1. Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China;
2. Beijing Fengtai District Health Inspection, Beijing 100071, China
Inthis research, a new system that combination of the ion exchangemembrane and ultrafiltration membrane (IEM-UF) nitrogen enrichment with shortcut nitrification and denitrification was proposed for low carbon nitrogen ratio of domestic sewage. The performance of nitrogen and COD removalin the system were studiedunder the three stage operating condition. Meanwhile, the characteristicsof the microbial community in the system were analyzed by high throughput technology, andthe effect of the flora changes on the nitrogen removal was investigated. The rate of nitrite accumulation in the shortcut nitrification reactor reached above 90%only 19d when C/N was 3and DO=0.5mg/L.When the ratio of denitrifying influent was 2:1, the average removal rates of COD and NOx--Nreached above 80% and 89%, respectively. The maximum removal rate of TN was above 64.8%. Besides, the change of bacterial community structure in the three stage was consistent with the change of nitrogen removal efficiency. Under the three operatingconditions, the proportion of Nitrosomonas accounted for the proportion of the genus in the shortcut nitrification reactor was about 3.69%, 5.48% and 0.53% respectively. As well asthe sum of denitrifying bacteria Dechloromonas and Thauera in the denitrification reactor accounted for 33.35%, 25.62% and 20.52%, respectivelyunder the three stage.
Carrera J, Vicent T, Lafuente J. Effect of Influent COD/N Ratio On Biological Nitrogen Removal (BNR) FromHigh-Strength Ammonium Industrial Wastewater[J]. Process Biochemistry, 2004,39(12):2035-2041.
Tokutomi T. Operation of a nitrite-type airlift reactor at low DO concentration[J]. Wat. Sci. Technol., 2004,49(5/6):81-88.
[13]
Balmelle B. Study of factors controlling nitrite build-up inbiological processes of water nitrification[J]. Wat.Sci. Technol., 1992,26(5/6):1017-1025.
[14]
Yoo H, Ahn K-H, Lee H-J, et al. Nitrogen removal from syntheticwastewater by simultaneous nitrification and denitrification vianitrite in an intermittently-aerated reactor[J]. Wat. Res., 1999,33(1):145-154.
Utaker J B, Nes I F. A qualitative evaluation of the published oligo nucledtides specific for the 16S rRNA gene sequences of ammonia oxidizing bacteria[J]. Syst Appl Microbiol, 1998,21(1):72-88.
Horn M A, Ihssen J, Matthies C, et al. Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa.[J]. International journal of systematic and evolutionary microbiology, 2005,55(3):1255.
[27]
Coates J D, Chakraborty R, Lack J G, et al. Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of Dechloromonas[J]. Nature, 2011,411(6841):1039-43.
[28]
Zhou H X, Li X K, Chu Z R, et al. Effect of temperature downshifts on a bench-scale hybrid A/O system:Process performance and microbial community dynamics[J]. Chemosphere, 2016,153:500-507.