Abstract：Polypropylene (PP) microplastics (MPs) were used to investigate their effects on the production of methane (CH4) and volatile fatty acids (VFAs) in sludge anaerobic digestion. The abundances of acetate kinase (AK) and mcrA genes under stresses of different amounts of PP MPs were determined by quantitative real-time PCR methods. The results showed that production of CH4 and VFAs was enhanced by addition of PP MPs. The cumulative productions of CH4 and acetic acid increased with increasing dosages of PP MPs. When the addition of PP MPs was 0.2g/g VSS, the cumulative productions of CH4 and acetic acid increased by 148.2% to 227.1mL/g and 15.2% to 1291.2mg/L, respectively, compared with the controls. Correspondingly, the abundance of mcrA genes increased by 98.2%, which indicated that PP MPs had a promoting effect on the growth of methanogens, thereby enhanced CH4production during sludge anaerobic digestion.
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