Abstract:Apple stock-Pingyitiancha (Malus hupehensis Rehd) was selected as experimental materials, hydroponic experiments were conducted with or without fluoranthene and benzo (b) fluoranthene. Non-invasive Micro-test Technique was used to detect the effects of Ca2+, K+, H+ fluxes in response to polycyclic aromatic hydrocarbons (PAHs) treatments. Our results showed that: (1) fluoranthene and benzo (b) fluoranthene treatments resulted in the liquidity of Ca2+increased from(-63.53±9.30)pmol/(cm2×s) to (+62.85±10.00)pmol/(cm2×s)、(91.33±19.72) pmol/(cm2×s); the liquidity of K+ increased from(-60.56±14.56)pmol/(cm2×s) to(+32.60±5.44)pmol/(cm2×s)、(+36.76±5.23)pmol/(cm2×s); the liquidity of H+reduced from(+44.38±5.19)pmol/(cm2×s) to(-0.72±0.055)pmol/(cm2×s)、(-6.34±0.79)pmol/(cm2×s). fluoranthene and benzo (b) fluoranthene treatments resulted in the liquidity of Ca2+, K+, H+ in root epidermis obvious reversal. A dramatic Ca2+, K+ efflux was observed in root epidermis under stress, and gradually reduced to a stable level. Meanwhile, H+ showed influx and did not affect the PAHs treatments. (2) Benzo[b]fluoranthene Stress showed more toxic effects on ion mobility in root hair cells than that of fluoranthene. The results indicated that PAHs stress could damage the ion mobility of root hair cells, interfere plants growth and development, providing a theoretical basis for further study on the response of plants to PAHs stress.
徐金, 黄翠香, 刘青, 毛云飞, 倪伟, 于文章, 沈向. 平邑甜茶根毛细胞离子流动性对PAHs胁迫的响应[J]. 中国环境科学, 2016, 36(10): 3107-3111.
XU Jin, HUANG Cui-xiang, LiU Qing, MAO Yun-fei, NI Wei, YU Wen-zhang, SHEN Xiang. The ion mobility in root hair cells of Malus hupehensis in response to stress from PAHs. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(10): 3107-3111.
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