渭河杨凌段水系中头孢曲松耐药菌和耐药基因

刘浩宇; 朱瑜; 王惠芝; 盛焕精; 杨保伟

PDF(2600 KB)

中国环境科学 ›› 2020, Vol. 40 ›› Issue (7) : 3087-3096.

环境生态

渭河杨凌段水系中头孢曲松耐药菌和耐药基因

刘浩宇¹, 朱瑜², 王惠芝¹, 盛焕精¹, 杨保伟¹

作者信息 +

Prevalence of ceftriaxone-resistant bacteria and drug-resistance encoding genes in Weihe River and its tributaries in Yangling Section

LIU Hao-yu¹, ZHU Yu², WANG Hui-zhi¹, SHENG Huan-jing¹, YANG Bao-wei¹

Author information +

文章历史 +

摘要

调研了杨凌抗生素使用和过期抗生素处理情况，研究了渭河杨凌段及其在杨凌境内主要支流中耐药菌（主要为耐头孢曲松细菌）及常见耐药基因的分布特征.杨凌区β-内酰胺类药物占抗生素总用量的55.0%，64.0%的过期抗生素被合理处理；水样中耐头孢曲松细菌平均浓度均大于500CFU/mL，分离到5种气单胞菌（n=44）和1株大肠杆菌；45株（100.0%）菌全部耐受氨苄西林，阿莫西林/克拉维酸和头孢曲松，多重耐药率为95.6%；水样总DNA中ampC，sul1，sul2，sul3，tetB，tetC，tetM，aadA，cmlA，catA，bla_TEM，bla_NDM，aph（2’）-Id和aac（6’）-Ib检出率均为100.0%，15株耐头孢曲松代表菌中ampC与bla_TEM检出率均为100.0%.渭河杨凌段及其主要支流中多重耐药细菌检出率较高，耐药基因多样化.研究可为杨凌区水体中耐药菌污染治理提供参考.

Abstract

In this study, the situation of antibiotic usage and disposal of expired antibiotic in Yangling city, and the distribution of antibiotic-resistant bacteria (mainly ceftriaxone resistant bacteria) and antibiotic resistance genes in Weihe River and its main tributaries in Yangling city were studied. The results indicate that β-lactam accounted for 55.0% of total antibiotics usage in Yangling, and 64.0% of the expired antibiotics were properly disposed. The numbers of ceftriaxone-resistant bacteria in all water samples were higher than 500CFU/mL. Five Aeromonas species (44isolates) and one Escherichia coli isolate were recovered. All (100.0%) isolates were resistant to ampicillin, amoxicillin/clavulanate and ceftriaxone, and the rate of multidrug resistant isolates was 95.6%. The detection rates of ampC, sul1, sul2, sul3, tetB, tetC, tetM, aadA, cmlA, catA, bla_TEM, bla_NDM, aph(2')-Id and aac(6')-Ib in 15water samples were 100.0%, and ampC and bla_TEM were detected in all 15ceftriaxone resistant isolates. Frequent occurrence of multidrug resistant bacteria and drug resistance genes were demonstrated in Weihe River and its tributaries in Yangling Section. This study can provide useful data for reduction of drug resistant bacteria and gene in the water in Yangling, Shaanxi.

导出引用

刘浩宇, 朱瑜, 王惠芝, 盛焕精, 杨保伟. 渭河杨凌段水系中头孢曲松耐药菌和耐药基因[J]. 中国环境科学. 2020, 40(7): 3087-3096

LIU Hao-yu, ZHU Yu, WANG Hui-zhi, SHENG Huan-jing, YANG Bao-wei. Prevalence of ceftriaxone-resistant bacteria and drug-resistance encoding genes in Weihe River and its tributaries in Yangling Section[J]. China Environmental Science. 2020, 40(7): 3087-3096

中图分类号： X523

参考文献

[1] Sarmah A K, Meyer M T, Boxall A B A. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment[J]. Chemosphere, 2006,65(5):725-759.
[2] Zhou L J, Ying G G, Liu S, et al. Excretion masses and environmental occurrence of antibiotics in typical swine and dairy cattle farms in China. Sci[J]. Total Environ, 2013,444:183-195.
[3] Baym M, Lieberman T D, Kelsic E D, et al. Spatiotemporal microbial evolution on antibiotic landscapes[J]. Science, 2016,353(6304):1147-1151.
[4] Global action plan on antimicrobial resistance[R]. WHO, 2015.
[5] Hao R, Zhao R, Qiu S, et al. Antibiotics crisis in China[J]. Science, 2015,348(6239):1100-1101.
[6] Zhang Q Q, Ying G G, Pan C G, et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of China:source analysis, multimedia modeling, and linkage to bacterial resistance[J]. Environmental science & technology, 2015,49(11):6772-6782.
[7] Fresia P, Antelo V, Salazar C, et al. Urban metagenomics uncover antibiotic resistance reservoirs in coastal beach and sewage waters[J]. Microbiome, 2019,7(1):35.
[8] Xu W H, Zhang G, Zou S C, et al. A preliminary investigation on the occurrence and distribution of antibiotics in the Yellow River and its tributaries, China.[J]. Water environment research:a research publication of the Water Environment Federation, 2009,81(3):248-54.
[9] 潘文进,鄱阳湖流域抗生素耐药基因分布特征及与新型粪便污染指示菌的关系[D]. 南昌:南昌大学, 2018. Pan W J. Occurrence of antibiotice resistance genes and their relationship with new fecal indicator bacteria in Poyang lake basin[D]. Nanchang:Nanchang University, 2018.
[10] Hsu J T, Chen C Y, Young C W, et al. Prevalence of sulfonamide-resistant bacteria, resistance genes and integron-associated horizontal gene transfer in natural water bodies and soils adjacent to a swine feedlot in northern Taiwan[J]. Journal of Hazardous Materials, 2014,277:34-43.
[11] 施嘉琛,胡建英,常红,等.北京温榆河流域耐药大肠杆菌的调查研究[J]. 中国环境科学, 2008,28(1):39-42. Shi J C, Hu J Y, Chang H, et al. Investigation on the antibiotic-resistance E.coli in Wenyu river in Beijing[J]. China Environment Science, 2008,28(1):39-42.
[12] 马奔,黄雅梦,王若楠,等.城市污水厂MCR-1基因及其携带菌的污染[J]. 中国环境科学, 2018,38(4):1433-1440. Ma B, Huang M D, Wang R N, et al. The pollution of MCR-1and MCR-1hosting bacteria in municipal wastewater treatment plants[J]. China Environment Science, 2018,38(4):1433-1440.
[13] A P Johnson, N Woodford, Global spread of antibiotic resistance:the example of New Delhi metallo-β-lactamase (NDM)-mediated carbapenem resistance[J]. J. Med. Microbiol, 2013,(62):499-513.
[14] 李建柱,侯杰,张鹏飞,等.空心菜浮床对鱼塘水质和微生物多样性的影响[J]. 中国环境科学, 2016,36(10):3071-3080. Li J Z, Hou J, Zhang P F, et al. Influence on water quality and microbial diversity in fish pond by Ipomoea aquatica floating-bed[J]. China Environment Science, 2016,36(10):3071-3080.
[15] A P, R P, H S, et al. Antibiotic resistance genes as emerging contaminants:studies in northern Colorado.[J]. Environmental Science & Technology, 2006,40(23):7445-50.
[16] Ben W, Wang J, Pan X, et al. Dissemination of antibiotic resistance genes and their potential removal by on-farm treatment processes in nine swine feedlots in Shandong Province, China[J]. Chemosphere, 2017,167:262-268.
[17] 2019~2024年中国b-内酰胺类抗生素行业市场供需及重点企业投资评估研究分析报告[R]. 中国报告大厅, 2019-06-09, http://www.chinabgao.com/report/4978993.html. 2019~2024 China's b-lactam antibiotics industry market supply and demand and key enterprise investment evaluation research analysis report[R]. China Report Hall, 2019-06-09,http://www.chinabgao.com/report/4978993.html.
[18] Antimicrobial resistance:global report on surveillance. In World Health Organization[R]. WHO, 2014.
[19] Yin X, Song F, Gong Y, et al. A systematic review of antibiotic utilization in China[J]. Journal of antimicrobial Chemotherapy, 2013,68(11):2445-2452.
[20] E L F. Clonality and antimicrobial resistance gene profiles of multidrug-resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil[J]. Journal of clinical microbiology, 2006,8(44).
[21] Fernández-Bravo A, Figueras M J. An update on the Genus Aeromonas:Taxonomy, epidemiology, and pathogenicity[J]. Microorganisms, 2020,8(1):129.
[22] 汪永禄,黄振洲,王利,等.2017年安徽省马鞍山市环境来源气单胞菌的分子特征和耐药分析[J]. 疾病监测, 2019,34(9):805-810. Wang Y L, Huang Z Z, Wang L, et al. Molecular characteristics and drug resistance of Aeromonas from environmental Sources in Ma'anshan, Anhui[J]. Disease Surveillance, 2019,34(9):805-810.
[23] Janda J M, Abbott S L The Genus Aeromonas:Taxonomy, pathogenicity, and infection[J]. Clinical Microbiology Reviews, 2010, 23(1):35-73.
[24] Mclellan S L, Huse S M, Muellerspitz, S. R., et al. Diversity and population structure of sewage-derived microorganisms in wastewater treatment plant influent[J]. Environmental Microbiology, 2010,12(2):378-392.
[25] Aljassim N, Ansari M I, Harb M, et al. Removal of bacterial contaminants and antibiotic resistance genes by conventional wastewater treatment processes in Saudi Arabia:Is the treated wastewater safe to reuse for agricultural irrigation?[J]. Water Research, 2015:277-290.
[26] 吴亚锋.南京地区气单胞菌的分离鉴定及嗜水气单胞菌菌株分型研究[D]. 南京:南京农业大学, 2015. Wu Y F. Isolation and identification of Aeromonas from Nanjing and typing of A.hydrophila strains[D]. Nanjing:Nanjing Agricultural University, 2015.
[27] Latif-Eugenín F, Beaz-Hidalgo R, Figueras M J. Evaluation of diﬀerent conditions and culture media for the recovery of Aeromonas spp. from water and shellfish samples[J]. Appl. Microbiol., 2016, 121:883-891.
[28] Pianetti A, Bruscolini F, Sabatini L, et al. Microbial characteristics of marine sediments in bathing area along Pesaro-Gabicce coast (Italy):A preliminary study[J]. Appl. Microbiol, 2004,97:682-689.
[29] Pessoa R B, De Oliveira W F, Marques D S, et al. The genus Aeromonas:A general approach[J]. Microbial Pathogenesis, 2019:81-94.
[30] Qiao M, Ying G G, Singer, A C, et al. Review of antibiotic resistance in China and its environment[J]. Environment international, 2018,110:160-172.
[31] Singer A C, Shaw H, Rhodes V, et al. Review of antimicrobial resistance in the environment and its relevance to environmental regulators[J]. Frontiers in microbiology, 2016,7:1728.
[32] Dang H, Ren J, Song L, et al. Dominant chloramphenicol-resistant bacteria and resistance genes in coastal marine waters of Jiaozhou Bay, China[J]. World Journal of Microbiology and Biotechnology, 2008, 24(2):209-217.
[33] Li D, Yu, T, Zhang Y, et al. Antibiotic resistance characteristics of environmental bacteria from an oxytetracycline production wastewater treatment plant and the receiving river[J]. Appl. Environ. Microbiol., 2010,76(11):3444-3451.
[34] Sun J, Hu J, Peng H, et al. Molecular and physiological characterization of fluoroquinolone resistance in relation to uropathogenicity among Escherichia coli isolates isolated from Wenyu River, China[J]. Chemosphere, 2012,87(1):37-42.
[35] Zou L K, Li, L W, Pan, X., et al. Molecular characterization of β-lactam-resistant Escherichia coli isolated from Fu River, China[J]. World Journal of Microbiology and Biotechnology, 2012,28(5):1891-1899.
[36] Piotrowska M, Popowska M. The prevalence of antibiotic resistance genes among Aeromonas species in aquatic environments[J]. Annals of microbiology, 2014,64(3):921-934.
[37] Vila J, Ruiz J, Gallardo F, et al. Aeromonas spp. and traveler's diarrhea:Clinical features and antimicrobial resistance[J]. Emerging Infectious Diseases, 2003,9(5):552.
[38] Aravena-Román M, Inglis T J, Henderson, B., et al. Antimicrobial susceptibilities of Aeromonas strains isolated from clinical and environmental sources to 26antimicrobial agents[J]. Antimicrobial agents and chemotherapy, 2012,56(2):1110-1112.
[39] Dias C, Serra C R, Simões L C, et al. Extended-spectrum β-lactamase and carbapenemase-producing Aeromonas species in wild animals from Portugal[J]. Veterinary Record, 2014, vetrec-2013.
[40] Codjoe F S, Donkor E S. Carbapenem resistance:a review[J]. Medical Sciences, 2018,6(1):1.
[41] Girlich D, Poirel L, Nordmann, P. PER-6, an extended-spectrum β-lactamase from Aeromonas allosaccharophila[J]. Antimicrobial agents and chemotherapy, 2010,54(4):1619-1622.
[42] Girlich D, Poirel, L, Nordmann P. Diversity of clavulanic acid-inhibited extended-spectrum β-lactamases in Aeromonas spp. from the Seine River, Paris, France[J]. Antimicrobial agents and chemotherapy, 2011,55(3):1256-1261.
[43] Maravić A, Skočibušić M, Šamanić I, et al, J. Aeromonas spp. simultaneously harbouring blaCTX-M-15, blaSHV-12, blaPER-1and blaFOX-2, in wild-growing Mediterranean mussel (Mytilus galloprovincialis) from Adriatic Sea, Croatia[J]. International journal of food microbiology, 2013,166(2):301-308.
[44] Blaak H, van Hoek A H, Veenman C, et al. Extended spectrum ß-lactamase-and constitutively AmpC-producing Enterobacteriaceae on fresh produce and in the agricultural environment[J]. International journal of food microbiology, 2014,168:8-16.
[45] Tacão M, Correia A, Henriques I. Resistance to broad-spectrum antibiotics in aquatic systems:anthropogenic activities modulate the dissemination of bla CTX-M-like genes[J]. Appl. Environ. Microbiol., 2012,78(12):4134-4140.
[46] Carvalho M J, Martínez-Murcia A, Esteves A C, et al. Phylogenetic diversity, antibiotic resistance and virulence traits of Aeromonas spp. from untreated waters for human consumption[J]. International journal of food microbiology, 2012,159(3):230-239.
[47] Henriques I S, Fonseca F, Alves A, et al. Occurrence and diversity of integrons and β-lactamase genes among ampicillin-resistant isolates from estuarine waters[J]. Research in microbiology, 2006,157(10):938-947.
[48] Gordon L, Cloeckaert A, Doublet B, et al. Complete sequence of the floR-carrying multiresistance plasmid pAB5S9 from freshwater Aeromonas bestiarum[J]. Journal of Antimicrobial Chemotherapy, 2008,62(1):65-71.