Effects of Broussonetia papyrifera on the enzyme activities and microbial community structure of heavy metal-contaminated soil were studied using greenhouse experiment. Results showed that the enzyme activities and microbial diversity were obviously enhanced in contaminated soil with B. papyrifera remediation. Compared with the soil without B. papyrifera, the contents of sucrase and acid phosphatase in soil remediated with B. papyrifera were significantly (P< 0.05) increased by 3.12 and 2.29 times, respectively, after 270 days of cultivation. Meanwhile, the relationship was significantly (P< 0.05) negative between the content of dehydrogenase and available As, Cd, Pb, Zn and Cu, that of sucrase and available Cd, that of acid phosphatase and available Cd and Cu, respectively. According to 16S and 18S rDNA PCR-DGGE analyses, the diversities of bacteria and arbuscular mycorrhizal fungus in contaminated soil were enhanced with the growth of B. papyrifera. The results suggested that the biological quality in heavy metals contaminated soil could be effectively improved with B. papyrifera remediation. However, the content of available heavy metals was scarcely declined in contaminated soil for 270 days of cultivation. Therefore, it is necessary to improve the potential of ecological remediation for metals-contaminated soil by combining B. papyrifera with additional physical and chemical methods.
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