Abstract：Based on Illumina MiSeq high-throughput sequencing technology, the structure, abundance and correlation of the microbial community at different temperatures were discussed in the A2O process activated sludge under the plateau habitat. Combined with the role of the main functional proteins, functional genes, enzymes and their abundance in the metabolic pathways of carbon, nitrogen, phosphorus, sulfur and other pollutants during the decomposition, adsorption and transformation of pollutants, the microbial mechanism of A2O process for sewage treatment in plateau area was analyzed from the perspective of biochemistry and molecular level. The results showed that the dominant bacterial Phylum were Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes and Chloroflexi, and the dominant bacterial genus norank_f_AKYH767, norank_f_Saprospiraceae, Ottowia, unclassified_f_Burkholderiaceae, IMCC26207, and Novosphingobium. The optimum temperature for pollutant removal and microbial community was 15℃, and the optimum temperature for COG, metabolic pathway, gene and enzyme activity of microbial community was 20℃. The main functional proteins were general function prediction only, amino acid transport and metabolism, transcription. The main metabolic pathways were ABC transporters, two-component system, purine metabolism, ribosome and pyrimidine metabolism. The main functional gene products (enzymes) were enoyl-CoA hydratase, acetyl-CoA C-acetyltransferase, aldehyde dehydrogenase (NAD+), thiosulfate and exopolyphosphatase.
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