The effects of system temperature and DO level in aerobic reactor on nitrification and denitrification in three lab-scale moving bed biofilm reactors(MBBRs)in series operated under anoxic/aerobic/aerobic conditions respectively, were investigated. The microscopic mechanism based on the changes in the structure of nitrogen removal functional bacteria in biofilm affected by temperature and DO were discussed using high-throughput sequencing technology. The results indicated that the increase in system temperature could enhance not only nitrification but also denitrification process in biofilm, and the increase in the DO level in aerobic reactor was beneficial to nitrification process. During the continuous operation of the processes, when the system temperature and the DO level in the aerobic reactor were at the highest level (i.e., temperature=20~22℃, DO=5~8mg O2/L), more than 1.60g NH4+-N/(m2·d) of the specific nitrification loading rate could be achieved, and the specific denitrification loading rate was as high as 2.84g NO3--N/(m2·d). The optimal removal efficiencies of NH4+-N and TN could reach 98.7% and 85.7% respectively. The essential reasons for the changes in the population and community structure of nitrogen removal functional bacteria resulted from the variation in temperature and DO level. When the DO level in the aerobic reactor decreased, the OTUs proportion of nitrifiers, especially heterotrophic nitrifiers, decreased significantly. The effect from the temperature on the denitrifiers could be mainly attributed to the structural change in the community.
魏小涵, 毕学军, 尹志轩, 周小琳, 徐晨璐, 葛文杰. 温度和DO对MBBR系统硝化和反硝化的影响[J]. 中国环境科学, 2019, 39(2): 612-618.
WEI Xiao-han, BI Xue-jun, YIN Zhi-xuan, ZHOU Xiao-lin, XU Chen-lu, GE Wen-jie. Effects of temperature and dissolved oxygen on nitrification and denitrification in MBBR system. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(2): 612-618.
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