南昌市PM2.5中结合氨基酸的浓度组成及来源

朱慧晓; 朱仁果; 程丽琴; 潘媛媛; 鲁承绍; 吴智辉; 李静雯

PDF(858 KB)

中国环境科学 ›› 2022, Vol. 42 ›› Issue (10) : 4509-4516.

大气污染与控制

南昌市PM_2.5中结合氨基酸的浓度组成及来源

朱慧晓^1,2, 朱仁果^1,2, 程丽琴^1,2, 潘媛媛^1,2, 鲁承绍¹, 吴智辉¹, 李静雯¹

作者信息 +

Concentration composition and source of combined amino acids in PM_2.5 in Nanchang city

ZHU Hui-xiao^1,2, ZHU Ren-guo^1,2, CHENG Li-qin^1,2, PAN Yuan-yuan^1,2, LU Cheng-shao¹, WU Zhi-hui¹, LI Jing-wen¹

Author information +

文章历史 +

摘要

采集了春季南昌市城市和森林地区的大气PM_2.5样品及潜在大气氨基酸排放源（植物和土壤）样品，测定并分析了样品中结合氨基酸（CAAs）的浓度、组成及甘氨酸（Gly）的氮同位素值.结果表明，城市和森林地区PM_2.5中总CAAs的浓度分别为0.507~3.912和0.497~2.647nmol/m³.通过对PM_2.5中CAAs组成占比分析发现，城市和森林地区CAAs组成成分相似，其中Pro、Gly、Ala、Leu和His是PM_2.5中丰富的CAAs物种.结合Gly的氮同位素值可知，城市地区（+0.62‰~+22.67‰）和森林地区（+1.99‰~+23‰） PM_2.5中δ¹⁵N_C_-Gly值表现出较大的变化范围.根据大气CAAs潜在排放源δ¹⁵N值清单，本研究中生物质燃烧、土壤源和植物源是PM_2.5中CAAs的主要来源.贝叶斯稳定同位素模型计算源分配结果表明生物质燃烧、土壤源和植物源对城市地区PM_2.5中CAAs的平均贡献为42%、40%和18%，对森林地区PM_2.5中CAAs的平均贡献为38%、38%和24%.

Abstract

Fine particulate matter (PM_2.5) and potential atmospheric amino acid emission sources (including plants and soil) samples were collected from the urban and forest areas of Nanchang city in spring. The concentration of combined amino acids (CAAs) and the nitrogen isotope of glycine (Gly) were analyzed. The results showed that the concentration of total CAAs ranged from 0.507 to 3.912 and from 0.497 to 2.647 nmol/m³ in the urban and forest areas, respectively. The composition profiles of CAAs in the urban and forest were similar. Among CAAs, Pro, Gly, Ala, Leu and His were the most abundant species in PM_2.5. The δ¹⁵N_C_-_Glyvalue in PM_2.5 collected in both the urban (ranged from+0.62‰ to +22.67‰) and forest (ranged from +1.99‰ to +23‰) sites exhibited a broad range in this study. According to the δ¹⁵N inventories of potential emission sources of atmospheric combined Gly, biomass burning, soil source and plant source were the main sources of combined Gly in this study. Furthermore, the results of the Bayesian Stable Isotope model showed the biomass burning, soil source and plant source average contributed 42%, 40% and 18% to the total CAAs in PM_2.5 in the urban and contributed 38%, 38% and 24% to the total CAAs in PM_2.5 in the forest site.

导出引用

朱慧晓, 朱仁果, 程丽琴, 潘媛媛, 鲁承绍, 吴智辉, 李静雯. 南昌市PM_2.5中结合氨基酸的浓度组成及来源[J]. 中国环境科学. 2022, 42(10): 4509-4516

ZHU Hui-xiao, ZHU Ren-guo, CHENG Li-qin, PAN Yuan-yuan, LU Cheng-shao, WU Zhi-hui, LI Jing-wen. Concentration composition and source of combined amino acids in PM_2.5 in Nanchang city[J]. China Environmental Science. 2022, 42(10): 4509-4516

中图分类号： X513

参考文献

[1] Scalabrin E, Zangrando R, Barbaro E, et al.Amino acids in arctic aerosols[J].Atmospheric Chemistry and Physics, 2012,12(21):10453-10463.
[2] Matos J T, Duarte R M, Duarte A C.Challenges in the identification and characterization of free amino acids and proteinaceous compounds in atmospheric aerosols:a critical review[J].TrAC Trends in Analytical Chemistry, 2016,75:97-107.
[3] Cape J N, Cornell S E, Jickells T D, et al.Organic nitrogen in the atmosphere-Where does it come from? a review of sources and methods[J].Atmospheric Research, 2011,102(1/2):30-48.
[4] Mikhailov E, Vlasenko S, Niessner R, et al.Interaction of aerosol particles composed of protein and saltswith water vapor:hygroscopic growth and microstructural rearrangement[J].Atmospheric Chemistry and Physics, 2004,4(2):323-350.
[5] Szyrmer W, Zawadzki I.Biogenic and anthropogenic sources of ice-forming nuclei:a review[J].Bulletin of the American Meteorological Society, 1997,78:209.
[6] Zhang Q, Anastasio C, Jimenez-Cruz M.Water-soluble organic nitrogen in atmospheric fine particles (PM_2.5) from Northern California[J].Journal of Geophysical Research:Atmospheres, 2002, 107(D11):AAC 3-1-AAC 3-9.
[7] Kristensson A, Rosenørn T, Bilde M.Cloud droplet activation of amino acid aerosol particles[J].The Journal of Physical Chemistry A, 2010,114(1):379-386.
[8] Chan M N, Choi M Y, Ng N L, et al.Hygroscopicity of water-soluble organic compounds in atmospheric aerosols:amino acids and biomass burning derived organic species[J].Environmental Science & Technology, 2005,39:1555-1562.
[9] Miguel A G, Cass G R, Glovsky M M, et al.Allergens in paved road dust and airborne particles[J].Environmental Science & Technology, 1999,33(23):4159-4168.
[10] Bianco A, Voyard G, Deguillaume L, et al.Improving the characterization of dissolved organic carbon in cloud water:amino acids and their impact on the oxidant capacity[J].Scientific Reports, 2016,6(1):1-7.
[11] Mandalakis M, Apostolaki M, Tziaras T, et al.Free and combined amino acids in marine background atmospheric aerosols over the Eastern Mediterranean[J].Atmospheric Environment, 2011,45(4):1003-1009.
[12] Song T, Wang S, Zhang Y, et al.Proteins and amino acids in fine particulate matter in rural Guangzhou, Southern China:seasonal cycles, sources, and atmospheric processes[J].Environmental Science & Technology, 2017,51(12):6773-6781.
[13] Milne P J, Zika R G.Amino acid nitrogen in atmospheric aerosols:occurrence, sources and photochemical modification[J].Journal of Atmospheric Chemistry, 1993,16:361-398.
[14] Di Filippo P, Pomata D, Riccardi C, et al.Free and combined amino acids in size-segregated atmospheric aerosol samples[J].Atmospheric Environment, 2014,98(98):179-189.
[15] Zhu R, Xiao H Y, Lv Z, et al.Nitrogen isotopic composition of free Gly in aerosols at a forest site[J].Atmospheric Environment, 2020, 222.
[16] Abe R Y, Akutsu Y, Kagemoto H.Protein amino acids as markers for biological sources in urban aerosols[J].Environmental Chemistry Letters, 2016,14(1):155-161.
[17] Liu X Y, Xiao H Y, Liu C Q, et al.Tissue N content and 15N natural abundance in epilithic mosses for indicating atmospheric N deposition in the Guiyang area, SW China[J].Applied Geochemistry, 2008,23(9):2708-2715.
[18] Xiao H Y, Tang C G, Xiao H W, et al.Mosses indicating atmospheric nitrogen deposition and sources in the Yangtze River Drainage Basin, China[J].Journal of Geophysical Research, 2010,115(D14).
[19] Anastasio C, Mcgregor K G.Photodestruction of dissolved organic nitrogen species in fog waters[J].Aerosol Science and Technology, 2000,32(2):106-119.
[20] Barbaro E, Feltracco M, Cesari D, et al.Characterization of the water soluble fraction in ultrafine, fine, and coarse atmospheric aerosol[J].Science of the Total Environment, 2019,658:1423-1439.
[21] Zhu R, Xiao H Y, Zhu Y, et al.Sources and transformation processes of proteinaceous matter and free amino acids in PM_2.5[J].Journal of Geophysical Research:Atmospheres, 2020,125:e2020JD032375.
[22] Ren L, Bai H, Yu X, et al.Molecular composition and seasonal variation of amino acids in urban aerosols from Beijing, China[J].Atmospheric Research, 2018,203:28-35.
[23] Zhu R, Xiao H Y, Zhang Z, et al.Compound-specific δ¹⁵N composition of free amino acids in moss as indicators of atmospheric nitrogen sources[J].Scientific reports, 2018,8(1):14347.
[24] 陈泽宪,徐辉碧.蛋白质水解阶段对氨基酸组成分析的影响[J].分析科学学报, 2002,18(1):80-85.Chen Z X, Xu H B.Effects of hydrolysis on amino acid composition analysis of proteins[J].Journal of Analytical Science, 2002,18(1):80-85.
[25] 丁雅韵,谢孟峡,康娟.蛋白质水解液中氨基酸组成的定量分析[J].分析化学, 2002,30(4):418-421.Ding Y Y, Xie M X, Kang J.Quantitative analysis of amino acids in protein hydrolysates[J].Chinese, Journal of Analytical Chemistry, 2002,30(4):418-421.
[26] Xu Y, Wu D, Xiao H, et al.Dissolved hydrolyzed amino acids in precipitation in suburban Guiyang, Southwestern China:seasonal variations and potential atmospheric processes[J].Atmospheric Environment, 2019,211:247-255.
[27] Wedyan M A, Preston M R.The coupling of surface seawater organic nitrogen and the marine aerosol as inferred from enantiomer-specific amino acid analysis[J].Atmospheric Environment, 2008,42(37):8698-8705.
[28] 朱玉雯,朱仁果,方小珍,等.森林地区PM_2.5中氨基酸的水平、来源及转化[J].中国环境科学, 2021,41(1):81-90.Zhu Y W, Zhu R G, Fang X Z, et al.The levels, sources and atmospheric photochemical processes of amino acids in PM_2.5 in forest areas[J].China Environmental Science, 2021,41(1):81-90.
[29] Kang H, Xie Z, Hu Q.Ambient protein concentration in PM₁₀ in Hefei, central China[J].Atmospheric Environment, 2012,54:73-79.
[30] 石金辉,范得国,韩静,等.青岛大气气溶胶中氨基化合物的分布特征[J].环境科学, 2010,31(11):2547-2554.Shi J H, Fan D G, Han J, et al.Distributions of dissolved and particulate amino compounds in atmospheric aerosols at Qingdao[J].Environmental Science, 2010,31(11):2547-2554.
[31] 朱济奇,孙文文,冯加良.上海大气PM_2.5中水溶性氨基酸的浓度及组成特征[J].生态环境学报, 2020,29(6):1173-1180.Zhu J Q, Sun W W, Feng J L.Concentration and composition of water-soluble amino acids in PM_2.5 in Shanghai[J].Ecology and Environmental Sciences, 2020,29(6):1173-1180.
[32] Matsumoto K, Kim S, Hirai A.Origins of free and combined amino acids in the aerosols at an inland urban site in Japan[J].Atmospheric Environment, 2021,259:118543.
[33] Matsumoto K, Uematsu M.Free amino acids in marine aerosols over the western North Pacific Ocean[J].Atmospheric Environment, 2005, 39(11):2163-2170.
[34] Pommie C, Levadoux S, Sabatier R, et al.IMGT standardized criteria for statistical analysis of immunoglobulin V-REGION amino acid properties[J].Journal of Molecular Recognition, 2004,17(1):17-32.
[35] Milne P J, Zika R G.Amino acid nitrogen in atmospheric aerosols:occurrence, sources and photochemical modification[J].Journal of Atmospheric Chemistry, 1993,16(4):361-398.
[36] Sharma V K, Graham N J D.Oxidation of amino acids, peptides and proteins by ozone:a review[J].Ozone:Science & Engineering, 2010, 32(2):81-90.
[37] Staton S J R, Woodward A, Castillo J A, et al.Ground level environmental protein concentrations in various ecuadorian environments:potential uses of aerosolized protein for ecological research[J].Ecological Indicators, 2015,48:389-395.