Dose-dependent joint resistance effects of antibacterial agents on Escherichia coli
TONG Dan-qing, SUN Hao-yu, ZHANG Yu-lian, WANG Jia-jun, TANG Liang, WU Ming-hong
Key Laboratory of Organic Compound Pollution Control Engineering(MOE), School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
Abstract：Three kinds of common antibacterial agents, i.e., sulfonamide potentiators (SAPs), silver nanoparticles (AgNPs), and sulfonamides (SAs), were selected to investigate joint resistance effects of SAPs-AgNPs and SAPs-AgNPs-SAs on Escherichia coli (E. coli). In addition, mutation unit (MU) method and plasmid conjugative transfer unit (CTU) method were used to judge the joint resistance actions of these antibacterial mixtures at different concentrations. The results show that 3 groups of SAPs-AgNPs and 9 groups of SAPs-AgNPs-SAs had the dose-dependent joint resistance effects: the joint actions of antibacterial mixtures on promoting the mutation frequency of E. coli were mainly antagonism, and their joint actions on promoting the plasmid conjugative transfer frequency of E. coli were changed from antagonism to synergism with an increase in concentration. These effects were speculated to relate to action mechanisms of the test antibacterial agents. Compared with the single antibacterial agent, SAPs-AgNPs and SAPs-AgNPs-SAs may reduce the resistance risk at low concentrations, and thus lower the emergence risk of antibiotic resistance genes (ARGs) but increase the dissemination risk of ARGs at high concentrations.
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