细菌对城市污水中斜生栅藻生长与产脂的影响

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摘要

研究并分析了EM菌和LAS菌对斜生栅藻（Scenedesmus obliquus）生长和油脂积累的促进作用.结果表明：添加上述2种细菌对城市污水中斜生栅藻的干重和油脂产量有显著的促进作用，其油脂产量分别提高了36.2%和21.5%.通过对生成的脂肪酸甲酯进行气相色谱分析，结果显示EM菌的添加提升了斜生栅藻单不饱和脂肪酸的含量，有利于提升所得生物柴油的质量.培养结束后污水的菌群结构分析显示投加细菌会增加污水中菌群的丰富度和多样性，且相比于对照组，加菌后的试验组中β-Proteobacteria、α-Proteobacteria丰度有所提高，而拟杆菌门（Bacteroidetes）丰度有所降低.

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	韩松芳
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	涂仁杰
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	陈洪一

关键词 ：斜生栅藻, 城市污水, 细菌, 产脂

Abstract：

In the study, EM bacteria and LAS bacteria were found to enhance the growth and lipid production of Scenedesmus obliquus cultivated in municipal wastewater. The results showed that the lipid production of S. obliquus was increased by 36.2% and 21.5% after adding the above-mentioned bacteria, respectively. According to the GC analysis of the lipids, EM bacteria could increase the content of monounsaturated fatty acid in S. obliquus, and thus improving the grade of biodiesel. The analysis of microbial community structure in municipal wastewater showed that the richness and diversity of bacteria in wastewater were increased significantly. In addition, the abundances of β-Proteobacteria、α-Proteobacteria were increased, while the abundance of Bacteroidetes was decreased.

Key words： Scenedesmus obliquus municipal wastewater bacteria lipid production

收稿日期: 2017-03-22

PACS:

X172

基金资助:

深圳市科技计划资助项目（JCYJ20150529114024234）

通讯作者: 金文标,教授,jinwb@hit.edu.cn E-mail: jinwb@hit.edu.cn

作者简介: 韩松芳(1989-),女,河南周口人,哈尔滨工业大学深圳研究生院博士研究生,主要从事微藻生物质能源与污水资源化研究.

引用本文:

韩松芳, 金文标, 涂仁杰, 周旭, 陈洪一. 细菌对城市污水中斜生栅藻生长与产脂的影响[J]. 中国环境科学, 2017, 37(10): 3867-3872. HAN Song-fang, JIN Wen-biao, TU Ren-jie, ZHOU Xu, CHEN Hong-yi. Effects of bacteria on growth and lipid production of Scenedesmus obliquus cultivated in municipal wastewater. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(10): 3867-3872.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2017/V37/I10/3867

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