Effects of [C8mim]Cl on apoptosis and endoplasmic reticulum stress pathway in HepG2 cells
ZHANG Bang-jun1, TU Zhen-peng1, FENG Yi-yi1, LIU Yang1,2, LIXiao-yu1
1. College of Life Science, Henan Normal University, Xinxiang 453007, China; 2. Journal of Henan Normal University, Henan Normal University, Xinxiang 453007, China
Abstract:In order to explore whether ionic liquid 1-octyl-3-methylimidazolium chloride ([C8mim]Cl) can induce apoptosis through endoplasmic reticulum stress(ERS) pathway, HepG2 cells were treated with 0,50,100,200μmol/L [C8mim]Cl for 24h based on the MTT cell viability assay. The apoptosis was detected by flow cytometry and western blot was used to assay the expressions of ERS related proteins. The results showed that [C8mim]Cl increased the apoptosis in HepG2 cells with a concentration-dependent manner. [C8mim]Cl significantly increased the expressions of ERS related proteins, including glucose regulated protein 78 (GRP78), phosphorylated protein kinase RNA-like endoplasmic reticulum kinase (p-PERK), phosphorylated eukaryotic initiation factor 2α (p-eIF2α), phosphorylated inositol-requiring enzyme 1 (p-IRE1), activating transcription factor 4 (ATF4) and ATF6. [C8mim]Cl also significantly induced the expressions of C/EBP homologous protein (CHOP) and caspase 4 protein, lead to the increase of caspase 9 and caspase 3 activities. Therefore, [C8mim]Cl could induce apoptosis in HepG2 cells through ERS pathway.
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