Abstract：The behavior and biotoxic effects of two silver nanoparticles (AgNPs) modified by polyethyleneimine (BPEI-AgNPs with positive charge) and citric acid (Citrate-AgNPs with negative charge) on Microcystis aeruginosa (M. aeruginosa) were investigated to evaluate the surface charge-dependent biotoxicity of AgNPs on M. aeruginosa. A more pronounced suppressive influence on the growth and photosynthetic activity of M. aeruginosa was observed with the Citrate-AgNPs than with the BPEI-AgNPs. The peak content of malondialdehyde (MDA) in 0.5mg/L Citrate-AgNP group (100.8nmol/mgprot) was higher than that in the same concentration BPEI-AgNP group (82.5nmol/mgprot). Compared with BPEI-AgNPs, Citrate-AgNPs demonstrated a significant up- and down-regulation on sod2 and GPX gene expression in M. aeruginosa, resulting in a higher peroxidation in algal cells. The extracellular polymer (EPS) secretion was observed with BPEI-AgNPs, amounting up to 32.21mg/gSS in 0.5mg/L BPEI-AgNP group (16d). Subsequently, the toxicity of BPEI-AgNPs was neutralized by the coating effect of EPS. The final intracellular Ag+ released in the 0.5mg/L Citrate-AgNP group was 2.13 times that of the 0.5mg/L BPEI-AgNP group, and M. aeruginosa cells were seriously ruptured. Therefore, the surge of Ag+ ions triggered severe cellular impairment to cells by Citrate-AgNPs. Furthermore, Citrate-AgNPs significantly inhibited the expression of photosynthesis (rbcS and rbcL) and energy metabolism (por) genes.
Carmichael, Wayne W.Health effects of toxin-producing cyanobacteria:‘The CyanoHABs’ [J].Human & Ecological Risk Assessment, 2001,7(5):1393-1407.
[2]
Gao H, Zhao Z, Zhang L, et al.Cyanopeptides restriction and degradation co-mediate microbiota assembly during a freshwater cyanobacterial harmful algal bloom (CyanoHAB) [J].Water Research, 2022,220:118674.
[3]
Sultana S, Awal S, Shaika N A, et al.Cyanobacterial blooms in earthen aquaculture ponds and their impact on fisheries and human health in Bangladesh [J].Aquaculture Research, 2022,53(15):5129-5141.
[4]
Qian Z, Cao Y, Wang L, et al.Developing cyanobacterial bloom predictive models using influential factor discrimination approach for eutrophic shallow lakes [J].Ecological Indicators, 2022,144:109458.
[5]
Franklin N M, Rogers N J, Apte S C, et al.Comparative toxicity of nanoparticulate ZnO, Bulk ZnO, and ZnCl2 to a freshwater microalga (Pseudokirchneriella subcapitata):The importance of particle solubility [J].Environmental Science & Technology, 2007,41(24):8484-8490.
[6]
Hou J, Yang Y, Wang P, et al.Effects of CeO2, CuO, and ZnO nanoparticles on physiological features of Microcystis aeruginosa and the production and composition of extracellular polymeric substances [J].Environmental Science and Pollution Research, 2017,24(1):226-235.
[7]
Nekvapil F, Bunge A, Tudoran L B, et al.Single-cell Raman micro-spectroscopy for tracking of carotenoids in cyanobacteria exposed to Mn and Zn doped ferrite nanoparticles [J].Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, 2021,254(F4):119607.
[8]
Wu D, Zhang J, Du W, et al.Toxicity mechanism of cerium oxide nanoparticles on cyanobacteria Microcystis aeruginosa and their ecological risks [J].Environmental Science and Pollution Research, 2022,29(23):34010-34018.
[9]
Fabrega J, Luoma S N, Tyler C R, et al.Silver nanoparticles:Behaviour and effects in the aquatic environment [J].Environment International, 2011,37(2):517-531.
[10]
Mariano S, Panzarini E, Inverno M D, et al.Toxicity, bioaccumulation and biotransformation of glucose-capped silver nanoparticles in green microalgae Chlorella vulgaris [J].Nanomaterials, 2020,10(7):1377.
[11]
Singh A, Hou W C, Lin T F.Combined impact of silver nanoparticles and chlorine on the cell integrity and toxin release of Microcystis aeruginosa [J].Chemosphere, 2021,272:129825.
[12]
Romero N, Visentini F F, Márquez V E, et al.Physiological and morphological responses of green microalgae Chlorella vulgaris to silver nanoparticles [J].Environmental Research, 2020,189:109857.
[13]
Zhang J, Xiang Q, Shen L, et al.Surface charge-dependent bioaccumulation dynamics of silver nanoparticles in freshwater algae [J].Chemosphere, 2020,247:125936.
[14]
Zhang J L, Zhou Z P, Pei Y, et al.Metabolic profiling of silver nanoparticle toxicity in Microcystis aeruginosa [J].Environmental Science:Nano, 2018,5(11):2519-2530.
[15]
Silva T, Pokhrel L R, Dubey B, et al.Particle size, surface charge and concentration dependent ecotoxicity of three organo-coated silver nanoparticles:Comparison between general linear model-predicted and observed toxicity [J].Science of the Total Environment, 2014, 468-469:968-976.
[16]
Badawy A M E, Luxton T P, Silva R G, et al.Impact of environmental conditions (pH, ionic strength, and electrolyte type) on the surface charge and aggregation of silver nanoparticles suspensions [J].Environmental Science & Technology, 2010,44(4):1260-1266.
[17]
周为.水环境中银纳米颗粒早期聚集动力学与长期演变机制研究[D].武汉:武汉大学, 2018.Zhou W.Research on early aggregation kinetics and long-term fate mechanism of AgNPs in aquatic environment [D].Wuhan:Wuhan University, 2018.
[18]
Reinsch B C, Levard C, Li Z, et al.Sulfidation of silver nanoparticles decreases escherichia coli growth inhibition [J].Environmental Science & Technology, 2012,46(13):6992-7000.
[19]
Levard C, Hotze E M, Lowry G V, et al.Environmental transformations of silver nanoparticles:Impact on stability and toxicity [J].Environmental Science & Technology, 2012,46(13):6900.
[20]
El Badawy A M, Silva R G, Morris B, et al.Surface charge-dependent toxicity of silver nanoparticles [J].Environmental Science & Technology, 2010,45(1):283-287.
[21]
Pham T L.Effect of silver nanoparticles on tropical freshwater and marine microalgae [J].Journal of Chemistry, 2019,2019:1-7.
[22]
Huang J, Cheng J, Yi J.Impact of silver nanoparticles on marine diatom Skeletonema costatum [J].Journal of Applied Toxicology, 2016,36(10):1343-1354.
[23]
Oukarroum A, Bras S, Perreault F O, et al.Inhibitory effects of silver nanoparticles in two green algae, Chlorella vulgaris and Dunaliella tertiolecta [J].Ecotoxicology & Environmental Safety, 2012,78(none):80-85.
[24]
Zhang C, Ling F, Yi Y L, et al.Algicidal activity and potential mechanisms of ginkgolic acids isolated from Ginkgo biloba exocarp on Microcystis aeruginosa [J].Journal of Applied Phycology, 2014, 26(1):323-332.
[25]
Nam S H, An Y J.Cell size and the blockage of electron transfer in photosynthesis:Proposed endpoints for algal assays and its application to soil alga Chlorococcum infusionum [J].Chemosphere, 2015,128:85-95.
[26]
Borgstahl G E O, Oberley-Deegan R E.Superoxide dismutases (SODs) and SOD mimetics [J].Antioxidants (Basel), 2018,7(11):156.
[27]
于朋飞,刘晖,牟之健,等.纳米银和银离子对2种微藻的急性毒性效应[J].生态毒理学报, 2017,12(6):188-198.Yu P F, Liu H, Mou Z J, et al.Acute toxic effects of silver nanoparticles and silver ion on two microalgae [J].Asian Journal of Ecotoxicology, 2017,12(6):188-198.
[28]
Perreault F, Bogdan N, Morin M, et al.Interaction of gold nanoglycodendrimers with algal cells (Chlamydomonas reinhardtii) and their effect on physiological processes [J].Nanotoxicology, 2012, 6(2):109-120.
[29]
Navarro E, Piccapietra F, Wagner B, et al.Toxicity of silver nanoparticles to Chlamydomonas reinhardtii [J].Environmental Science & Technology, 2008,42(23):8959-8964.
[30]
Hwang E T, Lee J H, Chae Y J, et al.Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria [J].Small, 2008,4(6):746-50.
[31]
Razzak M A, Lee D W, Yoo Y J, et al.Evolution of rubisco complex small subunit transit peptides from algae to plants [J].Scientific Reports, 2017,7(1):9279.
[32]
Fukuzawa H, Fujiwara S, Tachiki A, et al.Nucleotide sequences of two genes CAH1 and CAH2 which encode carbonic anhydrase polypeptides in Chlamydomonas reinhardtii [J].Nucleic Acids Research, 1990,18(21):6441-6442.
