“鸡尾酒”式混合抗生素对细菌耐药性的影响

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摘要选择四环素、磺胺、磺胺增效剂3类常用抗生素, 以其对大肠杆菌(E. coli)的单一兴奋效应(hormesis)最大促进效应对应浓度(HC_max)配制等HC_max比的二元及三元混合体系, 探究混合体系对细菌生长和耐药性(突变、质粒接合转移)的联合效应.结果表明, 二元混合抗生素对E. coli生长和接合转移频率起到协同抑制, 对E. coli的突变频率起协同刺激; 三元混合抗菌剂对这三种效应均协同抑制.因此, 推测二元混合体系会导致毒性和耐药性风险同时增加; 三元混合体系会导致毒性风险增加、耐药性风险降低.

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	韩宝苍
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关键词 ：混合抗生素, 细菌耐药性, hormesis, 突变, 接合转移

Abstract：Three kinds of commonly used antibiotics, i.e., tetracyclines, sulfonamides, and sulfonamide potentiators, were selected as representatives to obtain their concentration for the hormetic maximum promoting effect on Escherichia coli (E. coli) (HC_max). The binary and ternary mixtures of test antibiotics were designed based on equal HC_max rations, and their combined effects on bacterial growth and resistance (mutation and plasmid conjugation) of E. coli were explored. The results showed binary mixtures synergistically inhibited the growth and conjugation frequency, but synergistically stimulated the mutation frequency. The combined effects of ternary mixtures on toxicity and resistance were all synergistic inhibition. Therefore, it could be speculated that binary mixtures might increase the risk of toxicity and resistance, while ternary mixtures might result in an increased toxicity risk but a decreased resistance risk.

Key words： antibiotic mixtures bacterial resistance hormesis mutation conjugation transfer

收稿日期: 2021-06-04

PACS:

X171.5

基金资助:国家自然科学基金资助项目(22006116, 21777123);博士后创新人才支持计划(BX20190247);中国博士后科学基金(2019M661624);上海“超级博士后”激励计划(2019194)

通讯作者: 孙昊宇, 副研究员, sunhaoyu2021@shu.edu.cn E-mail: sunhaoyu2021@shu.edu.cn

作者简介: 韩宝苍(1997-), 男, 山东临沂人, 同济大学博士研究生, 主要从事抗生素环境毒理研究.发表论文2篇

引用本文:

韩宝苍, 陈志彬, 张跃恒, 林志芬, 孙昊宇. “鸡尾酒”式混合抗生素对细菌耐药性的影响[J]. 中国环境科学, 2022, 42(1): 434-443. HAN Bao-cang, CHEN Zhi-bin, ZHANG Yue-heng, LIN Zhi-fen, SUN Hao-yu. The effects of antibiotic mixtures on bacterial resistance based on “cocktail” exposure. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(1): 434-443.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2022/V42/I1/434

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