稻草秸秆发酵液对铜绿微囊藻生化参数影响

张庭廷; 胡春霞; 陈波

PDF(501 KB)

中国环境科学 ›› 2021, Vol. 41 ›› Issue (11) : 5345-5352.

环境生态

稻草秸秆发酵液对铜绿微囊藻生化参数影响

张庭廷^1,2, 胡春霞¹, 陈波²

作者信息 +

Effects of rice straw fermentation liquid on the biochemical parameters of Microcystis aeruginosa

ZHANG Ting-ting^1,2, HU Chun-xia¹, CHEN Bo²

Author information +

文章历史 +

摘要

针对铜绿微囊藻在稻草秸秆（水稻分蘖枝）发酵液胁迫下的酶学特征及抗氧化能力、藻毒素和多糖含量等生化指标进行了检测分析，旨在为利用稻草秸秆抑藻提供进一步理论依据.结果表明：铜绿微囊藻在水稻分蘖枝发酵液胁迫下，其表征藻细胞代谢水平的酯酶活性显著降低，实验第5d，最高浓度组（0.65%V/V）99.9%的藻细胞内酯酶活性均被抑制，超氧化物歧化酶（SOD）与谷胱甘肽过氧化物酶（GSH-Px）活力分别下降至同期对照组的11.03%和8.47%；丙二醛（MDA）含量则与发酵液浓度以及作用时间呈显著正相关，pearson分析表明，所有浓度组和作用时间内r值均大于0.9，而P值均小于0.01，表明水稻分蘖枝发酵液可显著降低藻细胞的抗氧化水平；但高浓度水稻分蘖枝发酵液没有引起微囊藻毒素（MCs）升高，甚至显著降低MCs和多糖的含量，与对照组相比，P<0.01.因此，水稻分蘖枝发酵液可通过影响铜绿微囊藻的代谢过程，降低藻细胞抗氧化以及抵御对环境胁迫的能力，从而达到有效抑藻的目的.

Abstract

The enzymatic characteristics, antioxidant capacity, microcystins and polysaccharide contents of Microcystis aeruginosa were investigated under the stress of rice straw (rice tillering branch) fermentation liquid, so as to provide a further theoretical basis for using rice straw to inhibit algae. The results showed that the esterase activity of M. aeruginosa under the stress of rice tiller branch fermentation liquid significantly reduced, which characterizes the level of algal cell metabolism. On the 5th day, 99.9% of the M. aeruginosa intracellular esterase activity was inhibited in the highest concentration group (0.65% V/V), the SOD and GSH-Px activities of the cells were only 11.03% and 8.47% of the control group in the same period. The MDA content was significantly positively correlated with the concentration of the fermentation liquid and the action time, pearson analysis showed that the r values were greater than 0.9 for all concentration groups, while the p values were less than 0.01, indicating that the rice tiller fermentation liquid can significantly reduce the antioxidant level of M. aeruginosa; but the high-concentration rice tiller fermentation liquid did not cause the increase of MCs, and even significantly reduce the content of MCs and polysaccharides, compared with the control group, P<0.01. Therefore, the fermentation liquid of rice tillering branches can affect the metabolic process of M.aeruginosa, and reduce the ability of the cells to resist oxidation and environmental stress, thereby it can achieve the purpose of effectively inhibiting M.aeruginosa

导出引用

张庭廷, 胡春霞, 陈波. 稻草秸秆发酵液对铜绿微囊藻生化参数影响[J]. 中国环境科学. 2021, 41(11): 5345-5352

ZHANG Ting-ting, HU Chun-xia, CHEN Bo. Effects of rice straw fermentation liquid on the biochemical parameters of Microcystis aeruginosa[J]. China Environmental Science. 2021, 41(11): 5345-5352

中图分类号： X52

参考文献

[1] Eladel H, Battah M, Dawa A, et al. Effect of rice straw extracts on growth of two phytoplankton isolated from a fish pond[J]. Journal of Applied Phycology, 2019,31:3557-3563.
[2] Eladel H, Abd-Elhay R, Anees D. Effect of rice straw application on water quality and microalgal flora in fish ponds[J]. Egyptian Journal of Botany, 2018,59:133-142.
[3] Hua Q, Liu Y G, Yan Z L, et al. Allelopathic effect of the rice straw aqueous extract on the growth of Microcystis aeruginosa[J]. Ecotoxicology and Environmental Safety, 2018,148:953-959.
[4] Elzaawely A A, Hanafey F M, El-Sayed M E A, et al. Phenolic compounds and antioxidant activity of rice straw extract[J]. International Letters of Natural Sciences, 2017,64:1-9.
[5] 胡春霞,陈波,张庭廷.稻草秸秆发酵液的抑藻效应及其机理研究[J]. 中国环境科学, 2021,41(4):1925-1931.Hu C X, Chen B, Zhang T T. Study on the algae inhibition effect and mechanism of the fermented liquid of rice straw[J]. China Environmental Science, 2021,41(4):1925-1931.
[6] Harke M J, Steffen M M, Gobler C J, et al. A review of the global ecology, genomics, and biogeography of the toxic cyanobacterium, Microcystis spp.[J]. Harmful Algae, 2016,54:4-20.
[7] He X, Liu Y L, Conklin A, et al. Toxic cyanobacteria and drinking water:Impacts, detection, and treatment[J]. Harmful Algae, 2016,54:174-193.
[8] Mecina G F, Dokkedal A L, Saldanha L L, et al. Response of Microcystis aeruginosa BCCUSP 232to barley (Hordeum vulgare L.) straw degradation extract and fractions[J]. Science of the Total Environment, 2017,599-600:1837-1847.
[9] Wang R, Xue Q N, Wang J T, et al. Effects of an allelochemical in Phaeodactylum tricornutum filtrate on Heterosigma akashiwo:Morphological, physiological and growth effects[J]. Chemosphere, 2017,186:527-534.
[10] 张庭廷,郑春艳,何梅,等.亚油酸对铜绿微囊藻的抑制机理[J]. 中国环境科学, 2009,29(4):419-424.Zhang T T, Zheng C Y, He M, et al. The inhibitory mechanism of linoleic acid on Microcystis aeruginosa[J]. China Environmental Science, 2009,29(4):419-424.
[11] Zhang T T, Wang L L, He Z X, et al. Growth inhibition and biochemical changes of cyanobacteria induced by emergent macrophyte Thalia dealbata roots[J]. Biochemical Systematics and Ecology, 2011,39:88-94.
[12] Meng P, Pei H, Hu W, et al. Allelopathic effects of Ailanthus altissima extracts on Microcystis aeruginosa growth, physiological changes and microcystins release[J]. Chemosphere, 2015,141:219-226.
[13] 文竹,闫浩,施媚,等.洋葱乳酸浸提液对铜绿微囊藻的抑制效应[J]. 卫生研究, 2018,47(5):822-826.Wen Z, Yan H, Shi M, et al. Inhibitory effect of the lactic acid leaching solution of Allium cepa L. on Microcystis aeruginosa[J]. Journal of hygiene research, 2018,47(5):822-826.
[14] Shen F, Wang L, Zhou Q, et al. Effects of lanthanum on Microcystis aeruginosa:Attention to the changes in composition and content of cellular microcystins[J]. Aquatic Toxicology, 2018,196:9-16.
[15] Li L, Jing X L, Wang L, et al. The extract of aquatic macrophyte Carex cinerascens induced colony formation in bloom-forming cyanobacterium Microcystis aeruginosa[J]. Environmental Science and Pollution Research, 2020,27:42276-42282.
[16] Li X, Dreher T W, Li R. An overview of diversity, occurrence, genetics and toxin production of bloom-forming Dolichospermum (Anabaena) species[J]. Harmful Algae, 2016,54:54-68.
[17] 肖溪.大麦秸秆对蓝藻化感抑制作用与机理的研究[D]. 杭州:浙江大学, 2012.Xiao X. Allelopathic inhibition of cyanobacteria by barley straw and its mechanism[D]. Zhejiang University, 2012.
[18] 黄爱缨,吴珍龄.水稻谷胱甘肽过氧化物酶的测定法[J]. 西南农业大学学报, 1999,21(4):1-4.Huang A Y, Wu Z L. Determination of glutathione peroxidase in rice seedlings[J]. Journal of Southwest Agricultural University, 1999, 21(4):1-4.
[19] Stewert R C, Bewely J D. Lipid peroxidation accociated with accelerated aging of soybean axes[J]. Plant physiol, 1980,65:245-248.
[20] Cavas L, Yurdakoc K, Yokes B. Antioxidant status of Lobiger serradifalci and Oxynoe olivacea (Opisthobranchia, Mollusca). J Exp Mar Biol Ecol. 2005,314:227-235.
[21] 张庭廷,郑春艳,何梅,等.脂肪酸类物质的抑藻效应及其构效关系[J]. 中国环境科学, 2009,28(3):274-279.Zhang T T, Zheng C Y, He M, et al. Inhibition on algae of fatty acids and the structure-effect relationship[J]. China Environmental Science, 2009,28(3):274-279.
[22] 彭桂莹,陈永玲,韩玉珍,等.乳酸对铜绿微囊藻的抑藻效应及机理[J]. 中国环境科学, 2016,36(4):1167-1172.Peng G Y, Chen Y L, Han Y Z, et al. The inhibitory effect of lactic acid on Microcystis aeruginosa and its mechanisms[J]. China Environmental Science, 2016,36(4):1167-1172.
[23] Kurmayer R, Deng L, Entfellner E. Role of toxic and bioactive secondary metabolites in colonization and bloom formation by filamentous cyanobacteria Planktothrix[J]. Harmful Algae, 2016,54:69-86.
[24] Cirés S, Ballot A. A review of the phylogeny, ecology and toxin production of bloom-forming Aphanizomenon spp. and related species within the Nostocales (cyanobacteria)[J]. Harmful Algae, 2016,54:21-43.
[25] Carmichael W W, Boyer G L. Health impacts from cyanobacteria harmful algae blooms:Implications for the North American Great Lakes[J]. Harmful Algae, 2016,54:194-212.
[26] He X, Liu Y L, Conklin A, et al. Toxic cyanobacteria and drinking water:Impacts, detection, and treatment[J]. Harmful Algae, 2016,54:174-193.
[27] Watson S B, Monis P, Baker P, et al. Biochemistry and genetics of taste- and odor-producing cyanobacteria[J]. Harmful Algae, 2016,54:112-127.
[28] Grattan L M, Holobaugh S, Morris J G. Harmful algal blooms and public health[J]. Harmful Algae, 2016,57:2-8.