Isolation and identification of an efficient algicidal bacteria strain and algicidal characteristics on Microcystis aeruginosa
HONG Gui-yun, MA Shao-xiong, WANG Jia, ZHANG Jin
Key Laboratory of Water Pollution Control and Waste Water Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China
An algicidal bacteria strain with a strong algicidal effect to M.aeruginosa was isolated from the eutrophic water. Then, the algicidal bacteria was identified as WJ6 by analyzing it's morphological features, physiological and biochemical characteristics, as well as phylogenetic analysis of 16S rDNA sequences. The algae-lysing means of WJ6 and the effects of different culture periods, concentrations of bacterium and environmental factors on the algae-lysing action were investigated to analyze the algicidal characteristics and possible algae-lysing mechanism of the strain WJ6. And the results showed that WJ6 was identified as Serratia sp.(Accession No.KY462187). The algicidal mechanism of WJ6 is indirectively, which disolves the algae mainly by an extracellular products. At logarithmic growth phase, the strain WJ6 can dissolve M. aeruginosa with a removal rate of 87.50%. The bacteria WJ6with on centration more than 1.4×109CFU/mL can give the highest removal rate of 95.69%. Under the condition of 30℃ and pH8, the removal rate is 90.00%. The effect of modified basic mediumon the algae-lysing effect of WJ6 is the most obvious with the higests removal rate of 98.50%. Furthermore, the removal rate of the algicidal bacterium is 98.33% when the ammonium ion concentration in the mediumis above 2mol/L. The removal rate is 92.77% when the salinity is 0.5%. All the results stated above indicate that the algicidal bacteria strain WJ6 have a strong algicidal effect to M. aeruginosa and prospective application in the control of M. aeruginosa in eutrophic water.
洪桂云, 马少雄, 王佳, 张瑾. 高效铜绿微囊藻溶藻菌WJ6的分离鉴定及溶藻特性[J]. 中国环境科学, 2018, 38(11): 4269-4275.
HONG Gui-yun, MA Shao-xiong, WANG Jia, ZHANG Jin. Isolation and identification of an efficient algicidal bacteria strain and algicidal characteristics on Microcystis aeruginosa. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(11): 4269-4275.
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