Effects of typical antibiotics on denitrifying anaerobic methane oxidation in the wetland
YANG Yu-qi1,2,3, ZHAO Yue-wen1,3,4, ZHANG Qiu-lan2, LIU Chang-li1,3, WEN Meng-tuo2,3
1. Fujian Provincial Key Laboratory of Water Cycling and Eco-Geological Processes, Xiamen 361021, China; 2. School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China; 3. Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang 050061, China; 4. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China
Abstract：The Baiyangdian wetland, with a high methane emission flux and heavily contaminated with quinolone antibiotics (QNs), was selected as the study area. The short-term effects of typical QNs including Ofloxacin (OFL), Norfloxacin (NOR), and Flumequine (FLU) on denitrifying anaerobic methane oxidation (DAMO) processes in Baiyangdian wetland was investigated by conducting 13C-labeled microcosm experiments. The results showed that: All three QNs promoted the microbial DAMO process, and the promotion effect on DAMO was OFL > NOR > FLU; Except for 2ng/g OFL, which had a negligible effect on the DAMO reaction rate, additional concentrations (20, 200, 2000ng/g) all promoted the DAMO processes in Baiyangdian wetlands, and the promotion showed a dose effect in general; Further analysis of the effects of QNs on the nitrite-DAMO driven by NO2- and the nitrite-DAMO driven by NO3- respectively, it could be found that 200ng/g NOR inhibited the bacterial-driven DAMO process with NO2- as electron acceptor (nitrite-DAMO), but promoted to a greater extent the archaea-driven DAMO process with NO3- as electron acceptor (nitrate-DAMO), ultimately showing a facilitation effect of QNs on the overall DAMO process driven by both NO3- and NO2-.
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