Analysis of microbial communities and environmental factors in sewer sediments
HUANG Shuai-chen, ZUO Jian-e, CHEN Lei, SHENG Zi-qiong, LI Yu-qing, ZHANGA Yu, WANG Mo-ran, LIU Yan-chen
State Key Joint Laboratory of Environmental Simulation and Pollution Control(SKLESPC), School of Environment, Tsinghua University, Beijing 100084, China
Abstract：Sewer sediments were sampled in a district of Beijing and analyzed using high-throughput sequencing, it was found that Proteobacteria, Euryarchaeota, Firmicutes, Bacteroidetes were the dominant phyla, and Deltaproteobacteria, Methanomicrobia, Clostridia, Bacteroidia were the dominant classes of all samples. The functional microorganisms Sulfate-Reducing Bacteria (SRB) and Methanogenic Archaea (MA) were prevalent in sewer sediments at the genus level. Among the six sewer pipes sampled, the relative abundances of MA were 20.6% and 40.8% in the sewer pipes S3 and S4, higher than other sewer pipes, which implied higher the methane production potential. Thus there was a risk of combustible gas accumulation in S3 and S4. The relative abundances of SRB were 9.14% and 8.19% in the sewer pipes S5 and S6, higher than other sewer pipes, which implied that the potential of sulfate reduced to sulfide was greater. Thus there was a risk of sewer corrosion in S5 and S6. Redundancy Analysis (RDA) denoted that the dissolved oxygen, temperature, sulfate, total nitrogen of sewage had correlation with the microbial communities in the sewer pipe sediments.
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