Abstract：NaClO, H2O2, CuSO4 and diuron are four commonly used chemical algaecides at present. All of them can inactivate algaes and control algae blooms based on different mechanisms. However, algae-derived endotoxins can be released during inactivation of Microcystis aeruginosa. The effects of these algaecides on the release of endotoxins were evaluated and compared, and the following results were obtained:Endotoxins of 1564.78, 872.14, and 852.03 EU/mL were released after inactivation of algae by 0.10~0.50mg/L NaClO, while 732.23, 1706.22, and 944.84 EU/mL endotoxins were detected when treated by 0.10~0.50mmol/L H2O2. When the algae was inactivated by 0.5~2.5μmol/L CuSO4, 854.60, 1055.45, and 1513.15EU/mL endotoxins were found in water. Endotoxins of 1802.23, 1788.11, and 1886.14 EU/mL were released when 5~25μmol/L diuron was used for inactivation of algae. Algaecides with oxidizing ability can degrade part of endotoxins in solution, while algaecides without oxidizing ability cannot control and degrade the releasing endotoxins efficiently. However, the concentrations of endotoxins under all aforementioned conditions were far greater than that produced via normal metabolism (113.86EU/mL). Therefore, even though the algae in algae-laden water were controlled, it is also necessary to avoid the direct contact with the water to reduce the exposure of endotoxins.
卜令君, 周石庆, 施周, 王涛, 衣启航, 孙聚龙. 藻源性内毒素在铜绿微囊藻灭活过程中的释放[J]. 中国环境科学, 2017, 37(12): 4675-4680.
BU Ling-jun, ZHOU Shi-qing, SHI Zhou, WANG Tao, YI Qi-hang, SUN Ju-long. Release of algae-derived endotoxins during inactivation of Microcystis aeruginosa. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(12): 4675-4680.
Burford M A, Johnson S A, Cook A J, et al. Correlations between watershed and reservoir characteristics, and algal blooms in subtropical reservoirs[J]. Water Research, 2007,41(18):4105-4114.
Li L, Gao N, Deng Y, et al. Characterization of intracellular & extracellular algae organic matters (AOM) of Microcystic aeruginosa and formation of AOM-associated disinfection byproducts and odor & taste compounds[J]. Water Research, 2012,46(4):1233-1240.
[4]
Zhou S, Shao Y, Gao N, et al. Effects of different algaecides on the photosynthetic capacity, cell integrity and microcystin-LR release of Microcystis aeruginosa[J]. Science of the Total Environment, 2013,463:111-119.
[5]
Jan?ula D, Maršálek B. Critical review of actually available chemical compounds for prevention and management of cyanobacterial blooms[J]. Chemosphere, 2011,85(9):1415-1422.
He X, Zhang G, de la Cruz A A, et al. Degradation mechanism of cyanobacterial toxin cylindrospermopsin by hydroxyl radicals in homogeneous UV/H2O2 process[J]. Environmental Science & Technology, 2014,48(8):4495-4504.
[8]
Anderson W B, Slawson R M, Mayfield C I. Review/Synthèse A review of drinking-water-associated endotoxin, including potential routes of human exposure[J]. Canadian Journal of Microbiology, 2002,48(7):567-587.
Parikh S J, Chorover J. Infrared spectroscopy studies of cation effects on lipopolysaccharides in aqueous solution[J]. Colloids & Surfaces B Biointerfaces, 2007,55(2):241-250.
Elin R J, Wolff S M, McAdam K, et al. Properties of reference Escherichia coli endotoxin and its phthalylated derivative in humans[J]. Journal of Infectious Diseases, 1981,144(4):329-336.
[13]
Xue J, Zhang J, Xu B, et al. Endotoxins:The critical risk factor in reclaimed water via inhalation exposure[J]. Environmental Science & Technology, 2016,50(21):11957-11964.
Rapala J, Lahti K, Räsänen L A, et al. Endotoxins associated with cyanobacteria and their removal during drinking water treatment[J]. Water Research, 2002,36(10):2627-2635.
[16]
Zhang C, Liu W, Wen S, et al. Endotoxin contamination and control in surface water sources and a drinking water treatment plant in Beijing, China[J]. Water Research, 2013,47(11):3591-3599.
[17]
Anderson W B, Mayfield C I, Dixon D G, et al. Endotoxin inactivation by selected drinking water treatment oxidants[J]. Water Research, 2003,37(19):4553.
[18]
Anderson W B, Huck P M, Dixon D G, et al. Endotoxin Inactivation in Water by Using Medium-Pressure UV Lamps[J]. Applied & Environmental Microbiology, 2003,69(5):3002-3004.
[19]
Qian H, Yu S, Sun Z, et al. Effects of copper sulfate, hydrogen peroxide and N-phenyl-2-naphthylamine on oxidative stress and the expression of genes involved photosynthesis and microcystin disposition in Microcystis aeruginosa[J]. Aquatic Toxicology, 2010,99(3):405-412.
[20]
Gaikowski M P, Rach J J, Ramsay R T. Acute toxicity of hydrogen peroxide treatments to selected lifestages of cold-, cool-, and warmwater fish[J]. Aquaculture, 1999,178(3):191-207.
[21]
Verhoeven R L, Eloff J N. Effect of lethal concentrations of copper on the ultrastructure and growth of Microcystis[J]. Proceedings-Southern African Electron Microscopy Society, 1979,9:161-162.
[22]
Giacomazzi S, Cochet N. Environmental impact of diuron transformation:a review[J]. Chemosphere, 2004,56(11):1021-1032.
[23]
Daly R I, Ho L, Brookes J D. Effect of chlorination on Microcystis aeruginosa cell integrity and subsequent microcystin release and degradation[J]. Environmental Science & Technology, 2007,41:4447-4453.
