磁铁矿作用下<i>Dv</i>H阴极生物膜的响应及转录组分析

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摘要 This study investigated the response mechanism of the biofilm by constructing a Desulfovibrio vulgaris Hildenborough (DvH) biocathode, under the influence of magnetite nanoparticles (MNPs). The results showed that the conversion rate of SO₄^2- to S^2- of the biocathode mediated by MNPs increased from 6.8% to 37.9%, compared to the group without MNPs. The results of the linear sweep voltammetry test revealed that, within the potential range of 0~-0.81V, the cathodic current observed in the group with MNPs was higher than that of the control group, suggesting that MNPs enhance the electrocatalytic activity of the cathodic biofilm. The comparative transcriptome analysis of DvH cathodic biofilm revealed that MNPs can facilitate hydrogen utilization in DvH by upregulating genes encoding [FeFe] hydrogenase, Hmc, and ATP synthase. In addition, genes encoding sulfate adenylyltransferase and adenylylsulphate reductase, both crucial enzymes in sulfur metabolism, were upregulated with MNPs addition, which contributed to an improved conversion rate of SO₄^2--S^2-. MNPs upregulated genes related to Flp/Tad pili assembly and PEP-CTERM protein, thereby augmenting the adhesion of DvH and promoting biofilm formation on the cathode. These findings contribute to a novel theoretical framework for the development of highly efficient microbial cathodic electrochemical systems.

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	滕敏
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	骆海萍

关键词 ：微生物电化学系统, 硫酸盐还原菌, 生物阴极, 纳米磁铁矿, 转录组学

Abstract：This study investigated the response mechanism of the biofilm by constructing a Desulfovibrio vulgaris Hildenborough (DvH) biocathode, under the influence of magnetite nanoparticles (MNPs). The results showed that the conversion rate of SO₄^2- to S^2- of the biocathode mediated by MNPs increased from 6.8% to 37.9%, compared to the group without MNPs. The results of the linear sweep voltammetry test revealed that, within the potential range of 0~-0.81V, the cathodic current observed in the group with MNPs was higher than that of the control group, suggesting that MNPs enhance the electrocatalytic activity of the cathodic biofilm. The comparative transcriptome analysis of DvH cathodic biofilm revealed that MNPs can facilitate hydrogen utilization in DvH by upregulating genes encoding [FeFe] hydrogenase, Hmc, and ATP synthase. In addition, genes encoding sulfate adenylyltransferase and adenylylsulphate reductase, both crucial enzymes in sulfur metabolism, were upregulated with MNPs addition, which contributed to an improved conversion rate of SO₄^2--S^2-. MNPs upregulated genes related to Flp/Tad pili assembly and PEP-CTERM protein, thereby augmenting the adhesion of DvH and promoting biofilm formation on the cathode. These findings contribute to a novel theoretical framework for the development of highly efficient microbial cathodic electrochemical systems.

Key words： microbial electrochemical system (MES) sulfate reducing bacteria biocathode magnetite nanoparticles transcriptomic

收稿日期: 2024-04-03

PACS:

X703.1

基金资助:国家自然科学基金资助项目(42077286);广东省自然科学基金资助项目(2023A1515011846)

通讯作者: 骆海萍,副教授,luohp5@mail.sysu.edu.cn E-mail: luohp5@mail.sysu.edu.cn

作者简介: 滕敏(1998-),女,湖南怀化人,中山大学硕士研究生,主要研究方向为微生物电化学.tengm3@mail2.sysu.edu.cn.

引用本文:

滕敏, 朱曦, 曾翠平, 胡佳萍, 刘广立, 骆海萍. 磁铁矿作用下DvH阴极生物膜的响应及转录组分析[J]. 中国环境科学, 2024, 44(11): 6088-6095. TENG Min, ZHU Xi, ZENG Cui-ping, HU Jia-ping, LIU Guang-li, LUO Hai-ping. Response and transcriptional analysis of DvH cathode biofilm with the effect of magnetite. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(11): 6088-6095.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I11/6088

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