The influence of the single benzo[a]pyrene pollution or combined pollution with cadmium on the distribution and accumulation of benzo[a]pyrene in subcellular fractions of Eisenia fetida
ZHOU Li-na1,2,3, ZHOU Jing1, LIU Xiao-ya1, LI Hui-xin1, HU Feng1, XU Li1
1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;
2. Jiangsu Organic Solid Waste Resource Cooperative Innovation Center, Nanjing Agricultural University, Nanjing 210095, China;
3. Zhejiang Renxin Testing Research Institute Co., Ltd., Ningbo 315000, China
The influence of the calcium ion channel blocker (LaCl3) or the SH-blocker (Nitrogen-ethylmaleimide, NEM) on the distribution and accumulation patterns of the Benzoapyrene (BaP) in the three subcellular fractions of earthworm Eisenia fetida (Fraction C:associated with the cytosol; Fractions D:associated with granules; and Fraction E:associated with tissue fragments and cell membranes) were explored under the condition of single BaP contamination and the combined contamination of the BaP and cadmium (Cd). The results showed that BaP was mainly accumulated in the Fraction E (about 55.42%~69.96% of the total content) the Fraction D (about 27.91%~32.90% of the total content) and Fraction C (about 2.13%~11.67% of the total content) no matter under single or combined pollution. Under the single pollution of BaP, the addition of LaCl3and NEM could stimulate the BaP accumulation in three above subcellular fractions. While under the combined pollution, the effects were slightly different. LaCl3 could stimulated the BaP accumulation in three above subcellular fractions, but NEM inhibited the BaP accumulation in the Fraction C (from 0.99mg/kg to 0.59mg/kg), and enhanced the BaP accumulation in the Fraction D and E. Therefore, calcium ion channel and the sulfhydryl protein took participate in the BaP distribution in earthworm, and Cd addition changed the BaP distribution compared with the single BaP contamination.
周丽娜, 周静, 刘潇雅, 常大丽, 李辉信, 胡锋, 徐莉. BaP和Cd单一复合对BaP蚯蚓亚细胞分配的影响[J]. 中国环境科学, 2018, 38(4): 1510-1516.
ZHOU Li-na, ZHOU Jing, LIU Xiao-ya, LI Hui-xin, HU Feng, XU Li. The influence of the single benzo[a]pyrene pollution or combined pollution with cadmium on the distribution and accumulation of benzo[a]pyrene in subcellular fractions of Eisenia fetida. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(4): 1510-1516.
Mielke H W, Wang G, Gonzales C R, et al. PAHs and metals in the soils of inner-city and suburban New Orleans, Louisiana, USA[J]. Environmental Toxicology and Pharmacology, 2004, 18(3):243-247.
Hamdi H, Benzarti S, Manusad?ianas L, et al. Bioaugmentation and biostimulation effects on PAH dissipation and soil ecotoxicity under controlled conditions[J]. Soil Biology and Biochemistry, 2007,39(8):1926-1935.
[5]
Wei B G, Yang L S. A review of heavy metal contaminations in urban soils, urban road dusts and agricultural soils from China[J]. Microchemical Journal, 2010,94(2):99-107.
[6]
Organization for Economic Cooperation and Development. No. 207:Earthworm, Acute Toxicity Tests[M]. OECD Guidelines for the Testing of Chemicals, 1984:1-9.
[7]
Shi Y, Shi Y, Wang X, Lu Y, et al. Comparative effects of lindane and deltamethrin on mortality, growth, and cellulase activity in earthworms (Eisenia fetida)[J]. Pesticide Biochemistry and Physiology, 2007,89(1):31-38.
[8]
Lee B T, Shin K H, Kim J Y, et al. Progress in earthworm ecotoxicology[J]. Advanced environmental monitoring. Springer Netherlands, 2008,248-258.
Vijver M G, Vink J P M, Miermans C J H, et al. Oral sealing using glue:a new method to distinguish between intestinal and dermal uptake of metals in earthworms[J]. Soil Biology & Biochemistry, 2003,35(1):125-132.
[12]
Vijver M G, Wolterbeek H Th, Vink J P M, et al. Surface adsorption of metals onto the earthworm Lumbricus rubellus and the isopod Porcellio scaber is negligible compared to absorption in the body[J]. Science of the Total Environment, 2005,340(1):271-280.
[13]
Jager T, F leuren R H, Hogendoorn E A, de Korte G. Elucidating the Routes of Exposure for Organic Chemicals in the Earthworm, Eisenia andrei (Oligochaeta)[J]. Environmental Science & Technology, 2003,37(15):3399-3404.
[14]
Morgan J E, Morgan A J. The distribution and intracellular compart-mentation of metals in the endogeicearthworm Aporrectodea caliginosa sampled from an unpolluted and ametal-contaminated site[J]. Environmental Pollution, 1998,99(2):167-175.
[15]
Stoupalova M, Beklova M, Vavrova M, Stilarkova E, Bednarova I. Influence of polycyclic aromatic hydrocarbons on earthworms Eisenia fetida[J]. Fresenius Environ, 2012,Bull.21.
[16]
Hyötyläïnen T, Oikari A. Bioaccumulation of PAHs from creosote-contaminated sediment in a laboratory-exposed freshwater oligochaete, Lumbriculus variegates[J]. Chemosphere, 2004,57(2):159-164.
[17]
Felten D, Emmerling C. Earthworm burrowing behaviour in 2D terraria with single-and multi-species assemblages[J]. Biology and Fertility of Soils, 2009,45(8):789-797.
[18]
Marques C, Pereira R, Gonçalves F. Using earthworm avoidance behaviour to assess the toxicity of formulated herbicides and their active ingredients on natural soils[J]. Journal of Soils and Sediments, 2009,9(2):137-147.
[19]
Wu S J, Wu E M, Qiu L Q, et al. Effects of phenanthrene on the mortality, growth, and anti-oxidant system of earthworms (Eisenia fetida) under laboratory conditions[J]. Chemosphere, 2011, 83(4):429-434.
[20]
Beaumelle L, Gimbert F, Hedde M, et al. Subcellular partitioning of metals in Aporrectodea caliginosa along agradient of metal exposure in 31field-contaminated soils[J]. Science of the Total Environment, 2015,520:136-145.
[21]
Li L Z, Zhou D M, Wang P, et al. Kinetics of cadmium uptake and subcellular partitioning in the earthworm Eisenia fetida exposed to cadmium-contaminated soil[J]. Archives of Environmental Contamination and Toxicology, 2009a,57(4):718-724.
[22]
Yu S, Lanno R P. Uptake kinetics and subcellular compartmentalization of cadmium in acclimated and unacclimated earthworms (Eisenia andrei)[J]. Environmental Toxicology and Chemistry, 2010,29(7):1568-1574.
[23]
史志明.菲在蚯蚓体内的分布及其对蚯蚓抗氧化防御体系的影响[D]. 南京:南京农业大学, 2013.
[24]
李银生.洛克沙胂对蚯蚓的毒性及生态毒理研究[D]. 广州:华南农业大学, 2003.
[25]
Dingemans M M, Groot A de, Kleef R G van, et al. Hydroxylation increases the neurotoxic potential of BDE-47to affect exocytosis andcalcium homeostasis in PC12 cells[J]. Environmental Health Perspectives, 2008,116(5):637-643.
Li L Z, Zhou D M, Wang P, Luo X S. Subcellular distribution of Cd and Pb in earthworm Eisenia fetida as affected by Ca2+ ions and Cd-Pb interaction[J]. Ecotoxicology and Environmental Safety, 2008,71(3):632-637.
[29]
Vijver M G, Van Gestel C A M, Lanno R P, et al. Internal metal sequestration and its ecotoxicological relevance:a review[J]. Environmental Science & Technology, 2004,38(18):4705-4712.
[30]
Contreras-Ramos S M, Álvarez-Bernal D, Dendooven L. Eisenia fetida increased removal of polycyclic aromatic hydrocarbons from soil[J]. Environmental Pollution, 2006,141(3):396-401.
[31]
Gao Y Z, Cao X Z, Kang F X, et al. PAHs pass through the cell wall and partition into organelles of arbuscular mycorrhizal roots of ryegrass[J]. Journal of Environmental Quality, 2011,40(2):653-656.
[32]
Shi Z M, Xu L, Hu F. A Hierarchic Method for Studying the distribution of phenanthrene in Eisenia fetida[J]. Pedosphere, 2014,24(6):743-752.
[33]
Li L Z, Zhou D M, Peijnenburg W J G M, et al. Uptakepathways and toxicity of Cd and Zn in the earthworm Eisenia fetida[J]. Soil Biology & Biochemistry, 2010,42(7):1045-1050.
[34]
Slaveykova V I, Wilkinson K J. Physicochemical aspects of lead bioaccumulation by Chlorella vulgaris[J]. Environmental Science & Technology, 2002,36(5):969-675.
[35]
Aduayom I, Campbell P G, Denizeau F, Jumarie C. Different transport mechanisms for cadmium and mercury in Caco-2 cells:inhibition of Cd uptake by Hg without evidence for reciprocal effects[J]. Toxicology & Applied Pharmacology, 2003,189(1):56-67.
[36]
Duan X, Xu L, Song J, et al. Effects of benzo[a]pyrene on growth, the antioxidant system, and DNA damage in earthworms (Eisenia fetida) in 2different soil types under laboratory conditions[J]. Environmental Toxicology & Chemistry, 2015,34(2):283-290.
