高原生境下A2O工艺对污水处理的微生物机制

郝凯越; 李远威; 宗永臣; 尤俊豪; 郭明哲

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中国环境科学 ›› 2021, Vol. 41 ›› Issue (5) : 2240-2251.

水污染与控制

高原生境下A²O工艺对污水处理的微生物机制

郝凯越, 李远威, 宗永臣, 尤俊豪, 郭明哲

作者信息 +

Microbial mechanism of A²O process for wastewater treatment in plateau habitat

HAO Kai-yue, LI Yuan-wei, ZONG Yong-chen, YOU Jun-hao, GUO Ming-zhe

Author information +

文章历史 +

摘要

基于Illumina MiSeq高通量测序技术，以高原生境下A²O工艺的活性污泥为研究对象，探讨不同温度下微生物群落结构、丰度及相关性.结合污染物分解、吸附及转化等代谢过程中的主要功能蛋白、功能基因、酶的种类及丰度在碳、氮、磷等污染物代谢途径中的作用，从生物化学和分子水平的角度分析了高原地区A²O工艺对污水处理的微生物学机制.结果表明：优势细菌门为变形菌门、拟杆菌门、放线菌门、厚壁菌门及绿弯菌门，优势细菌属为norank_f__AKYH767、腐败螺旋菌、Ottowia、伯克氏菌、IMCC26207、Novosphingobium.污染物去除和微生物群落发挥作用的效果在15℃为最优，微生物群落的COG、代谢途径、基因和酶活性最适宜的温度为20℃.主要功能蛋白为一般功能预测、氨基酸运输与代谢和基因转录；主要代谢途径为：ABC转运蛋白，细菌双组分调节系统，嘌呤、核糖体及嘧啶等代谢，主要功能基因的产物（酶）为：烯酰辅酶A水合酶、乙酰辅酶A C-乙酰转移酶、醛脱氢酶（NAD⁺）、硫代硫酸盐及外多磷酸酶.

Abstract

Based on Illumina MiSeq high-throughput sequencing technology, the structure, abundance and correlation of the microbial community at different temperatures were discussed in the A²O process activated sludge under the plateau habitat. Combined with the role of the main functional proteins, functional genes, enzymes and their abundance in the metabolic pathways of carbon, nitrogen, phosphorus, sulfur and other pollutants during the decomposition, adsorption and transformation of pollutants, the microbial mechanism of A²O process for sewage treatment in plateau area was analyzed from the perspective of biochemistry and molecular level. The results showed that the dominant bacterial Phylum were Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes and Chloroflexi, and the dominant bacterial genus norank_f_AKYH767, norank_f_Saprospiraceae, Ottowia, unclassified_f_Burkholderiaceae, IMCC26207, and Novosphingobium. The optimum temperature for pollutant removal and microbial community was 15℃, and the optimum temperature for COG, metabolic pathway, gene and enzyme activity of microbial community was 20℃. The main functional proteins were general function prediction only, amino acid transport and metabolism, transcription. The main metabolic pathways were ABC transporters, two-component system, purine metabolism, ribosome and pyrimidine metabolism. The main functional gene products (enzymes) were enoyl-CoA hydratase, acetyl-CoA C-acetyltransferase, aldehyde dehydrogenase (NAD+), thiosulfate and exopolyphosphatase.

导出引用

郝凯越, 李远威, 宗永臣, 尤俊豪, 郭明哲. 高原生境下A²O工艺对污水处理的微生物机制[J]. 中国环境科学. 2021, 41(5): 2240-2251

HAO Kai-yue, LI Yuan-wei, ZONG Yong-chen, YOU Jun-hao, GUO Ming-zhe. Microbial mechanism of A²O process for wastewater treatment in plateau habitat[J]. China Environmental Science. 2021, 41(5): 2240-2251

中图分类号： X703

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