Microbial community structure characteristics and its key driving factors in surface sediments along Nanfei River
YANG Chang-ming1, WU Ya-qiong1, WANG Yu-lai2, GUO Yun-hao1
1. Key Laboratory of Yangtze Water Environment of Ministry of Education, Tongji University, Shanghai 200092, China;
2. School of Energy and Environment, Anhui University of Technology, Maanshan 234000, China
In order to explore the differences in bacterial community structure and its driving factors in the sediment of Nanfei River, the key physiochemical parameters including pH, CEC, TOC, DOC, and various forms of nitrogen were determined in the surface sediment samples along Nanfei River with different land uses such as urban, suburb, industrial park and rural areas. Specially, sediment bacterial community structure characteristics were analyzed by using MiSeq high-throughput sequencing technology. The relationships between sediment bacterial community structure and aforementioned physiochemical parameters were examined by redundancy analysis (RDA). The results showed that the major bacterial phylum is Proteobacteria in the surface sediments, followed by Chloroflexi, Bacteroidetes, Firmicutes. At the level of class, the major bacterial class are Anaerolineae, Gammaproteobacteria, Clostridia, Deltaproteobacteria besides the unclassified. Epsilonproteobacteria were detected mainly in sediment samples from downstream of the Nanfei River, suggesting that they were not ubiquitous in the sediment ecosystem of the Nanfei River. Most of the genus is unclassified, with Longilinea and Bellilinea being predominant species. Different types of land uses along the Nanfei River led to different pollution sources and discharging load will result in different physicochemical properties of surface sediment, which in turn reshape the bacterial community structure of the sediment. The bacterial diversity in the sediments from urban and industrial areas of the middle reaches is generally higher than that in the upper and lower reaches. RDA analysis indicated that the sediment CEC, TOC, DOC, TP, nitrogen in weak acid extractable form (WAEF-N) and nitrogen in ion exchangeable form (IEF-N) were the key factors driving the differences in the structural characteristics of bacterial communities in the sediment of Nanfei River. The influence of TP was significant on Proteobacteria, Bacteroidetes and Firmicutes, and WAEF-N had a greater influence on Chloroflexi.
杨长明, 吴亚琼, 王育来, 郭韵昊. 南淝河表层沉积物细菌群落结构特征及驱动因素[J]. 中国环境科学, 2018, 38(9): 3552-3561.
YANG Chang-ming, WU Ya-qiong, WANG Yu-lai, GUO Yun-hao. Microbial community structure characteristics and its key driving factors in surface sediments along Nanfei River. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(9): 3552-3561.
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