Research progress of microbial source tracking based on Bacteroidales 16S rRNA gene
LIANG Hong-xia1, YU Zhi-sheng1, LIU Ru-yin1, ZHANG Hong-xun1, WU Gang2
1. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China;
2. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
Microbial source tracking (MST) is a method for distinguishing fecal contamination from different animals by identifying specific fecal microbes. Bacteroidales is widely used in MST because of its high abundance in feces, non-reproduction in vitro, and host specificity. Given that Bacteroidales 16S rRNA gene is a common biomarker for MST, this paper reviewed the decay of Bacteroidales and its biomarkers, the sensitivity and specificity of the 16S rRNA gene primers for Bacteroidales, and the application of molecular techniques in MST. It will provide the appropriate scientific reference for the source apportionment of feces.
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