Remediation effects of the different biochars on crude-oil contaminated soil in eastern Gansu Province of the Loess Plateau
WANG Jin-cheng1,2,3, JING Ming-bo1,2, ZHANG Shao-peng4, LIU Guang-xiu3, CHEN Tuo3, ZHOU Tian-lin1,2, WU Sheng-wei5
1. College of Life Science and Technology, Longdong University, Qingyang 745000, China;
2. University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-Resources in Gansu Province, Qingyang 745000, China;
3. Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Northwest Institute of ECO-Environment and Resource, Chinese Academy of Sciences, Lanzhou 730000, China;
4. The 11 th Oil Production Plant of PCOC, Qingyang 745000, China;
5. Xi'an H & F Environment Protection Engineering Company Limited, Xi'an 710068, China
In order to analyze and compare the remediation effects of the different biochars on crude-oil contaminated soil in eastern Gansu Province of the Loess Plateau, three types of biochars were fabricated from maize straw (JYB), wheat straw (JXB), and apple tree branch (JYB), together with the indigenous plant, Calendula officinalis, were taken as tested plant (J) to do the in situ ecological restoration for four months in the second oil production plant of the Changqing Oil Field Company. The changes of environmental factors, including soil total petroleum hydrocarbons (TPH) degradation rate, plant growth indices, soil physicochemical traits, enzyme activity, and soil microbial community structure were investigated by the conventional methods. The soil TPH degradation rate in the JYB[(59.89±2.23)%] and JGB[(58.17±1.89)%] treatments was relatively higher than that in the JXB and J treatments (P < 0.05). The plant height, plant aboveground and underground dry weight of Calendula officinalis, as well as its rhizospheric soil available N, available P, available K, and activity of soil dehydrogenase and polyphenol oxidase in the JYB, JXB, and JGB treatments were significantly higher than that in other treatments. While compared with JXB treatment, the plant root length, soil catalase and urease activity in JYB and JGB treatments were significantly increase by 23.81%、18.89%, 153.12%、80.13% and 188.66%、117.45%, respectively(P < 0.05). Furthermore, the dominant genera of soil microbial community with capacity of crude-oil degradation in the JYB and JGB treatments, including Sphingomonas, Oleispira, Simiduia, Salinimicrobium, Acinetobacter, Rhodococcus, Alcanivorax, and Nocardia, were significantly higher than that in the JXB and J treatments (P < 0.05). All of the results mentioned above indicated that the soil microbial community structures in the JYB and JGB treatments were different from that of the JXB and J treatments, which could be affected by relatively higher value of soil polyphenol oxidase activity (R2=0.9786, P=0.001), soil dehydrogenase activity (R2=0.9713, P=0.005), soil available N (R2=0.6112, P=0.046), Shannon index (R2=0.7311, P=0.036) and Chao1index (R2=0.6361, P=0.041) of soil microbial community, might be the reason why the soil TPH degradation rate in the JYB and JGB treatments was relatively higher than that of other treatments. Consequently, we proposed that maize straw biochar and apple tree branch would be preferred as the crude-oil contaminated soil remediation conditioner for phytoremediation in eastern Gansu province of loess plateau.
王金成, 井明博, 张绍鹏, 刘光琇, 陈拓, 周天林, 吴胜伟. 不同生物质炭对陇东黄土高原石油污染土壤的修复作用[J]. 中国环境科学, 2020, 40(6): 2565-2576.
WANG Jin-cheng, JING Ming-bo, ZHANG Shao-peng, LIU Guang-xiu, CHEN Tuo, ZHOU Tian-lin, WU Sheng-wei. Remediation effects of the different biochars on crude-oil contaminated soil in eastern Gansu Province of the Loess Plateau. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(6): 2565-2576.
Barati M, Bakhtiari F, Mowla D, et al.Total petroleum hydrocarbon degradation in contaminated soilas affected by plants growth and biochar [J].Environmental Earth Sciences, 2017,76:688-699.
[2]
王艳杰,李法云,荣湘民,等.生物质材料与营养物配施对石油污染土壤的修复[J].农业环境科学学报, 2018,37(2):232-238. Wang Y J, Li F Y, Rong X M, et al.Remediation of petroleum-contaminated soils by combined application of biomass materials and nutrients [J].Journal of Agro-Environment Science, 2018,37(2): 232-238.
[3]
Sauma L, Jimenez M B, Crowleya D.Influence of biochar and compost on phytoremediation of oil-contaminated soil [J].International Journal of Phytoremediation, 2018,20(1):54-60.
[4]
Cao Y N, Yang B S, Song Z H, et al.Wheat straw biochar amendments on the removal of polycyclicaromatic hydrocarbons (PAHs) in contaminated soil [J].Ecotoxicology and Environmental Safety, 2016, 130:248-255.
[5]
石丽芳,李法云,王艳杰,等.不同生物质炭对辽河油田石油污染土壤总烃及各组分修复效果研究[J].生态环境学报, 2019,28(1):199-206. SHI L F, LI F Y, WANG Y J, et al.Removal of total petroleum hydrocarbons and specific components by various biochars in petroleum contaminated soil from Liaohe oilfield [J].Ecology and Environmental Sciences, 2019,28(1):199-206.
[6]
Zhen M N, Chen H K, Liu Q L, et al.Combination of rhamnolipid and biocharin assisting phytoremediation of petroleumhydrocarbon contaminated soil using Spartinaanglica [J].Journal of Environmental Sciences, 2019,85:107-118.
[7]
Wu M L, Li W, Dick W A, et al.Bioremediation of hydrocarbon degradation in a petroleum-contaminated soil and microbial population and activity determination [J].Chemosphere, 2017,169: 124-130.
[8]
González-Chávez MCA, Carrillo-González R, Godínez M I H, et al.Jatropha curcas and assisted phytoremediationof a mine tailing with biochar and a mycorrhizalfungus [J].International Journal of Phytoremediation, 2017,19(2):174-182.
[9]
Zhang M Y, Wang J, Shahla H B, et al.Assisted phytoremediation of a co-contaminated soil with biochar amendment: Contaminant removals and bacterial community properties [J].Geoderma, 2019,348:115-123.
[10]
Ni N, Wang F, Song Y, et al.Mechanisms of biochar reducing the bioaccumulation of PAHs in rice from soil: Degradation stimulation vs immobilization [J].Chemosphere, 2018,196:288-296.
[11]
王金成,井明博,段春艳,等.陇东黄土高原石油污染土壤环境因子对金盏菊(Calendula officinalis)-微生物联合修复的响应[J].环境科学学报, 2015,35(9):2971-2981. Wang J C, Jing M B, Duan C Y, et al.Environmental factors responses to petroleum-contaminated soil in situ remediation by using a combination of Calendula officinalis and microbial agent in eastern Gansu Province [J].Acta Scientiae Circumstantiae, 2015,35(9):2971-2981.
[12]
朱文英,唐景春.小麦秸秆生物炭对石油烃污染土壤的修复作用[J].农业资源与环境学报, 2014,31(3):259-264. Zhu W Y, Tang J C.Remediation of wheat-straw-biochar on petroleum-polluted soil [J].Journal of Agricultural Resources and Environment, 2014,31(3):259-264.
[13]
祁迎春,王建,同延安,等.陕北石油污染土壤植物修复品种的筛选[J].生态科学, 2015,34(1):148-153. Qi Y C, Wang J, Tong Y A, et al.Screening of weed plants for phytoremediation of petroleum-contaminated soils [J].Ecological Science, 2015,34(1):148-153.
[14]
王金成,周天林,井明博,等.陇东黄土高原地区石油污泥原位修复过程中土壤主要肥力指标动态变化分析[J].环境科学学报, 2015,35(1):280-287. Wang J C, Zhou T L, Jing M B, et al.Fertility dynamics of petroleum-contaminated sludge during in situ remediation in eastern Gansu Province [J].Acta Scientiae Circumstantiae, 2015,35(1):280-287.
[15]
王金成,井明博,段春燕,等.石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响[J].水土保持通报, 2017,37(1):9-16. Wang J C, Jing M B, Duan C Y, et al.Impact of crude oil pollution on soil environmental factors and microbial community structure in eastern Gansu Province [J].Bulletin of Soil and Water Conservation, 2017,37(1):9-16.
[16]
吴蔓莉,祁燕云,祝长成,等.堆肥对土壤中石油烃的去除及微生物群落的影响[J].中国环境科学, 2018,38(8):3042-3048. Wu M L, Qi Y Y, Zhu C C, et al.Influence of compost amendment on hydrocarbon degradation and microbial communities in petroleum contaminated soil [J].China Environmental Science, 2018,38(8): 3042-3048.
[17]
Han T, Zhao Z P, Bartlam M, et al.Combination of biochar amendment and phytoremediation for hydrocarbon removal in petroleum-contaminated soil [J].Environmental Science and Pollution Research, 2016,23(21):21219-21228.
[18]
杨放,李心清,王兵,等.热解材料对生物炭理化性质的影响[J].农业环境科学学报, 2015,34(9):1822-1828. Yang F, Lee X Q, Wang B, et al.Effects of different feedstocks on physicochemical characteristics of pyrolyzed biochars [J].Journal of Agro-Environment Science, 2015,34(9):1822-1828.
[19]
Lei O Y, Zhang R D.Effects of biochars derived from different feedstocks and pyrolysis temperatures on soil physical and hydraulic properties [J].Journal of Soils and Sediments, 2013,13(9):1561-1572.
[20]
孙樱萁,郭建斌,李子富,等.生物质炭基肥料对黑麦草生长特性的影响[J].西北农林科技大学学报(自然科学版), 2016,44(12):117-123. Sun Y Q, Guo J B, Li Z F, et al.Influence of biochar-based fertilizers on growth characteristics of ryegrass [J].Journal of Northwest A&F University (Social Science Edition), 2016,44(12):117-123.
[21]
宋曦,王金成,井明博,等.紫花苜蓿对陇东黄土高原油污土壤场地生态修复的综合响应[J].草业科学, 2019,36(7):1754-1764. Song X, Wang J C, Jing M B, et al.Comprehensive response of Medicago sativa when used to ecologically remediate a site in the eastern region of the Loess Plateau contaminated with different concentrations of oil [J].Pratacultural Science, 2019,36(7):1754-1764.
[22]
Al-Baldawi I A, Abdullah S R S, Anuar N, et al.Phytodegradation of total petroleum hydrocarbon (TPH) in diesel-contaminated water using Scirpus grossus [J].Ecological Engineering, 2015,74:463-473.
[23]
Hussain F, Hussain I, Khan A H A, et al.Combined application of biochar,compost,and bacterial consortia with Italian ryegrass enhanced phytoremediation of petroleum hydrocarbon contaminated soil [J].Environmental and Experimental Botany, 2018,153:80-88.
[24]
周桂玉,窦森,刘世杰.生物质炭结构性质及其对土壤有效养分和腐殖质组成的影响[J].农业环境科学学报, 2011,30(10):2075-2080. Zhou G Y, Dou S, Liu S J.The structural characteristics of biochar and its effects on soil available nutrients and humus composition [J].Journal of Agro-Environment Science, 2011,30(10):2075-2080.
[25]
Wang Y J, Li F Y, Rong X M, et al.Remediation of petroleum-contaminated soil using bulrush straw powder, biochar and nutrients [J].Bulletin of Environmental Contamination and Toxicology, 2017, 98:690-697.
[26]
Chintal A R, Mollinedo L M, Schumacher T E, et al.Effect of biochar on chemical properties of acidic soil [J].Archives of Agronomy and Soil Science, 2014,60(3):393-404.
[27]
Yuan J H, Xu R K, Zhang H.The forms of alkalis in the biochar produced from crop residues at different temperatures [J].Bioresource Technology, 2011,102(3):3488-3497.
[28]
Yasser M A, Evgenia B, Yong S O, et al.Effects of polyacrylamide, biopolymer, and biochar on decomposition of soil organic matter and plant residues as determined by 14C and enzyme activities [J].European Journal of Soil Biology, 2012,48:1-10.
[29]
Lori A P, Charles W G, Richard E F, et al.Plant root exudates impact the hydrocarbon degradation potential of a weathered-hydrocarbon contaminated soil [J].Applied Soil Ecology, 2012,52:56-64.
[30]
Yemisi D O, Olugbenga S T, Oladele J O, et al.Biodegradation of crude petroleum by bacterial consortia from oil-contaminated soils in Ota, Ogun State, South-Western, Nigeria [J], Environmental Technology & Innovation, 2018,12:230-242.
[31]
Ameloot N, Neve S D, Jegajeevagan K, et al.Short-term CO2 and N2O emissions and microbial properties of biochar amended sandy loam soils [J].Soil Biology and Biochemistry, 2013,57:401-410.
[32]
Gong X M, Huang D L, Liu Y G, et al.Biochar facilitated the phytoremediation of cadmium contaminated sediments: Metal behavior, plant toxicity, and microbial activity [J].Science of the Total Environment, 2019,666:1126-1133.
[33]
卜晓莉,薛建辉.生物炭对土壤生境及植物生长影响的研究进展[J].生态环境学报, 2014,23(3):535-540. Bu X L, Xue J H.Biochar effects on soil habitat and plant growth: A review [J].Ecology and Environmental Sciences, 2014,23(3):535-540.
[34]
Qin G, Gong D, Fan M Y.Bioremediation of petroleum-contaminated soil by biostimulation amended with biochar [J].International Biodeterioration & Biodegradation, 2013,85:150-155.
[35]
Onotasamiderhi T I, Paola M, Russell J D, et al.Impacts of activated carbon amendments, added from the start or after five months, on the microbiology and outcomes of crude oil bioremediation in soil [J].International Biodeterioration & Biodegradation, 2019,142:1-10.
[36]
王彤彤,王晓琳,任志胜,等.不同原料制备的生物炭形貌结构及表面特性研究,环境科学与技术, 2017,40(1):42-48. Wang T T, Wang X L, Ren Z S, et al.Microscopic morphology and surface features of biochars derivedfrom different raw materials [J].Environmental Science & Technology, 2017,40(1):42-48.
[37]
Kong L L, Gao Y Y, Zhou Q X, et al.Biochar accelerates PAHs biodegradation in petroleum-polluted soil by biostimulation strategy [J].Journal of Hazardous Materials, 2018,343:276-284.
[38]
Cheng L J, Wang Y N, Cai Z, et al.Phytoremediation of petroleum hydrocarbon contaminated saline-alkali soil by wild ornamental Iridaceae species [J].International Journal of Phytoremediation, 2017, 19(3):300-308.
[39]
王宝山,温成成,孙秦川,等.石油烃类污染对青藏高原北麓河地区冻区土壤微生物多样性的影响[J].环境工程学报, 2018,12(10): 2917-2928. Wang B S, Wen C C, Sun Qi C, et al.Effects of petroleum hydrocarbon pollution on soil microbial diversity in permafrost region of Beiluhe area of Qinghai-Tibet plateau [J].Chinese Journal of Environmental Engineering, 2018,12(10):2917-2928.
[40]
王丽萍,李丹,许锐伟,等.专性菌系对石油烃污染土壤的修复性能[J].中国环境科学, 2018,38(4):1417-1423. Li P, Li D, Xu R W, et al.Remediation performance of constructed specific bacteria on petroleum hydrocarbon contaminated soil [J].China Environmental Science, 2018,38(4):1417-1423.
[41]
田伟君,王勇梅,孙会梅,等.石油输入对河口芦苇湿地根际微生物的影响[J].中国环境科学, 2014,34(10):2676-2683. Tian W J, Wang Y M, Sun H M, et al.Effect of oil pollution on community structure of plants rhizospheric microorganisms in estuarine wetland [J].China Environmental Science, 2014,34(10): 2676-2683.
[42]
Jahantab E, Jafari M, Motesharezadeh B, et al.Remediation of petroleum-contaminated soils using Stipagrostis plumosa, Calotropis procera and Medicago sativa under different organic amendment treatments, Ecopersia, 2018,6(2):101-109.
