2):147-151. Chen W M, Zhang Q M, Dai S G. The mutual allelopathy of Vallisneria spiralis Linn. and Microcystis aeruginosa.[J]. China Environmental Science, 2018,38(11):4307-4313.
[12]
张之浩,李威,吴晓芙.6种沉水植物对富营养化水体化感抑藻效果研究[J]. 湘潭大学自然科学学报, 2017,39(2):55-60. Zhang Z H, Li W, Wu X F. Allelopathic effects of six submerged macrophytes on alage growth in eutrophied water[J]. Natural Science Journal of Xiangtan University, 2017,39(2):55-60.
[13]
沈应时,张云霄,张翠英,等.5种植物沉床系统对富营养化水体修复效果研究[J]. 环境保护科学, 2017,43(1):71-76. Shen Y S, Zhang Y X, Zhang C Y, et al. Experimental study of the effects of 5species of plant-mattress system on eutrophic water remediation[J]. Environmental Protection Science, 2017,43(1):71-76.
[14]
沙伟,韩继臣.7种苔藓植物水提液对玉米种子萌发及幼苗生长的影响[J]. 种子, 2008,27(11):37-39. Sha W, Han J C. Effects of extracting liquor from seven species of bryophytes on seed germination and seedling growth of maize[J]. Seed, 2008,27(11):37-39.
[15]
Zhang C, Yi Y L, Hao K, et al. Algicidal activity of Salvia miltiorrhiza bung on Microcystis aeruginosa towards identification of algicidal substance and determination of inhibition mechanism[J]. Chemosphere, 2013,93(6):997-1004.
[16]
Campbell D, Hurry V, Clarke A K, et al. Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation.[J]. Microbiology and molecular biology reviews:MMBR, 1998,62(3):667-683.
[17]
Henriques F S. Leaf chlorophyll fluorescence:Background and fundamentals for plant biologists[J]. Botanical Review, 2009, 75(3):249-270.
[18]
Victoria López-Rodas, Fernando Marvá, Costas E, et al. Microalgal adaptation to a stressful environment (acidic, metal-rich mine waters) could be due to selection of pre-selective mutants originating in non-extreme environments[J]. Environmental and Experimental Botany, 2008,64(1):43-48.
[19]
Wang X, Jiang C, Szeto Y T, et al. Effects of dracontomelon duperreanum efoliation extract on Microcystis aeruginosa:physiological and morphological aspects[J]. Environmental Science and Pollution Research, 2016,23(9):8731-8740.
[20]
Welch I M, Barrett P R F, Gibson M T, et al. Barley straw as an inhibitor of algal growth I:studies in the Chesterfield Canal[J]. Journal of Applied Phycology, 1990,2(3):231-239.
[21]
Chen W, Westerhoff P, Leenheer J A, et al. Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter[J]. Environmental Science & Technology, 2003, 37(24):5701-5710.
[22]
Gross, Elisabeth M. Allelopathy of Aquatic Autotrophs[J]. Critical Reviews in Plant Sciences, 2003,22(3):313-339.
[23]
汪庆,罗宣.苔藓植物的主要次生代谢产物与有害生物防治[J]. 贵州科学, 2001,19(4):93-100. Wang Q, Luo X. The application of bryophytes in plant prevention[J]. Guizhou Science, 2001,19(4):93-100.
[24]
Everall N C, Lees D R. The use of barley-straw to control general and blue-green algal growth in a Derbyshire reservoir[J]. Water Research, 1996,30(2):0-276.
[25]
Richardson R B P M. The chemistry of allelopathy:biochemical interactions among plants[J]. Brittonia, 1985,37(3):251.
[26]
张彬,郭劲松,方芳,等.植物化感抑藻的作用机理[J]. 生态学杂志, 2010,29(9):1846-1851. Zhang B, Guo J S, Fang F, et al. Action mechanisms of allelopathy in inhibiting algae[J]. Chinese Journal of Ecology, 2010,29(9):1846-1851.
[27]
Wang X, Szeto Y T, Jiang C, et al. Effects of dracontomelon duperreanum leaf litter on the growth and photosynthesis of microcystis aeruginosa[J]. Bulletin of Environmental Contamination and Toxicology, 2018,100(5):690-694.
[28]
Hua Q, Liu Y G,Yan Z L, et al. Allelopathic effect of the rice straw aqueous extract on the growth of Microcystis aeruginosa[J]. Ecotoxicology and Environmental Safety, 2018,148:953-959.
[29]
Asakawa Y. Biologically active compounds from bryophytes[J]. Journal of the Hattori Botanical Laboratory, 2007,79(4):557-580.
