1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou Jiangsu 215009, China;
2. Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material, Suzhou Jiangsu 215009, China;
3. Key Laboratory of Environmental Science and Engineering of Jiangsu Province, Suzhou Jiangsu 215009, China
The effects of influent ammonium loading rate (ALR) on the species abundance of microbial communities and dominant bacterial in the ABR-MBR combined process were investigated by Miseq high-throughput sequencing. The results indicated that the numbers of ammonium oxidizing bacteria (AOB) can be significantly increased and the bio-activities of nitrite oxidizing bacteria (NOB) can be inhibited at the temperature of 28~32℃, pH of 7.1~7.4 and DO of 0.5~1mg/L by gradually increasing the influent ALR in the MBR. So that the shortcut nitrification will be achieved efficiently and stably. When influent ALR was 0.94kg/(m3·d), the average nitrite accumulation rate was above 60%, and NH4+-N removal rate was 90%. Proteobacteria were the dominant bacterial. The relative abundance of Nitrosomonas was increased from 4.97% to 22.56%, the relative abundance of Nitrospira was increased from 0.06% to 2.12% during the operation. Therefore, the nitrite accumulation rates were closely related to the bio-activities and abundance of AOB of shortcut nitrification the process. Its showed that shortcut nitrification can be efficiently achieved by a large number of AOB growth. Still, a slight increase of NOB abundance had little effect on the realization of shortcut nitrification. The microbial diversity and its functional structure stability were the guarantee of a stable and high removal efficiency in the ABR-MBR combined process.
Ciudad G, Munoz O R, Ruiz G, et al. Partial nitrification of high ammonia concentration wastewater as a part of a shortcut biological nitrogen removal process. Process Biochem[J]. Process Biochemistry, 2005,40(5):1715-1719.
[2]
Wang C C, Lee P H, Kumar M, et al. Simultaneous partial nitrification, anaerobic ammonium oxidation and denitrification (SNAD) in a full-scale landfill-leachate treatment plant.[J]. Journal of Hazardous Materials, 2010,175(1-3):622-628.
[3]
Limpiyakorn T, Shinohara Y, Kurisu F, et al. Communities of ammonia-oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo[J]. Fems Microbiology Ecology, 2005,54(2):205-217.
[4]
Criddle C, Wells G. Community analysis of ammonia-oxidizing bacteria in activated sludge of eight wastewater treatment systems[J]. Journal of Environmental Sciences, 2010,22(4):627-634.
Hallin S, Lydmark P, Kokalj S, et al. Community survey of ammonia-oxidizing bacteria in full-scale activated sludge processes with different solids retention time[J]. Journal of Applied Microbiology, 2005,99(3):629-640.
Guo J, Peng Y, Wang S, et al. Long-term effect of dissolved oxygen on partial nitrification performance and microbial community structure.[J]. Bioresource Technology, 2009,100(11):2796-2802.
[9]
Ciudad G, González R, Bornhardt C, et al. Modes of operation and pH control as enhancement factors for partial nitrification with oxygen transport limitation.[J]. Water Research, 2007, 41(20):4621-4629.
Turk O, Mavinic D S. Benefits of using selective inhibition to remove nitrogen from highly nitrogenous wastes[J]. Environmental Technology, 1987,8(1-12):419-426.
Pace N R. A molecular view of microbial diversity and the biosphere[J]. Science, 1997,276(5313):734-740.
[15]
Lyautey E, Lacoste B N D, Ten-Hage L C, et al. Analysis of bacterial diversity in river biofilms using 16S rDNA PCR-DGGE:methodological settings and fingerprints interpretation[J]. Water Research, 2005,39(2/3):380-388.
[16]
Wu P, Ji X, Song X, et al. Nutrient removal performance and microbial community analysis of a combined ABR-MBR (CAMBR) process[J]. Chemical Engineering Journal, 2013, 232(9):273-279.
[17]
López-Gutiérrez J C, Henry S, Hallet S, et al. Quantification of a novel group of nitrate-reducing bacteria in the environment by real-time PCR[J]. Journal of Microbiological Methods, 2004, 57(3):399-407.
Jubany I, Lafuente J, Baeza J A, et al. Total and stable washout of nitrite oxidizing bacteria from a nitrifying continuous activated sludge system using automatic control based on Oxygen Uptake Rate measurements.[J]. Water Research, 2009,43(11):2761-2772.
Chang Y M, Yang Q, Hao C B, et al.[Experimental study of autotrophic denitrification bacteria through bioaugmentation of activated sludge from municipal wastewater plant].[J]. Environmental Science, 2011,32(4):1210-1216.
Kragelund C, Levantesi C, Borger A. Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants[J]. Microbiology, 2008,154(Pt 3):886-894.
[26]
Yang C, Zhang W, Liu R, et al. Phylogenetic diversity and metabolic potential of activated sludge microbial communities in full-scale wastewater treatment plants.[J]. Environmental Science & Technology, 2011,45(17):7408-7415.
[27]
Park H D, Noguera D R. Nitrospira community composition in nitrifying reactors operated with two different dissolved oxygen levels[J]. Journal of Microbiology & Biotechnology, 2008,18(8):1470-1474.
[28]
Burrell P, Keller J, Blackall L L. Characterisation of the bacterial consortium involved in nitrite oxidation in activated sludge[J]. Water Science & Technology, 1999,39(6):45-52.
[29]
Thomsen T R, Kong Y, Nielsen P H. Ecophysiology of abundant denitrifying bacteria in activated sludge[J]. Fems Microbiology Ecology, 2007,60(3):370-382.