Joint effects of antibiotics and silver nanoparticles on resistance of Escherichia coli
LI Xu-fei1,2, WU Xiao-dan3, SUN Hao-yu1,2, LIN Zhi-fen1,2
1. College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China;
2. Shanghai Institute of Pollution Control and Ecological Security, Tongji University, Shanghai 200092, China;
3. College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China
The effects of sulfonamides (SAs) and silver nanoparticles (AgNPs) on plasmid RP4 conjugative transfer and mutation were investigated using Escherichia coli (E. coli) as model organism. And the combined action modes of the two antibacterial agents were judged. Based on molecular docking and linear regression, the mechanism of the two effects and their relationship with toxicity were discussed. The results showed that the tested 12 SAs and AgNPs could promote conjugative transfer and mutation in the low concentration range whether under single or combined exposure to E. coli. Among the 12 groups of SAs-AgNPs mixtures, the maximum and minimum peak values of conjugative transfer frequency promotion rate were 105.32% and 46.96%, respectively, and the maximum and minimum peak values of mutant promotion rate were 1410.25% and 238.38%, respectively. The joint effects of SAs and AgNPs to plasmid RP4 conjugative transfer and mutation effect were mainly synergistic and antagonistic, respectively. Furthermore, there was a good correlation among conjugative transfer effect, mutation effect and toxicity effect. This paper provides a reference for the study of the emergence and dissemination of bacterial resistance.
李旭飞, 巫晓丹, 孙昊宇, 林志芬. 抗生素和纳米银对大肠杆菌耐药性的联合效应[J]. 中国环境科学, 2020, 40(11): 5045-5054.
LI Xu-fei, WU Xiao-dan, SUN Hao-yu, LIN Zhi-fen. Joint effects of antibiotics and silver nanoparticles on resistance of Escherichia coli. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(11): 5045-5054.
Lee S H, Jun B H. Silver nanoparticles:Synthesis and application for nanomedicine[J]. International Journal of Molecular Sciences, 2019, 20(4):865.
Kumar S, Shukla A, Baul P P, et al. Biodegradable hybrid nanocomposites of chitosan/gelatin and silver nanoparticles for active food packaging applications[J]. Food Packaging and Shelf Life, 2018,16:178-184.
Hebeish A, Naggar M E E, Fouda M M G, et al. Highly effective antibacterial textiles containing green synthesized silver nanoparticles[J]. Carbohydrate Polymers, 2011,86(2):936-940.
Ahmad S, Subhani K, Rasheed A, et al. Development of conductive fabrics by using silver nanoparticles for electronic applications[J]. Journal of Electronic Materials, 2020,49(2):1330-1337.
Garcia I, Lovell E C, Wong R J, et al. Silver-based plasmonic catalysts for carbon dioxide reduction[J]. ACS Sustainable Chemistry & Engineering, 2020,8(4):1879-1887.
Wegener H C. Antibiotics in animal feed and their role in resistance development[J]. Current Opinion in Microbiology, 2003,6(5):439-445.
Cerqueira F, Matamoros V, Bayona J M, et al. Antibiotic resistance gene distribution in agricultural fields and crops:A soil-to-food analysis[J]. Environmental Research, 2019,177:108608.
Zhang Y, Xie J, Liu M, et al. Microbial community functional structure in response to antibiotics in pharmaceutical wastewater treatment systems[J]. Water Research, 2013,47(16):6298-6308.
赖晓琳,吴平霄,阮博.四环素对大肠杆菌抗生素抗性基因进化的影响[J]. 环境科学学报, 2019,39(8):2475-2482. Lai X L, Wu P X, Ruan B. 2019. Evolution of antibiotic resistance genes in E. coli by tetracycline[J]. Acta Scientiae Circumstantiae, 2019,39(8):2475-248.
Paná?ek A, Kvítek L, Smékalová M, et al. Bacterial resistance to silver nanoparticles and how to overcome it[J]. Nature Nanotechnology, 2018,13:65-71.
周虹,王大力,葛鸿铭,等.磺胺与金属氧化物纳米颗粒对发光菌的急性联合毒性及其机制初探[J]. 生态毒理学报, 2016,11(5):65-70. Zhou H, Wang D L, Ge H M, et al. Acute joint effects and mechanisms of binary toxicity of sulfonamides and metal oxide nanoparticles to Vibrio fischeri[J]. Asian Journal of Ecotoxicology, 2016,11(5):65-70.
Wang D L, Wu X D, Lin Z F, et al. A comparative study on the binary and ternary mixture toxicity of antibiotics towards three bacteria based on QSAR investigation[J]. Environmental Research, 2018,162:127-134.
Mcgillicuddy E, Murray I, Kavanagh S, et al. Silver nanoparticles in the environment:Sources, detection and ecotoxicology[J]. Science of the Total Environment, 2017,575:231-246.
Pratsinis A, Hervella P, Leroux J C, et al. Toxicity of silver nanoparticles in macrophages[J]. Small, 2013,9(15):2576-2584.
Martinez, J L, Baquero F. Mutation frequencies and antibiotic resistance[J]. Antimicrobial Agents and Chemotherapy, 2000,44(7):1771-1777.
Jain R, Rivera M C, Lake J A. Horizontal gene transfer among genomes:The complexity hypothesis[J]. Proceedings of the National Academy of Sciences of the United States of America, 1999,96(7):3801-3806.
Davison J. Genetic exchange between bacteria in the environment[J]. Plasmid, 1999,42(2):73-91.
Salyers A A, Shoemaker N B. Broad host range gene transfer:Plasmids and conjugative transposons[J]. FEMS Microbiology Ecology, 1994,15(1/2):15-22.
Broderius S J, Kahl M D, Hoglund M D. Use of joint toxic response to define the primary-mode of toxic action for diverse industrial organic-chemicals[J]. Environmental Toxicology and Chemistry, 1995,14(9):1591-1605.
Long X, Wang D L, Lin Z F, et al. The mixture toxicity of environmental contaminants containing sulfonamides and other antibiotics in Escherichia coli:Differences in both the special target proteins of individual chemicals and their effective combined concentration[J]. Chemosphere, 2016,158:193-203.
Golbraikh A, Tropsha A. Beware of q2[J]. Journal of Molecular Graphics and Modelling, 2002,20(4):269-276.
Hitchings G H. Mechanism of action of trimethoprim-sulfamethoxazole[J]. Journal of Infectious Diseases, 1973,128(3):433-436.
Beaber J W, Kumar E, Sillanpaa M. SOS response promotes horizontal dissemination of antibiotic resistance genes[J]. Nature, 2004,427(6969):72-74.
伍玲丽,张晓雪,舒昆慧,等.两种粒径纳米银对Nitrosomonas europaea的毒性效应[J]. 中国环境科学, 2019,39(10):4401-4408. Wu L L, Zhang X X, Shu K H, et al. Toxicity of two sizes of silver nanoparticles to Nitrosomonas europaea[J]. China Environmental Science, 2019,39(10):4401-4408.
Kim S, Ryu D Y. Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues[J]. Journal of Applied Toxicology, 2013,33(2):78-89.
袁艺.银在医药方面的应用[J]. 中华中西医学杂志, 2005,3(8):11-12. Yuan Y. Application of silver in medicine[J]. China and Foreign Medical Journal, 2005,3(8):11-12.
Feng Q L, Wu J, Chen G Q, et al. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus[J]. Journal of Biomedical Materials Research, 2000,52(4):662-668.