Inhibitory kinetics of free nitrous acid on Nitrobacter
SUN Hong-wei1,2, YU Xue1,2, LI Wei-wei3, QI Guo-ping3, MA Juan1,2, CHENG Yong-zhi1,2, LV Xin-tao4
1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
2. Gansu Sewage Treatment Industry Technical Center, Lanzhou 730070, China;
3. Gansu Province Light Industry Research Institute, Lanzhou 730070, China;
4. Research and Development Center of Beijing Drainage Group Technology, Beijing 100022, China
A sequencing batch reactor (SBR) was operated in this study to investigate the inhibitory kinetics of free nitrous acid (FNA) on Nitrobacter. At the beginning of the experiment, FNA concentration in influent was changed to enrich Nitrobacter. Then, the sludge of enrichment Nitrobacter was employed to study the variation law of the specific nitrite oxidation rate (SNiOR) during nitrite oxidation process of batch tests. Meanwhile, metagenomic species annotation and abundance analysis showed that Nitrobacter accounted for 40.3% of the total bacterial population. Furthermore, kinetic model of FNA inhibition on Nitrobacter activity was fitted for statistical analysis. The results showed that the SNiOR increased rapidly with the increase of FNA concentration when ≤0.1·mg/L while decreased with the increase of FNA concentration as FNA>0.1mg/L. In particular, the SNiOR was maintained at 0gN/(gVSS·d) when FNA concentration was higher than 0.7mg/L, implying that Nitrobacter activity was completely inhibited. Statistical analysis results showed that compared to Haldane, Aiba, Edwards-1#, Edwards-2# and Luong inhibition kinetics models, Han-Levenspiel model was the most suitable one for describing the inhibitory effect of FNA on Nitrobacter activity. The statistical constants, e.g., residual square sum (RSS) correlation coefficient (R2), F value of the analysis of variance and confidence degree (P) was 0.02, 0.90, 78.1and 3.29×10-12, respectively. The dynamic constant values, e.g., maximum specific nitrite oxidation rate (rmax), half saturation constant (KS) and critical inhibition constant (Sm) was 1.57gN/(gVSS·d), 0.01mg/L and 0.66mg/L, respectively.
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