Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, Xi'an University of Architecture and Technology, Xi'an 710055, China
A laboratory study was conducted to investigate the effects of compost amendment on hydrocarbon degradation and microbial communities in petroleum contaminated soil. Petroleum hydrocarbons were analyzed using gravimetric method and GC-MS. Illumia Miseq technique was used to detect the microbial community structure of petroleum-polluted soil during the remediation. Results showed the removal efficiencies of total petroleum hydrocarbon (TPH), alkane, and polycyclic aromatic hydrocarbons (PAHs) were respectively (12.4±0.01)%, (10.2±0.01)%, and (9.38±0.02)% in the compost amendment soil (SC), compared to (3.21±0.02)%, (-3.00±0.01)%,and (-6.59±0.02)% in the control experiment (CK) after 42days of incubation. In response to compost amendment, the Shannon index, ACE index, and Chao1index increased from 4.30, 3489.3, 2691.0 to 5.80, 4684.7, and 3851.8, respectively. The relative abundance of Actinobacteria phyla decreased from 47.3% to 28.2%, and Bacteroidetes phyla increased from 0.78% to 16.2%. At the genus level, the relative abundance of the dominant genus in the petroleum contaminated soil including promicromonospora, Exiguobacterium, Nocardioides, Mycobacterium, and Citrobacter decreased significantly. Some new genus including Azomonas, Luteimonas, Pseudosphingo bacterium, and Parapedobacter appeared in the compost treated soil. The results indicated mature compost amendment effectively promoted petroleum hydrocarbon degradation in the soil, and soil microbial communities shifted significantly.
Wang X, Wang Q, Wang S, et al. Effect of biostimulation on community level physiological profiles of microorganisms in field-scale biopiles composed of aged oil sludge[J]. Bioresource Technology, 2012,111(3):308-315.
Gogoi B K, Dutta N N, Goswami P, et al. A case study of bioremediation of petroleum-hydrocarbon contaminated soil at a crude oil spill site[J]. Advances in Environmental Research, 2003,7(4):767-782.
[4]
刘五星,骆永明.土壤石油污染与生物修复[M]. 北京:科学出版社, 2015:166-170.
[5]
Shahi A, Aydin S, Ince B, et al. Evaluation of microbial population and functional genes during the bioremediation of petroleum-contaminated soil as an effective monitoring approach[J]. Ecotoxicology & Environmental Safety, 2016,125:153-160.
[6]
Leys N M, Bastiaens L, Verstraete W, et al. Influence of the carbon/nitrogen/phosphorus ratio on polycyclic aromatic hydrocarbon degradation by Mycobacterium and Sphingomonas in soil[J]. Applied Microbiology & Biotechnology, 2005,66(6):726-736.
[7]
Bento F M, Camargo F A, Okeke B C, et al. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation[J]. Bioresource Technology, 2005,96(9):1049-55.
Sayara T, Sarrà M, Sánchez A. Effects of compost stability and contaminant concentration on the bioremediation of PAHs-contaminated soil through composting[J]. Journal of Hazardous Materials, 2010,179(1):999-1006.
Wu M L, Dick W A, Li W, et al. Bioaugmentation and biostimulation of hydrocarbon degradation and the microbial community in a petroleum-contaminated soil[J]. International Biodeterioration & Biodegradation, 2016,107(3):158-164.
Wu M L, Li W, Dick W A, et al. Bioremediation of hydrocarbon degradation in a petroleum contaminated soil and microbial population and activity determination[J]. Chemosphere, 2017,169:124-130.
[19]
Dibble J T, Bartha R. Effect of environmental parameters on the biodegradation of oil sludge[J]. Applied & Environmental Microbiology, 1979,37(4):729-39.
[20]
Chaîneau C H, Rougeux G, Yéprémian C, et al. Effects of nutrient concentration on the biodegradation of crude oil and associated microbial populations in the soil[J]. Soil Biology & Biochemistry, 2005,37(8):1490-1497.
[21]
Yanto D H Y, Tachibana S. Enhanced biodegradation of asphalt in the presence of Tween surfactants, Mn2+, and H2O2, by Pestalotiopsis sp. in liquid medium and soil[J]. Chemosphere, 2014,103(5):105-113.
[22]
Lee S H, Lee S, Kim D Y, et al. Degradation characteristics of waste lubricants under different nutrient conditions[J]. Journal of Hazardous Materials, 2007,143(1):65-72.
[23]
Wu G, Kechavarzi C, Li X, et al. Influence of mature compost amendment on total and bioavailable polycyclic aromatic hydrocarbons in contaminated soils[J]. Chemosphere, 2013,90(8):2240-2246.
Li H, Ye D, Wang X, et al. Soil bacterial communities of different natural forest types in Northeast China[J]. Plant & Soil, 2014,383(1/2):203-216.
[26]
Sengupta A, Dick W A. Bacterial community diversity in soil under two tillage practices as determined by pyrosequencing[J]. Microbial Ecology, 2015,70(3):1-7.
Zhao J K, Li X M, Zhang M J, et al. Parapedobacter pyrenivorans sp. nov. isolated from a pyrene-degrading microbial enrichment, and emended description of the genus Parapedobacter[J]. International Journal of Systematic & Evolutionary Microbiology, 2013,63(Pt 11):3994-3999.
[30]
Pinyakong O, Habe H, Omori T. The unique aromatic catabolic genes in sphingomonads degrading polycyclic aromatic hydrocarbons (PAHs)[J]. Journal of General & Applied Microbiology, 2003,49(1):1-19.
[31]
Zhou D P, Xia Y, Han R Y, et al. Isolation, identification and degradation characteristics of three phenanthrene-degrading bacteria[J]. Acta Scientiae Circumstantiae, 2003,23(1):124-128.
