Mechanism of tetracycline degradation by soil microbial electrochemical systems
ZHAO Xiao-dong1,2, LI Xiao-jing1, ZHAO Peng-yu2, SONG Min-li2, YAN Ping-mei2, REN Tian-zhi1, LI Yong-tao1,3
1. Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China; 2. Department of Biology, Taiyuan Normal University, Yuci 030619, China; 3. College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China
Abstract:A soil microbial electrochemical system (SMES) was constructed to study tetracycline degradation, and the samples were collected after 58 days of incubation under dark and constant temperature conditions. The relationships among tetracycline degradation, soil physicochemical properties, enzyme activity and three-domain microorganisms were analyzed to reveal the biodegradation mechanism of tetracycline in the SMES. The results showed that the degradation rate of tetracycline was significantly increased to 70% from 52% by using the SMES. Compared with the control treatment, the activity of dehydrogenase in the SMES increased by 144%, and it was significantly positively correlated with tetracycline degradation. In contrast, weak tetracycline degradation by polyphenol oxidase, catalase and laccase was observed in the SMES. As an important physicochemical factor affecting soil microbial community, soil pH value was negatively correlated with tetracycline degradation. Network analysis showed that fungi played a key role in the degradation of tetracycline, the genera of Geoalkalicharacter, Microascus, Wardomyces and Scopulariopsis were potential degrading microorganisms of tetracycline, among which Microascus and Scopulariopsis may secrete dehydrogenase to degrade tetracycline.
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