Study on the removal of Cd(II) by Pseudomonas nitroreducens: Biosorption characteristics and mechanism
YU Yong-quan1,2, HUANG Wei-wei3, DONG Jian-jiang4, ZHU Qi-fa3, LU Dian-nan2, LIU Yong-min1
1. College of Petroleum and Chemical Engineering, Liaoning Shihua University, Fushun 113001, China;
2. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
3. Anhui Wannan Tobacco Liability Company, Xuancheng 242000, China;
4. China Tobacco Corporation, Hefei 230022, China
The adsorption characteristics and adsorption mechanism of cadmium removal by Pseudomonas nitroreducens were investigated systematically. The results showed that the strain could tolerate cadmium up to 200mg/L. Further increase the concentration of cadmium led to a significantly inhibition of the growth of Pseudomonas nitroreducens. In a liquid culture of Pseudomonas nitroreducens, where a liquid LB medium containing 20mg/L, 50mg/L or 100mg/L Cd2+ was used, the removal rate of cadmium reached 94.3% 91.0% and 86.0%, respectively after 120h of cultivation. The impact of pH, temperature, salt concentration and various other heavy metal ions on the removal efficiency of cadmium were also explored. The results showed that Pseudomonas nitroreducens effectively adsorbed cadmium with in the pH range from 4 to 8. The osmosis stress test showed that Pseudomonas nitroreducens could tolerate a NaCl solution of 1mol/L. Under this condition, the strain still adsorbed a variety of heavy metal ions except lead ions. X-ray photoelectron spectroscopy analysis showed that the binding energy of cadmium changed after adsorption. Scanning electron microscopy (SEM) showed that Pseudomonas nitroreducens deformed and has white particles attached on the surface after cadmium treatment, indicating that biomineralization is one of its ways to adsorb heavy metals.
喻涌泉, 黄魏魏, 董建江, 朱启法, 卢滇楠, 刘永民. 硝基还原假单胞菌吸附重金属镉的机理研究[J]. 中国环境科学, 2017, 37(6): 2232-2238.
YU Yong-quan, HUANG Wei-wei, DONG Jian-jiang, ZHU Qi-fa, LU Dian-nan, LIU Yong-min. Study on the removal of Cd(II) by Pseudomonas nitroreducens: Biosorption characteristics and mechanism. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(6): 2232-2238.
Adamis P D B, Panek A D, Leite S G F, et al. Factors involved with cadmium absorption by a wild-type strain of Saccharomyces cerevisiae[J]. Brazilian Journal of Microbiol., 2003,34(1):55-60.
[2]
Perez A L, Anderson K A. D G T estimates cadmium accumulation in wheat and potato from phosphate fertilizer applications[J]. Science of The Total Environment, 2009, 407(18):5096-5103.
Dong J, Mao W H, Zhang G P, et al. Root excretion and plant tolerance to cadmium toxicity-a review[J]. Plant Soil Environ., 2007,53(5):193-200.
[5]
Alkorta I, Hernández A J. Becerril J M et al. Recent findings on the phytoremediation of soils contaminated with environmentally toxic heavy metals and metalloids such as zinc, cadmium, lead, and arsenic[J]. Reviews in Environmental Science and Bio/Technology, 2004,3:71-90.
[6]
Hossain Z, Huq F. Studies on the interaction between Cd2+ ions and nucleobases and nucleotides[J]. Journal of Inorganic Biochemistry, 2002,90(3/4):97-105.
Ahluwalia S S, Goyal D. Microbial and plant derived biomass for removal of heavy metals from wastewater[J]. Bioresource Technology, 2007,98(12):2243-2257.
Riggle P J, Kumamoto C A. Role of a Candida albicans P1-type ATPase in resistance to copper and silver ion toxicity[J]. Journal of Bacteriology, 2000,182(17):4899-4905.
Figueira E, Lima A I G, Pereira S I A. Cadmium tolerance plasticity in Rhizobium leguminosarum bv.viciae:glutathione as a detoxifying agent[J]. Canadian Journal of Microbiology, 2005, 51(1):7-14.
[22]
Gharieb M M, Wilkinson S C, Gadd G M. Reduction of selenium oxyanions by unicellular, polymorphic and filamentous fungi:cellular location of reduced selenium and implications for tolerance[J]. Journal of Industrial Microbiology, 1995,14(3):300-311.
[23]
Neumann G, Veeranagouda Y, Karegoudar T B, et al. Cells of Pseudomonas putida andEnterobacter sp. adapt to toxic organic compounds by increasing the size[J]. Extremophiles, 2005,9(2):163-168.
[24]
Nithya C, Gnanalakshmi B, Pandian S K. Assessment and characterizationof heavy metal resistance in Palk Bay sediment bacteria[J]. Marine Environmental Research, 2011,71(4):283-294.
[25]
Brady D, Duncan J R. Bioaccumulation of metal cations by Saccharomyces cerevisiae[J]. Applied Microbiology and Biotechnology, 1994.41(1):149-159.
[26]
Karpel R T, Alon, G, Glaser, S S et al. Expression of sodium proton antiporter (NhaA) in Escherichia coli is induced by Na1and Li1ions[J]. Journal of Biological Chemistry, 1991, 266(32):21753-21759.
[27]
Kiran I, Akar T, Ozca A S, et al. Biosorption kinetics and isotherm studies of Acid Red 57by dried Cephalosporium aphidicola cells from aqueous solutions[J]. Biochemical Engineering Journal, 2006,31(3):197-203.
[28]
Kapoor A, Viraraghavaa T, Cullimore R D. Removal of heavy metals using the fungus Aspergillus niger[J]. Bioresource Technology, 1999,70(1):95-104.
Fan T, Liu Y G, Feng B Y, et al. Biosorption of cadmium (Ⅱ), zinc (Ⅱ) and lead (Ⅱ) by Penicillium simplicissimum:Isotherms, kinetics and thermodynamics[J]. Journal of Hazardous Materials, 2008,160(2/3):655-661.
[31]
Fu C, Maier R J. Competitive inhibition of an energy-dependent nickel transport system by divalent cations in Bradyrhizobium japonicum JH[J]. Appliedand Enviroment Micrology, 1991,57:3511-3516.
[32]
Pabst M W, Miller C D, Dimkpa C O, Anderson A J, et al. Defining the surface adsorption and internalization of copper and cadmium in a soil bacterium, Pseudomonas putida[J]. Chemosphere, 2010,81(7):904-910.
[33]
Schalk I J, Hannauer M and Braud A. New roles for bacterial siderophores in metal transport and tolerance[J]. Environmental Microbiology, 2011,13(11):2844-2854.
