The growth and lipid productivity of original Chlorella pyrenoidosa and its ion implantation mutant strains were studied. The results showed that the lipid productivity of the mutant strain was 32.5mg/(L·d), 23.81% higher than that of the original strain. According to the fatty acid methyl ester analysis by gas chromatography, ion implantation did not change the composition of fatty acid, but enhanced the content of monounsaturated fatty acids, which was conducive to the enhancement of biodiesel quality. After cultivation, the effluent quality was also found to reach the emission standard of first level A, and the mutant showed better degradation ability than the original strain. Phylogenetic tree showed that the mutant and the original strains were belonged to interspecific variation.
涂仁杰, 金文标, 韩松芳, 周旭, 陈洪一. 离子注入突变藻株在城市污水中的产油特性[J]. 中国环境科学, 2017, 37(10): 3735-3740.
TU Ren-jie, JIN Wen-biao, HAN Song-fang, ZHOU Xu, CHEN Hong-yi. The characteristics of lipid production of Chlorella pyrenoidosa mutant by ion implantation through municipal wastewater cultivation. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(10): 3735-3740.
Chisti Y. Constraints to commercialization of algal fuels[J]. Journal of Biotechnology, 2013,167(3):201-214.
[2]
Han S F, Jin W B, Tu R J, et al. Biofuel production from microalgae as feedstock:current status and potential[J]. Critical Reviews in Biotechnology, 2015,35(2):255-268.
[3]
Abomohra A E, Jin W B, Tu R J, et al. Microalgal biomass production as a sustainable feedstock for biodiesel:current status andperspectives[J]. Renewable & Sustainable Energy Reviews, 2016,64(64):596-606.
[4]
Yang J, Xu M, Zhang M X, et al. Life-cycle analysis on biodiesel production from microalgae:water footprint and nutrients balance[J]. Bioresource Technology, 2011,102:159-165.
[5]
Morales-Amaral M d M, Gómez-Serrano C, Acién F G, et al. Production of microalgae using centrate from anaerobic digestion as the nutrient source[J]. Algal Research, 2015,9:297-305.
Ramsundar P, Guldhe A, Singh P, et al. Assessment of municipal wastewaters at various stages of treatment process as potential growth media for Chlorella sorokiniana under different modes of cultivation[J]. Bioresource Technology, 2017,227:82-92.
[8]
Mahapatra D M, Chanakya H N, Ramachandra T V. Bioremediation and lipid synthesis through mixotrophic algal consortia in municipal wastewater[J]. Bioresource Technology, 2014,168:142-150.
[9]
Ryu B G, Kim E J, Kim H S, et al. Simultaneous treatment of municipal wastewater and biodiesel production by cultivation of Chlorella vulgaris with indigenous wastewater bacteria[J]. Biotechnology and Bioprocess Engineering, 2014,19(2):201-210.
[10]
Muñoz R, Guieysse B. Algal-bacterial processes for the treatment of hazardous contaminants:a review[J]. Water Research, 2006, 40:2799-2815.
[11]
Abdelaziz A E M, Leite G B, Belhaj M A, et al. Screening microalgae native to Quebec for wastewater treatment and biodiesel production[J]. Bioresource Technology, 2014,157:140-148.
[12]
Massimi R, Kirkwood A E. Screening microalgae isolated from urban storm and wastewater systems as feedstock for biofuel[J]. PeerJ, 2016,4:e2396.
[13]
Tu R J, Jin W B, Wang M, et al. Improving of lipid productivity of the biodiesel promising green microalga Chlorella pyrenoidosa via low-energy ion implantation[J]. Journal of Applied Phycology, 2016,28(4):2159-2166.
[14]
Stainier R Y, Kunisawa R, Mandel M, et al. Purification and properties of unicellular blue-green algae (order Chroococcales)[J]. Bacteriology Reviews, 1971,35(2):171-205.
[15]
Folch J, Lees M, Stanley G H S. A simple method for the isolation and purification of total lipids from animal tissues[J]. Journal of Biological Chemistry, 1957,226:497-509.
[16]
Kaczmarzyk D, Fulda M. Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling[J]. Plant Physiology, 2010,152(3):1598-1610.
[17]
国家环保总局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002.
[18]
GB 11914-89水质化学需氧量的测定重铬酸盐法[S].
[19]
GB 7479-87水质铵的测定纳氏试剂比色法[S].
[20]
GB 11894-89水质总氮的测定碱性过硫酸钾消解紫外分光光度法[S].
[21]
GB 11893-89水质总磷的测定钼酸铵分光光度法[S].
[22]
Sharma K K, Schuhmann H, Schenk P M. High lipid induction in microalgae for biodiesel production[J]. Energies, 2012,5(5):1532-1553.
Vidyashankar S, VenuGopal K S, Swarnalatha G V, et al. Characterization of fatty acids and hydrocarbons of chlorophycean microalgae towards their use as biofuel source[J]. Biomass & Bioenergy, 2015,77:75-91.
[25]
Song M, Pei H, Hu W, et al. Evaluation of the potential of 10microalgal strains for biodiesel production[J]. Bioresource Technology, 2013,141:245-251.
[26]
Ma Y, Wang Z, Yu C, et al. Evaluation of the potential of 9Nannochloropsis strains for biodiesel production[J]. Bioresource Technology, 2014,167:503-509.
[27]
Stansell G R, Gray V M, Sym S D. Microalgal fatty acid composition:implications for biodiesel quality[J]. Journal of Applied Phycology, 2012,24(4):791-801.
Hoekman S K, Broch A, Robbins C, et al. Review of biodiesel composition, properties, and specifications[J]. Renewable & Sustainable Energy Reviews, 2012,16:143-169.
